Protein diffusion in a bicontinuous microemulsion: inducing sub-diffusion by tuning the water domain size

Neubauer, Ralph; Höhn, Sebastian; Dulle, Martin; Lapp, Alain; Schulreich, Christoph; Hellweg, Thomas

doi:10.1039/c6sm02107g

Cited by 12 publications

(8 citation statements)

References 46 publications

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“…FCS is very well suited for microemulsion systems, as has been shown for W/O MEs, 108,109 bicontinuous MEs, 110 and O/W MEs, 111,112 allowing one to deduce size and size distribution of microemulsion droplets. 29 Since here only the motion of fluorescently labeled particles is measured, FCS also allows to measure the mobility of different fluorescently labeled compounds or additives of a microemulsion.…”

Section: Survey Of Techniques For Characterizing Microemulsionsmentioning

confidence: 99%

“…For bicontinuous microemulsions this method then enables one to determine the size of the water domains when using a probe like green fluorescent protein (GFP). 110 In addition, to FCS one can also employ the time-resolved decay of fluorescence to determine the aggregation number of droplet-type microemulsions. 113 Furthermore, fluorescence probes can be employed to characterize the local polarity within the domains of a microemulsion, 114 an aspect that can be particularly interesting when investigating microemulsions based on ionic liquids.…”

Section: Survey Of Techniques For Characterizing Microemulsionsmentioning

confidence: 99%

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Using Microemulsions: Formulation Based on Knowledge of Their Mesostructure

et al. 2021

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Microemulsions, as thermodynamically stable mixtures of oil, water, and surfactant, are known and have been studied for more than 70 years. However, even today there are still quite a number of unclear aspects, and more recent research work has modified and extended our picture. This review gives a short overview of how the understanding of microemulsions has developed, the current view on their properties and structural features, and in particular, how they are related to applications. We also discuss more recent developments regarding nonclassical microemulsions such as surfactant-free (ultraflexible) microemulsions or ones containing uncommon solvents or amphiphiles (like antagonistic salts). These new findings challenge to some extent our previous understanding of microemulsions, which therefore has to be extended to look at the different types of microemulsions in a unified way. In particular, the flexibility of the amphiphilic film is the key property to classify different microemulsion types and their properties in this review. Such a classification of microemulsions requires a thorough determination of their structural properties, and therefore, the experimental methods to determine microemulsion structure and dynamics are reviewed briefly, with a particular emphasis on recent developments in the field of direct imaging by means of electron microscopy. Based on this classification of microemulsions, we then discuss their applications, where the application demands have to be met by the properties of the microemulsion, which in turn are controlled by the flexibility of their amphiphilic interface. Another frequently important aspect for applications is the control of the rheological properties. Normally, microemulsions are low viscous and therefore enhancing viscosity has to be achieved by either having high concentrations (often not wished for) or additives, which do not significantly interfere with the microemulsion. Accordingly, this review gives a comprehensive account of the properties of microemulsions, including most recent developments and bringing them together from a united viewpoint, with an emphasis on how this affects the way of formulating microemulsions for a given application with desired properties.

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Section: Survey Of Techniques For Characterizing Microemulsionsmentioning

confidence: 99%

Section: Survey Of Techniques For Characterizing Microemulsionsmentioning

confidence: 99%

Using Microemulsions: Formulation Based on Knowledge of Their Mesostructure

et al. 2021

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“…Although VE-BODIPY has a slightly higher molecular weight (747.86) than that of natural VE (430.79), these values are in good agreement with the results obtained with electrochemical measurements (Figure b). In contrast, Neubauer and co-workers were the first to investigate the diffusion of an enhanced green fluorescent protein (GFP+) in a BME (water/sugar surfactant + 1-pentanol/cyclohexane) estimated by FCS measurement . They reported that the GFP+ diffusion slowed as the water domain size in the BME decreased.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, Neubauer and coworkers were the first to investigate the diffusion of an enhanced green fluorescent protein (GFP+) in a BME (water/ sugar surfactant + 1-pentanol/cyclohexane) estimated by FCS measurement. 41 They reported that the GFP+ diffusion slowed as the water domain size in the BME decreased. Very interestingly, our study (lipophilic VE diffusion through the micro-oil phase in a BME) was conceptually opposite to their study (hydrophilic GFP+ diffusion through the micro-water phase in a BME), but the tendency of the diffusion of each species in the BME was the same.…”

Section: T H Imentioning

confidence: 99%

Lipophilic Vitamin E Diffusion through Bicontinuous Microemulsions

et al. 2021

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We studied the diffusion properties of lipophilic vitamin E (VE) through bicontinuous microemulsions (BME) using both electrochemical and fluorescence correlation spectroscopy (FCS) measurements. We investigated the effect of different composition ratios of micro-water and micro-oil phases in BMEs (W/O BME ). When we employed the BME with a lower W/O BME value of 40/60 (oil-rich BME) as an electrolyte solution, we obtained a larger current response from VE at a fluorinated nanocarbon film electrode. Further voltammetric studies revealed that a higher VE diffusion coefficient was observed in the oil-rich BME. The FCS results also exhibited faster diffusion through the oil-rich BME, which played a significant role in accelerating the VE diffusion probably due to the widening of the micro-oil phase pathway in the BME. Moreover, the effect of increasing the VE diffusion was pronounced at the interface between the electrode surface and the BME solution. These results indicate that controlling the conditions of the BME as the measurement electrolyte is very effective for achieving superior electrochemical measurements in a BME.

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“…Neubauer and co-workers were the first to use FCS measurement to investigate the diffusion of an enhanced green fluorescent protein (GFP+) in a BME (water/sugar surfactant+1-pentanol/cyclohexane). 25 They reported that the GFP+ diffusion slowed as the water domain size in the BME decreased.…”

Section: Fcs Measurementsmentioning

confidence: 99%

Lipophilic probe behavior in microemulsions evaluated by fluorescence correlation spectroscopy

et al. 2022

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We evaluated the dispersion and diffusion of fluorescent-labeled lipophilic vitamin E (VE) in microemulsions (MEs) including water-in-oil (W/O) type ME, oil-in-water (O/W) type ME and bicontinuous ME (BME), by using fluorescence correlation spectroscopy (FCS). We prepared a fluorescent ATTO 488 or BODIPY group labeled VE (VE-ATTO or VE-BODIPY). VE-ATTO possesses lipophilic and hydrophilic parts, while VE-BODIPY consists solely of the lipophilic part. The VE-ATTO dissolved in heptane solution as an oil phase, appeared hot pink in color due to the solvatochromism effect under room light and almost no fluorescent signal, which was unlike the VE-ATTO dissolved in ME solutions and all the VE-BODIPY solutions (typical fluorescent green color). The FCS measurement rapidly proved that VE-BODIPY diffuses faster than VE-ATTO. This is presumably because the "surfactant-like" VE-ATTO is localized and trapped at the micro-water/micro-oil interface of the MEs, while the VE-BODIPY exists in the ME phase and macro-oil phase with good dispersion. These results demonstrate that FCS is a powerful tool for the rapid evaluation of the lipophilic probe behavior in heterogeneous ME solutions.

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Protein diffusion in a bicontinuous microemulsion: inducing sub-diffusion by tuning the water domain size

Cited by 12 publications

References 46 publications

Using Microemulsions: Formulation Based on Knowledge of Their Mesostructure

Using Microemulsions: Formulation Based on Knowledge of Their Mesostructure

Lipophilic Vitamin E Diffusion through Bicontinuous Microemulsions

Lipophilic probe behavior in microemulsions evaluated by fluorescence correlation spectroscopy

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