Protein Detecting Arrays Based on Cationic Polythiophene–DNA‐Aptamer Complexes

Abérem, M. Béra; Najari, Ahmed; Ho, H.‐A.; Gravel, Jean-François; Nobert, Philippe; Boudreau, Denis; Leclerc, Mario

doi:10.1002/adma.200601651

Cited by 110 publications

(27 citation statements)

References 18 publications

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“…34,66 Compared with solid-state sensors based on the CP-dye FRET system, it showed merits of more simple, faster and low-cost since tedious operation (centrifugation, washing, drying, and ultrasonic operation) and long assay time were required for the solid-state detection. 66,75 In addition, our strategy also shows merits of fast, low-cost, label-free, and sensitive compared with fluorometric methods based on QDs-dye FRET system because the conjugation of biomolecules on the surface of QDs needs long time (several hours ) and labeled DNAs are much expensive than unlabeled ones. [67][68][69] Thus,…”

Section: ■ Results and Discussionmentioning

confidence: 98%

Thioflavin T as an Efficient G-Quadruplex Inducer for the Highly Sensitive Detection of Thrombin Using a New Föster Resonance Energy Transfer System

Liu

Hua

Fan

et al. 2015

ACS Appl. Mater. Interfaces

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We report a new Föster resonance energy transfer (FRET) system that uses a special dye, thioflavin T (ThT), as an energy acceptor and a water-soluble conjugated polymer (CP) with high fluorescence as an energy donor. A simple, label-free, and sensitive strategy for the detection of thrombin in buffer and in diluted serum was designed based on this new system using ThT as an efficient inducer of the G-quadruplex. The difference between the blank and the positive samples was amplified due to distinctive FRET signals because thrombin has little effect on the intercalation of ThT into the G-quadruplex. In the absence of the target, ThT induces the aptamer to form a G-quadruplex and intercalates into it with strong fluorescence. The electrostatic attractions between the negatively charged G-quadruplex and positively charged CP allow a short donor-acceptor distance, resulting in a high FRET signal. However, in the presence of the target, the aptamer forms a G-quadruplex-thrombin complex first, followed by the intercalation of ThT into the G-quadruplex. A long distance exists between the donor and acceptor due to the strong steric hindrance from the large-sized thrombin, which leads to a low FRET signal. Compared with previously reported strategies based on the FRET between the CP and dye, our strategy is label-free, and the sensitivity was improved by an order of magnitude. Our strategy also shows the advantages of being simple, rapid (about 50 min), sensitive, label-free, and low-cost in comparison to strategies based on the FRET between quantum dots and dyes.

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Section: ■ Results and Discussionmentioning

confidence: 98%

Thioflavin T as an Efficient G-Quadruplex Inducer for the Highly Sensitive Detection of Thrombin Using a New Föster Resonance Energy Transfer System

Liu

Hua

Fan

et al. 2015

ACS Appl. Mater. Interfaces

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“…A conjugated polyelectrolyte (CPE)1, 2 a conjugated polymer containing a charged (anionic or cationic) group, has received considerable attention for its bio‐applications such as solution‐based DNA sensor,3–11 DNA microarray,12–14 protein sensor,15–24 bioimaging25–28 as well as optoelectronic applications such as organic semiconductors,29–31 light‐emitting devices,32, 33 and actuators 34. The ionic side group plays an important role to provide CPEs with water‐solubility that is central to many biological applications.…”

Section: Introductionmentioning

confidence: 99%

Design Principle of Conjugated Polyelectrolytes to Make Them Water‐Soluble and Highly Emissive

Lee

Kim

2012

Adv Funct Materials

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The correlation between the molecular design of a conjugated polyelectrolyte (CPE) and its aggregated structure and the emissive properties in water is systematically investigated by means of UV–vis spectrometry, fluorescence spectroscopy, and scanning/transmission electron microscopy. Five different and rationally designed CPEs having carboxylic acid side chains are synthesized. All five conjugated polyelectrolytes are seemingly completely soluble in water in visual observation. However, their quantum yields are dramatically different, changing from 0.45 to 51.4%. Morphological analysis by electron microscopy combined with fluorescence spectrophotometry reveals that the CPEs form self‐assembled aggregates at the nanoscale depending on the nature of their side chains. The feature of the self‐assembled aggregates directly determines the emissive property of the CPEs. The nature and the length of the spacer between the carboxylic acid group and the CPE backbone have a strong influence on the quantum yield of the CPEs. Our study demonstrates that bulky and hydrophilic side chains and spacers are required to achieve complete water‐solubility and high quantum yield of CPEs in water, providing an important molecular design principle to develop functional CPEs.

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“…The advantages of CPs mean that they can be employed in protein detection with a label‐free manner. More importantly, highly charged CPs can be easily assembled on solid platforms to meet the demands of high‐throughput assay, which has been developed as a novel orientation for protein detection . However, relying on their electrostatic interactions with protein, it is difficult to obtain a response of fluorescent signal to the target protein binding events.…”

Section: Methodsmentioning

confidence: 99%

An Optical Nanoruler Based on a Conjugated Polymer−Silver Nanoprism Pair for Label‐Free Protein Detection

Wang

et al. 2015

Advanced Materials

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An optical nanoruler system based on a conjugated polyelectrolyte-silver nanoprism pair is developed for label-free protein detection by taking advantage of the metal-enhanced fluorescence effect of silver nanostructures. Antibody-antigen interactions induce a change in the metal-fluorophore distance, followed by the response of a fluorescent signal of the conjugated polyelectrolyte. The system is used to detect target antigens sensitively and selectively.

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Protein Detecting Arrays Based on Cationic Polythiophene–DNA‐Aptamer Complexes

Cited by 110 publications

References 18 publications

Thioflavin T as an Efficient G-Quadruplex Inducer for the Highly Sensitive Detection of Thrombin Using a New Föster Resonance Energy Transfer System

Thioflavin T as an Efficient G-Quadruplex Inducer for the Highly Sensitive Detection of Thrombin Using a New Föster Resonance Energy Transfer System

Design Principle of Conjugated Polyelectrolytes to Make Them Water‐Soluble and Highly Emissive

An Optical Nanoruler Based on a Conjugated Polymer−Silver Nanoprism Pair for Label‐Free Protein Detection

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