Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles

Spagnoletti, Federico N.; Kronberg, Florencia; Spedalieri, Cecilia; Munarriz, Eliana; Giacometti, Romina

doi:10.1016/j.jenvman.2021.113434

Cited by 31 publications

(17 citation statements)

References 51 publications

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“…The obtained results of in vitro and in planta experiments are in general agreement and much promising when correlated with the previously reported studies on antimicrobial activities of silver nanoparticles ( Jo et al, 2009 ; Ali et al, 2015 ; Gopinath et al, 2017 ; Some et al, 2018 ; Alam et al, 2019 ; Haroon et al, 2019 ; Santiago et al, 2019 ; Vanti et al, 2019 ; Renuka et al, 2020 ; Rizwan et al, 2020 ; Tariq et al, 2021 ). The green AgNPs in the presence of plant capping agents did not allow particles to aggregate and increases its long-term stability ( Spagnoletti et al, 2021 ). The plant extract carries out dual function i.e., perform the reduction of silver ion and stabilization of AgNPs in the reaction mixture ( Sunkar and Nachiyar, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Biosynthesized silver nanoparticles using Polygonatum geminiflorum efficiently control fusarium wilt disease of tomato

Ahmad¹,

Ali²,

Ullah³

et al. 2022

Front. Bioeng. Biotechnol.

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Nanomaterials are gaining tremendous potential as emerging antimicrobials in the quest to find resistance-free alternatives of chemical pesticides. In this study, stable silver nanoparticles were synthesized using the aqueous extract of medicinal plant species Polygonatum geminiflorum, and their morphological features were evaluated by transmission electron microscopy, X-ray diffraction spectroscopy and energy dispersive X-ray analysis. In vitro Antifungal activity of the synthesized silver nanoparticles (AgNPs) and P. geminiflorum extract (PE) either alone or in combination (PE-AgNPs) against Fusarium oxysporum was evaluated using disc-diffusion and well-diffusion methods. In planta assay of the same treatments against Fusarium wilt diseases of tomato was evaluated by foliar spray method. Moreover, plant extract was evaluated for the quantitative investigation of antioxidant activity, phenolics and flavonoids by spectroscopic and HPLC techniques. Phytochemical analysis indicated the presence of total phenolic and flavonoid contents as 48.32 mg ± 1.54 mg GAE/g and 57.08 mg ± 1.36 mg QE/g, respectively. The DPPH radical scavenging of leaf extract was found to be 88.23% ± 0.87%. Besides, the HPLC phenolic profile showed the presence of 15 bioactive phenolic compounds. Characterization of nanoparticles revealed the size ranging from 8 nm to 34 nm with average crystallite size of 27 nm. The FTIR analysis revealed important functional groups that were responsible for the reduction and stabilization of AgNPs. In the in vitro assays, 100 μg/ml of AgNPs and AgNPs-PE strongly inhibited Fusarium oxysporum. The same treatments tested against Fusarium sprayed on tomato plants in controlled environment exhibited nearly 100% plant survival with no observable phytotoxicity. These finding provide a simple baseline to control Fusarium wilt using silver nano bio-control agents without affecting the crop health.

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Section: Discussionmentioning

confidence: 99%

Biosynthesized silver nanoparticles using Polygonatum geminiflorum efficiently control fusarium wilt disease of tomato

Ahmad¹,

Ali²,

Ullah³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Surface properties could also be changed by surface interactions, as shown above, involving the adsorbtion of proteins, lipids or other macromolecules (natural organic matter) at the surface of nanoparticles [33]. The formation of protein or lipid coronae at the surface of nAg was shown to preserve the Ag core, thereby limiting rapid diffusion of ionic Ag + into the medium and toxicity [38]. Serum bovine albumin binds at the surface of nAg, forming a corona which, in turn, could interact with lipid vesicles [39].…”

Section: Discussionmentioning

confidence: 99%

The Influence of Silver Nanoparticle Form on the Toxicity in Freshwater Mussels

et al. 2022

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The contribution of the form of silver nanomaterials (nAg) towards toxicity in aquatic organisms is not well understood. The purpose of this study was to examine the toxicity of various structures (sphere, cube and prism) of nAg in Dreissena bugensis mussels. Mussels were exposed to increasing concentrations of polyvinyl-coated nAg of the same size for 96 h at 15 °C. They were then analyzed for biophysical changes in the cytoplasm (viscosity, protein aggregation and lipids), neuro-activity (fractal kinetics of acetylcholinesterase (AChE)), oxidative stress (labile zinc (Zn) and lipid peroxidation) and inflammation (arachidonate cyclooxygenase). Although some decreasing effects in protein aggregation were observed, viscosity was more strongly decreased in mussels exposed to spheric and prismatic nAg. The activity of AChE was significantly decreased in the following form-dependent manner: prismatic > cubic > spheric nAg. The fractal dimension of AChE reactions was reduced by all geometries of nAg, while dissolved Ag had no effects. For nanoparticles with the same coating and relative size, spheric nAg produced more significant changes towards the fractal dimension of AChE, while prismatic nAg increased both protein aggregation and viscosity, whereas cubic nAg decreased protein aggregation in the cytoplasm. It is concluded that the geometries of nanoparticles could influence toxicity in aquatic organisms.

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“…Amooaghaie et al proved that cytotoxicity of the green synthesized AgNPs (using leaf extract of Nigella sativa ) was significantly less than wet-chemistry synthesized ones (using sodium borohydride and sodium citrate) [69] . AgNPs synthesized using cell-free filtrates from the fungus displayed less toxicity than chemical AgNPs in the model organism Caenorhabditis elegans , which is due to the proteins capping on the surface of bio-AgNPs [70] . Sur et al [71] investigated the cytotoxicity of naked AgNPs and AgNPs modified with glucose, lactose, and oligonucleotides on A549 cells, and the result showed that only naked AgNPs influence the viability of the A549 cells.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of silver nanoparticles using Eucommia ulmoides extract and their potential biological function in cosmetics

Kan

Zhu

et al. 2022

Heliyon

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Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles

Cited by 31 publications

References 51 publications

Biosynthesized silver nanoparticles using Polygonatum geminiflorum efficiently control fusarium wilt disease of tomato

Biosynthesized silver nanoparticles using Polygonatum geminiflorum efficiently control fusarium wilt disease of tomato

The Influence of Silver Nanoparticle Form on the Toxicity in Freshwater Mussels

Synthesis of silver nanoparticles using Eucommia ulmoides extract and their potential biological function in cosmetics

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