Protein encapsulation and release from degradable sugar based hydrogels

Heyden, Karen Van der; Babooram, Keshwaree; Ahmed, Marya; Narain, Ravin

doi:10.1016/j.eurpolymj.2009.03.005

Cited by 21 publications

(14 citation statements)

References 32 publications

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“…Hydrogels based on carbohydrate monomers are capable of sorbing a large amount of water, thereby making them suitable for biomedical applications. Poly(N‐isopropylacrylamide‐co‐galactosylated acrylate) hydrogels have been used as cell culture media with thermally‐induced cell detachment, while hydrogels based on D‐gluconamidoethyl methacrylate and N‐isopropylacrylamide have been used for protein encapsulation and release . Hemoglobin‐imprinted hydrogels based on vinyl glucosamine are capable of adsorbing significant amounts of hemoglobin and offer the potential to be used as specific recognition units in biosensors .…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

Perin

Felisberti

2018

J of Chemical Tech & Biotech

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BACKGROUND Carbohydrates are important renewable raw materials and their modification by enzymatic reactions with polymerizable groups is of great importance due the possibility of production of polymers with properties for biomedical applications. In this study, D‐fructose‐ and D‐glucose‐based monomers were prepared by enzymatic reactions and structurally characterized. Moreover, hydrogels based on poly(methacryloyl‐D‐fructose) were also prepared and characterized. RESULTS The conversions of 99% and 34% for D‐fructose and D‐glucose, respectively, were achieved using 2,2,2‐trifluoroethyl methacrylate as the methacrylic donor and commercial lipase Novozyme® 435 (EC 3.1.1.3) as enzymatic catalyst in t‐butanol. The molar ratios of D‐fructose and D‐glucose mono‐ and dimethacrylate were 57/43 and 87/13, respectively. These monomers are an isomeric mixture related to the tautomeric equilibrium of the carbohydrates in solution. Methacryloyl‐D‐fructose was polymerized in the presence of a controlled amount of ethyleneglycol dimethacrylate as crosslinker achieving hydrogels with different crosslinking densities which are mechanically stable when subject to compression–decompression cycles and have a high water swelling capability. CONCLUSION The combination of 2,2,2‐trifluoroethylmethacrylate and Novozym® 435 in a heterogeneous media lead to the continuous carbohydrate solubilization and reaction with the formation of carbohydrates‐based monomers. The control of the monomer functionality enables the synthesis of hydrogels with different crosslinking densities that tune their properties. © 2017 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

Perin

Felisberti

2018

J of Chemical Tech & Biotech

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“…29,33,[39][40][41] Amongst these, sugar-based hydrogels are of particular interest as they show minimal toxicity compared to conventional hydrogels and can potentially serve as a targeting ligand in the human body. 4,42 In this study, we aim to explore the possible application of pachyman as a safe and effective drug carrier for the controlled release of protein drugs. The structure of the hydrogel was characterized by infrared spectroscopy and the morphology was observed under a scanning electron microscope (SEM).…”

Section: Introductionmentioning

confidence: 99%

A novel pH-responsive hydrogel based on natural polysaccharides for controlled release of protein drugs

Zhong

et al. 2015

RSC Adv.

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A biocompatible and biodegradable hydrogel based on a natural polysaccharide was prepared and characterized to evaluate its applicability as an effective carrier for controlled protein delivery. The hydrogel exhibited significant pH-sensitivity, which was favorable for protein release in a simulated intestinal medium. It is capable of incorporating considerable amounts of protein drugs (encapsulation efficiency up to 97.6 wt%) following a protein-friendly preparation procedure. It was shown that the hydrogel is able to release two entrapped model protein drugs (bovine serum albumin and lysozyme) in a controlled manner with full preservation of protein stability and enzymatic activity for lysozyme.Moreover, the insulin-loaded hydrogel was effective in reducing blood glucose level in diabetic animal models. Importantly, the hydrogel showed no evidence of cytotoxicity in vitro and in vivo, rather, it is biodegradable. The synthesized hydrogel shows favorable features as a promising delivery carrier candidate for targeted delivery of protein drugs to specific sites.

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“…28,29 Applications reported in the literature for hydrogels based on carbohydrate are mainly related to drug delivery systems. 12,[30][31][32][33][34][35][36] However, they have also been used as stationary phase in high-performance liquid chromatography 37 and as cell culture media. 38 Crosslinking agents play a key role on the mechanical and physical chemical properties of the hydrogels.…”

Section: Introductionmentioning

confidence: 99%

“…39,40 Crosslinker may also influence hydrogel degradation, being able to render biodegradability to conventionally non-biodegradable polymers or impart the hydrogel pH-sensitivity, for instance. 32,41,42 However, the replacement of conventional crosslinker based on fossil resources to those based on carbohydrates avoids or minimizes cytotoxicity. 15,43 Nevertheless, synthesis of hydrogels fully based on carbohydrate monomers and crosslinkers are still barely reported.…”

Section: Introductionmentioning

confidence: 99%

Macroporous hydrogels based on carbohydrates monomethacrylates and dimethacrylates: singular properties from carbohydrate‐based crosslinkers

Perin

Fonseca

Felisberti

2019

J of Chemical Tech & Biotech

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BACKGROUND Readily available feedstock and biocompatibility make carbohydrate‐based hydrogels promising materials for biomedical applications. However, carbohydrate‐based crosslinkers are rather underexplored when compared with crosslinkers derived from fossil resources. In this study, novel fully bio‐based hydrogels derived from enzymatically produced d‐fructose and d‐glucose methacrylate monomers were synthesized with different amounts of d‐fructose dimethacrylate crosslinker. RESULTS The use of a carbohydrate‐based crosslinker endows hydrogels with high swelling coefficients, up to 2400%, and superior mechanical resistance (compressive modulus up to 9.5 kPa with a maximum stress up to 50 kPa) compared with conventional crosslinkers based on fossil resources. Hydrogels shape and crosslinking density influences hydrogel morphology, swelling behavior and mechanical resistance. Moreover, hydrogels presented cell viability, biodegradability and hydrolysis‐resistance over a wide range of pH. CONCLUSION The use of a highly hydrophilic crosslinker based on carbohydrate for hydrogels synthesis enables the use of high crosslinker concentration, which improves mechanical properties, however with minor loss of the water swelling capacity, compared with conventional fossil‐based crosslinkers. This is an important advantage over conventional crosslinkers based on fossil resources. Moreover, slab hydrogels hold higher stress under compression–decompression cycles, and present higher resistance to hydrolysis in basic medium due to the thicker pore walls than cylindrical ones. © 2019 Society of Chemical Industry

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Protein encapsulation and release from degradable sugar based hydrogels

Cited by 21 publications

References 32 publications

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

A novel pH-responsive hydrogel based on natural polysaccharides for controlled release of protein drugs

Macroporous hydrogels based on carbohydrates monomethacrylates and dimethacrylates: singular properties from carbohydrate‐based crosslinkers

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