Protein Crystallization Using a High-Repetitive Low-Power Laser Beam Under Tightly Focused Condition

Watanabe, Shuichi; Nagasaka, S.; Noda, Kohki; Tashiro, Hideo

doi:10.1143/jjap.43.l941

Cited by 5 publications

(5 citation statements)

References 4 publications

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“…83,84 A similar experimental result was also reported by Watanabe et al 91 Lysozyme crystallization is not difficult, but more efficient crystallization giving better quality compared to the conventional method was demonstrated. We considered that the bubbling induced by multiphoton laser ablation is responsible to lysozyme crystallization.…”

Section: Crystallization Of Molecules and Proteins By Femtosecond Lassupporting

confidence: 55%

Time-Resolved Spectroscopic and Imaging Studies on Laser Ablation of Molecular Systems: From Mechanistic Study to Bio/Nano Applications

Masuhara

2013

Bulletin of the Chemical Society of Japan

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Studies on laser ablation of polymer films, molecular crystals in solution, protein solution, and culture media containing living cells are summarized and considered. Dynamics and mechanism of laser ablation were systematically studied by utilizing time-resolved spectroscopy and imaging; femtosecondnanosecond transient absorption and emission spectroscopy, nanosecond shadowgraphy, nanosecondnanometer interferometry, femtosecond surface light scattering imaging. It was confirmed by integrating both data that primary processes of laser ablation can be well understood in the framework of Jablonski diagram. For nanosecond laser ablation of doped polymers, it was demonstrated that cyclic multiphoton absorption is an efficient photothermal conversion process leading to photothermal ablation. For femtosecond laser ablation of dye films, transient pressure mechanism was proposed indicating photomechanical ablation. As applications of laser ablation, nanoparticle preparation, protein crystallization, and manipulation of living cells are presented. Laser ablation of molecular crystals in poor solvent gives small fragments whose size are in a few tens nm. The fabricated nanocolloids are stable without adding detergents and their size was the smallest as nanoparticles produced by the top-down-method. Multiphoton laser ablation of water generates local impulsive force due to bubble formation, shockwave propagation, and local convection flow. The force triggers molecular and protein crystallization in their supersaturated solutions, whose mechanisms are described and considered. The impulsive force is also very useful for manipulating living cells and its high potential was confirmed by examining cell functions such as division, differentiation, death, and migration. Finally summary and future plan are presented.Studies on molecular spectroscopy and photochemistry introduced lasers very early in 1960's and used them as light sources for spectroscopic measurements as well as for inducing photochemical reactions. When chemists started to use pulsed lasers, they soon became aware that laser irradiation induced vaporization, decomposition, and fragmentation of materials and found that the irradiated surface was etched. The left patterns on the colored papers were practically used to confirm optical alignment in experimental setups before infrared (IR) viewer became popular. Later this laser-induced phenomenon was called laser ablation by Srinivasan, 1 and its systematic application was quickly developed to fabricate microstructures of various materials by electronics engineers and to microsurgery by medical doctors. Laser ablation is made possible by applying pulsed lasers and never brought out by weak continuous wave (CW) laser even when its irradiation continues for 1 year. Laser ablation is one of representative nonlinear photochemical phenomena and has a threshold with respect with laser fluence. Initially most of laser ablation studies were carried out for metals, semiconductors, ceramics, and glasses, 2 while organic materi...

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Section: Crystallization Of Molecules and Proteins By Femtosecond Lassupporting

confidence: 55%

Time-Resolved Spectroscopic and Imaging Studies on Laser Ablation of Molecular Systems: From Mechanistic Study to Bio/Nano Applications

Masuhara

2013

Bulletin of the Chemical Society of Japan

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“…Light irradiation has been expected to be an innovative technique for the enforced nucleation. It has been reported that nucleation from a solution can be accelerated by a flash lamp or laser irradiation. − Nucleation will be induced by the effect of the electric field, photochemical reaction, laser-induced shock wave, and so on. In previous work, we applied a nanosecond infrared laser (Nd:YAG laser, 1064 nm, 23 ns) to a supersaturated methanol solution of DAST and succeeded in triggering nucleus generation at low supersaturation, in which nucleation does not occur without laser irradiation .…”

Section: Introductionmentioning

confidence: 99%

Femtosecond Laser-Induced Crystallization of 4-(Dimethylamino)-N-methyl-4-stilbazolium Tosylate

Hosokawa

Adachi

Yoshimura

et al. 2005

Crystal Growth & Design

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A femtosecond laser pulse was irradiated to a supersaturated methanol solution of an organic molecule, 4-(dimethylamino)-N-methyl-4-stilbazolium tosylate (DAST), and its efficient crystallization was demonstrated under concentration and temperature conditions where spontaneous crystallization does not occur. The efficiency was higher than that using a nanosecond laser, and the nucleation depended on the laser repetition rate. The results suggest that the nucleation is trigged by cluster formation, which is ascribed to femtosecond laser irradiation effects, such as shock wave generation due to the ablation of the medium and an optical pressure due to the strong electric field.

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“…Femtosecond (fs) laser ablation due to the multiphoton absorption of target materials under exposure of amplified fs pulses is considered to be a photomechanical phenomenon that can remove tiny part of the target material from its surface or generate physical perturbations to its surroundings without heat accumulation. − Besides, because the wavelength of a fs laser is tunable in the near-infrared window, fs laser light can penetrate biological tissues and cells efficiently. , Accordingly, fs laser ablation has been widely extended to various applications and research fields, ranging from material science on the scale of nanometers such as nanoparticle preparation and nanoparticle patterning − to crystallization and crystal growth on the scale of micrometers − to cell manipulation − and so on.…”

Section: Introductionmentioning

confidence: 99%

Femtosecond-Laser-Enhanced Amyloid Fibril Formation of Insulin

et al. 2017

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Femtosecond (fs)-laser-induced crystallization as a novel crystallization technique was proposed for the first time by our group, where the crystallization time can be significantly shortened under fs laser irradiation. Similarly, we have further extended our investigation to amyloid fibril formation, also known as a nucleation-dependence process. Here we demonstrate that the necessary time for amyloid fibril formation can be significantly shortened by fs laser irradiation, leading to favorable enhancement. The enhancement was confirmed by both spectral measurements and direct observations of amyloid fibrils. The thioflavin T fluorescence intensity of laser-irradiated solution increased earlier than that of the control solution, and such a difference was simultaneously revealed by ellipticity changes. At the same time before intensity saturation in fluorescence, the number of amyloid fibrils obtained under laser irradiation was generally more than that in the control solution. Besides, such an enhancement is correlated to the laser power threshold of cavitation bubbling. Possible mechanisms are proposed by referring to fs-laser-induced crystallization and ultrasonication-induced amyloid fibril formation.

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Protein Crystallization Using a High-Repetitive Low-Power Laser Beam Under Tightly Focused Condition

Cited by 5 publications

References 4 publications

Time-Resolved Spectroscopic and Imaging Studies on Laser Ablation of Molecular Systems: From Mechanistic Study to Bio/Nano Applications

Time-Resolved Spectroscopic and Imaging Studies on Laser Ablation of Molecular Systems: From Mechanistic Study to Bio/Nano Applications

Femtosecond Laser-Induced Crystallization of 4-(Dimethylamino)-N-methyl-4-stilbazolium Tosylate

Femtosecond-Laser-Enhanced Amyloid Fibril Formation of Insulin

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