Protein disulfide isomerases: Impact of thapsigargin treatment on their expression in melanoma cell lines

Silva, Zélia; Veríssimo, Teresa; Videira, Paula A.; Novo, Carlos

doi:10.1016/j.ijbiomac.2015.04.029

Cited by 6 publications

(7 citation statements)

References 25 publications

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“…In the present study, the cell models used reflected the potential applications of DES in the cosmetics and skin care industry. HaCaT 32–35 and MNT-1 36–38 cell lines are widely used as human skin cell models. Surprisingly, given the previous records found in literature (see above), the tested DES did not show cytotoxicity with the exception of all [N 4444 ]Cl-based DES, and many were able to stimulate cell viability instead, especially in non-tumoral cells.…”

Section: Discussionmentioning

confidence: 99%

“…This work aims to assess the cytotoxicity of a set of DES towards two human skin cell lines, HaCaT (keratinocytes cells) and MNT-1 (melanoma cells), considering the increased relevance of these solvents in many sectors of industry. HaCaT 32–35 , in particular, was chosen as model for cosmetic applications, while MNT-1 36–38 was selected as a model to understand the potential of the DES under study on the treatment of skin disorders. The DES effects on the cell viability were assessed through the MTT assay.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxicity profiling of deep eutectic solvents to human skin cells

Macário

Oliveira

Menezes

et al. 2019

Sci Rep

103

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The tailor-made character of deep eutectic solvents (DES) turns them very attractive to be used in several applications, including in health-related areas such as pharmaceutical, nutraceutical, and cosmetic industries. However, although DES has been touted as “green” solvents, several works proved that their potential toxicity should not be neglected. Using the premise of DES applicability in the cosmetic and pharmaceutical sectors, we chose two cell lines to work as a skin model (keratinocytes HaCaT and tumor melanocytes MNT-1), to assess DES cytotoxicity. The effect of three different hydrogen bond acceptors (HBA) ([Chol]Cl, [N 1111 ]Cl and [N 4444 ]Cl) and three different hydrogen bond donors (HBD) (hexanoic and butanoic acid, ethylene glycol, 1-propanol and urea) were evaluated through a common viability assay (MTT assay). Results were promising since [Chol]Cl and [N 1111 ]Cl- based DES showed good biocompatibility for the tested cells. [N 4444 ]Cl-based DES, however, showed cytotoxicity for both cell lines, with the HBA being the driver of the toxicity. Interestingly, some compounds increased cell viability in the HaCaT cell line, namely [Chol]Cl, ethylene glycol, hexanoic acid, urea, and all [Chol]Cl and [N 1111 ]Cl-based DES and should be considered as targets for future studies. These results highlight their possible use in cosmetic or pharmaceutical formulations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxicity profiling of deep eutectic solvents to human skin cells

Macário

Oliveira

Menezes

et al. 2019

Sci Rep

103

View full text Add to dashboard Cite

show abstract

“…Consistent with this, there is dysregulated expression of KLH29, RAB35 and TCPR1 proteins, which are involved in fusing endosomes to lysosomes. Moreover, the expression of ER stress related proteins TMX4 [64], REEP1 [65], TNFAIP8 [66]) and cell death associated proteins (TNFRSF10B [67], TRAIL [68], suggest that the PM has disrupted ER-phagosome interactions [69] and induced an ER stress-like response.…”

Section: Discussionmentioning

confidence: 99%

Exposure of trophoblast cells to fine particulate matter air pollution leads to growth inhibition, inflammation and ER stress

et al. 2019

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Ambient air pollution is considered a major environmental health threat to pregnant women. Our previous work has shown an association between exposure to airborne particulate matter (PM) and an increased risk of developing pre-eclamspia. It is now recognized that many pregnancy complications are due to underlying placental dysfunction, and this tissue plays a pivotal role in pre-eclamspia. Recent studies have shown that PM can enter the circulation and reach the human placenta but the effects of PM on human placental function are still largely unknown. In this work we investigated the effects of airborne PM on trophoblast cells. Human, first trimester trophoblast cells (HTR-8/SV) were exposed to urban pollution particles (Malmö PM2.5; Prague PM10) for up to seven days in vitro and were analysed for uptake, levels of hCGβ and IL-6 secretion and proteomic analysis. HTR-8/SVneo cells rapidly endocytose PM within 30 min of exposure and particles accumulate in the cell in perinuclear vesicles. High doses of Prague and Malmö PM (500–5000 ng/ml) significantly decreased hCGβ secretion and increased IL-6 secretion after 48 h exposure. Exposure to PM (50 ng/ml) for 48h or seven days led to reduced cellular growth and altered protein expression. The differentially expressed proteins are involved in networks that regulate cellular processes such as inflammation, endoplasmic reticulum stress, cellular survival and molecular transport pathways. Our studies suggest that trophoblast cells exposed to low levels of urban PM respond with reduced growth, oxidative stress, inflammation and endoplasmic reticulum stress after taking up the particles by endocytosis. Many of the dysfunctional cellular processes ascribed to the differentially expressed proteins in this study, are similar to those described in PE, suggesting that low levels of urban PM may disrupt cellular processes in trophoblast cells. Many of the differentially expressed proteins identified in this study are involved in inflammation and may be potential biomarkers for PE.

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“…It also evokes ROS generation in cells through calcium-ion mediated changes (mitochondrial depolarization), which can result in cell dysfunction and damage [242,243]. In addition, compound 13 is known for its cytotoxic action against different cancer cell-lines, for instance melanoma [244], insulinoma [245,246], neuronal [247], and breast [248].…”

Section: Thapsigarginmentioning

confidence: 99%

Applications of Sesquiterpene Lactones: A Review of Some Potential Success Cases

et al. 2020

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Sesquiterpene lactones, a vast range of terpenoids isolated from Asteraceae species, exhibit a broad spectrum of biological effects and several of them are already commercially available, such as artemisinin. Here the most recent and impactful results of in vivo, preclinical and clinical studies involving a selection of ten sesquiterpene lactones (alantolactone, arglabin, costunolide, cynaropicrin, helenalin, inuviscolide, lactucin, parthenolide, thapsigargin and tomentosin) are presented and discussed, along with some of their derivatives. In the authors’ opinion, these compounds have been neglected compared to others, although they could be of great use in developing important new pharmaceutical products. The selected sesquiterpenes show promising anticancer and anti-inflammatory effects, acting on various targets. Moreover, they exhibit antifungal, anxiolytic, analgesic, and antitrypanosomal activities. Several studies discussed here clearly show the potential that some of them have in combination therapy, as sensitizing agents to facilitate and enhance the action of drugs in clinical use. The derivatives show greater pharmacological value since they have better pharmacokinetics, stability, potency, and/or selectivity. All these natural terpenoids and their derivatives exhibit properties that invite further research by the scientific community.

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Protein disulfide isomerases: Impact of thapsigargin treatment on their expression in melanoma cell lines

Cited by 6 publications

References 25 publications

Cytotoxicity profiling of deep eutectic solvents to human skin cells

Cytotoxicity profiling of deep eutectic solvents to human skin cells

Exposure of trophoblast cells to fine particulate matter air pollution leads to growth inhibition, inflammation and ER stress

Applications of Sesquiterpene Lactones: A Review of Some Potential Success Cases

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