Protein detection enabled using functionalised silk-binding peptides on a silk-coated optical fibre

Capon, Patrick K.; Horsfall, Aimee J.; Li, Jiawen; Schartner, Erik P.; Khalid, Asma; Purdey, Malcolm S.; McLaughlin, Robert A.; Abell, Andrew D.

doi:10.1039/d1ra03584c

Cited by 2 publications

(1 citation statement)

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“…This hindrance of the diffusion of large size analyte species through a coating with embedded fluorophore hampers the sensing of large molecules such as proteins. To overcome this challenge, a new silk-based 2-step fiber coating method was developed, and its sensing performance was demonstrated using the biotin-streptavidin sensing model [ 27 ]. First, the fiber tip was coated with silk fibroin only, then the outer surface of the coating was decorated with SBP covalently bonded to biotin.…”

Section: Recent Developments Of Single-fiber-based Probes For Combine...mentioning

confidence: 99%

Single-fiber probes for combined sensing and imaging in biological tissue: recent developments and prospects

Li,

Warren-Smith,

McLaughlin

et al. 2024

Biomed. Opt. Express

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Single-fiber-based sensing and imaging probes enable the co-located and simultaneous observation and measurement (i.e., ‘sense’ and ‘see’) of intricate biological processes within deep anatomical structures. This innovation opens new opportunities for investigating complex physiological phenomena and potentially allows more accurate diagnosis and monitoring of disease. This prospective review starts with presenting recent studies of single-fiber-based probes for concurrent and co-located fluorescence-based sensing and imaging. Notwithstanding the successful initial demonstration of integrated sensing and imaging within single-fiber-based miniaturized devices, the realization of these devices with enhanced sensing sensitivity and imaging resolution poses notable challenges. These challenges, in turn, present opportunities for future research, including the design and fabrication of complex lens systems and fiber architectures, the integration of novel materials and other sensing and imaging techniques.

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Section: Recent Developments Of Single-fiber-based Probes For Combine...mentioning

confidence: 99%

Single-fiber probes for combined sensing and imaging in biological tissue: recent developments and prospects

Li,

Warren-Smith,

McLaughlin

et al. 2024

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

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A Sandwich Structure Sensor Based on Ag-Silk Fibroin-Ag Co-Modification for Trace Water Determination in Visible Light Band

Liu

Zhao

et al. 2022

IEEE Sensors J.

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Protein detection enabled using functionalised silk-binding peptides on a silk-coated optical fibre

Abstract: We report a new approach to functionalise optical fibres to enable protein sensing, which controls the sensor molecule location either within the fibre tip coating or isolated to its exterior. This control dictates suitability for protein sensing.

Cited by 2 publications

References 42 publications

Single-fiber probes for combined sensing and imaging in biological tissue: recent developments and prospects

Single-fiber probes for combined sensing and imaging in biological tissue: recent developments and prospects

A Sandwich Structure Sensor Based on Ag-Silk Fibroin-Ag Co-Modification for Trace Water Determination in Visible Light Band

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