Protein Electrophoresis in Hermann's Tortoises (Testudo hermanni): A First Step in Reference Value Determination

Fiorucci, Letizia; Ceccarelli, Marcello; Macrelli, Roberto; Crosta, Lorenzo

doi:10.5818/1529-9651-23.1.15

Cited by 8 publications

(2 citation statements)

References 13 publications

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“…For mid-to long-term storage, freezing is preferred over room temperature or þ48C (þ39.28F) storage to maintain protein stability. 9,12,13 Within the Sauropsida clade, multiple published studies about plasma or serum electrophoresis have used samples stored in a conventional freezer (À208C [À48F]) [14][15][16][17][18][19][20] or in an ultrafreezer (À70 to À808C [À94 to À1128F]) 6,[21][22][23][24][25][26] for a few days up to a dozen years. For a given study, storage time can differ between samples, sometimes by years.…”

Section: Introductionmentioning

confidence: 99%

Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (Ciconia ciconia)

Thorel,

Roman,

Leclerc

2023

Journal of Avian Medicine and Surgery

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Plasma electrophoresis is an ancillary diagnostic tool in avian medicine, with agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) being the most common techniques. Frozen samples can be used for quantitative studies or comparative diagnostic purposes, but stability of avian plasma proteins under freezing is poorly described. To evaluate the influence of plasma freezing on electrophoretograms in white storks (Ciconia ciconia), heparin blood was sampled from 30 individuals during annual health examinations. Plasma samples were obtained after centrifugation of fresh samples and divided into aliquots. Both AGE and CZE were performed on fresh aliquots. The remaining aliquots were frozen at –20°C (–4°F) or –180°C (–292°F) and thawed following different protocols: 1 freeze/thaw cycle after 6 months at –20°C; 1, 2, 4, and 7 cycles over 12 months at –20°C; and 1 cycle after 18 months at –180°C. For both techniques, electrophoretic profiles obtained from these thawed aliquots were compared to fresh electrophoretograms. Quantitatively, significant differences (P < 0.05) in most fractions were seen from 6 months postfreezing at –20°C for both techniques. Fewer statistically significant differences were observed after 18 months under cryogenic preservation (–180°C). Qualitatively, AGE provided more repeatable and stable results than CZE over time on samples stored at –20°C, and electrophoretograms were stable after 18 months of cryogenic storage. An electromigration distortion associated with freezing was seen with CZE only. Plasma samples stored in a conventional freezer (–20°C) should not be compared to fresh plasma. For quantitative studies, cryogenic storage should be privileged.

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Section: Introductionmentioning

confidence: 99%

Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (Ciconia ciconia)

Thorel,

Roman,

Leclerc

2023

Journal of Avian Medicine and Surgery

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“…albumin or prealbumin, alpha, beta and gamma globulins) have been observed in reptiles (Girling 2010). Reference values have been published for a limited number of reptile species (Deem et al 2006;Deem et al 2009;Gimenez et al 2010;Cooper-Bailey et al 2011;Proverbio et al 2012;Fiorucci et al 2013;Andreani et al 2014;Flower et al 2014, Musilova et al 2015 changes in the proportions of plasma protein fractions in reptile patients were described only in some studies (Gimenez et al 2010;Moore et al 2014). The aim Supported by the Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno (Institucionální výzkum FVL VFU Brno, VFU FVL/1650/29).…”

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confidence: 99%

Plasma protein electrophoresis in green iguanas (Iguana iguana) suffering from hepatic, renal and inflammatory skin diseases

Knotková¹,

Musilová²,

Pinterova³

et al. 2019

Vet. Med. - Czech

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The goals of this study were to evaluate electrophoretograms in green iguanas suffering from hepatic, renal and inflammatory skin diseases. Plasma samples were obtained from 14 green iguana patients. The diagnosis was based on clinical examination, evaluation of biochemical and haematological profiles or histopathological examination. Agarose gel electrophoresis was performed using an automated HYDRASYS system. A decrease in the albumin/globulin ratio was observed in 11 out of 14 patients (three out of four patients with liver failure, one out of one patient with chronic renal insufficiency and suspected hepatopathy, two out of two patients with chronic interstitial nephritis, four out of five patients with chronic renal insufficiency and one out of two patients with dermatitis). The alpha globulin fraction was elevated in 1 out of 14 patients (patient with dermatitis). The fraction of beta globulins was increased in 8 out of 14 patients (two out of four patients with liver diseases, four out of eight patients with various forms of renal failure and two out of two patients with inflammatory skin diseases). Increased gamma globulin levels were observed in 4 out of 14 patients (one out of four patients with impaired function of the liver, two out of eight patients with renal diseases and one out of two patients with dermatitis). Changes in the electrophoretograms of green iguanas are not disease-specific, but in conjunction with other biochemical blood tests the electrophoresis of plasma proteins may be a useful indicator in the diagnosis of metabolic and infectious diseases and in the monitoring of disease progress.

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Evidence-Based Advances in Reptile Medicine

Mitchell

Perry

2017

Veterinary Clinics of North America: Exotic Animal Practice

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Protein Electrophoresis in Hermann's Tortoises (Testudo hermanni): A First Step in Reference Value Determination

Cited by 8 publications

References 13 publications

Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (Ciconia ciconia)

Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (Ciconia ciconia)

Plasma protein electrophoresis in green iguanas (Iguana iguana) suffering from hepatic, renal and inflammatory skin diseases

Evidence-Based Advances in Reptile Medicine

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