Protein corona of metal-organic framework nanoparticals: Study on the adsorption behavior of protein and cell interaction

Sun, Qiaomei; Zhao, Ludan; Tang, Peixiao; Suo, Zili; Zhang, Shuangshuang; Zhang, Yongkui; Zhang, Man; Wang, Wenjing; Li, Hui

doi:10.1016/j.ijbiomac.2019.08.183

Cited by 35 publications

(19 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Peaks locating at 1673 or 1543 cm −1 of HSA possessed an obvious shifts with the addition of Co‐cmtna , as well as the significant changes in the fingerprint (dotted box), which showed the existence of the interactions between analyst and HSA. [ 32,33 ] The similar conditions also occurred when Hcmtna was added into HSA solution. In addition, TGA experiments (Figure S29) also confirmed that there are new complexes after the interaction between analytes with HSA.…”

Section: Resultsmentioning

confidence: 87%

“…The helicity of HSA decreases with the addition of two analytes, which means that the analytes bind to the amino acid residues of the HSA polypeptide backbone, destroy the hydrogen bond structure of HSA, stretch the peptide chain structure of HSA, and change the secondary structure. [ 32,37 ] The CD spectra of HSA before and after the addition of the analyte are similar in shape, indicating that the secondary structure of HSA is still dominated by α‐helix (Table S6).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A multiple fluorescence sensor with the sensitive recognition to human serum albumin

Xing

Gao

et al. 2021

Applied Organom Chemis

View full text Add to dashboard Cite

A naphthalene-containing dicarboxylic ligand, 4-(carboxymethoxy)-6-methyl-1-(p-tolyl)-2-naphthoic acid (Hcmtna), is synthesized and used to construct a coordination compound, Co(H 2 O) 4 (cmtna) 2 (Co-cmtna), through the hydrothermal strategy. The moderate rigidity and flexibility of the organic molecule result a low coordination number of cobalt ion with the ligand and provide more possibilities for potential applications. Experimental results show that the fluorescence of Co-cmtna can be quenched by multiple objects, including antibiotics, pesticides, and metal ions, which make it possible as a multifunctional sensor. More interestingly, Co-cmtna has interactions with human serum albumin in the phosphate-buffered saline solution, which are detailed studied by the site binding model formula, thermodynamic formula, and Föster nonradiative energy transfer mechanism.

show abstract

Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

A multiple fluorescence sensor with the sensitive recognition to human serum albumin

Xing

Gao

et al. 2021

Applied Organom Chemis

View full text Add to dashboard Cite

show abstract

“…Superparamagnetic iron oxide nanoparticles, which are clinically employed for resonance imaging of atherosclerotic plaques [37], when tested in vitro, engaged CR3 as delineated using CD11b-blocking antibodies [38]. However, it remains unclear whether, under these circumstances, constituents of the protein corona, which is inevitably formed by contact with FCS in cell culture media [39], may have served as CR3 ligand(s).…”

Section: Discussionmentioning

confidence: 99%

Complement-Opsonized Nano-Carriers Are Bound by Dendritic Cells (DC) via Complement Receptor (CR)3, and by B Cell Subpopulations via CR-1/2, and Affect the Activation of DC and B-1 Cells

Bednarczyk

Medina‐Montano

Fittler

et al. 2021

IJMS

View full text Add to dashboard Cite

The development of nanocarriers (NC) for biomedical applications has gained large interest due to their potential to co-deliver drugs in a cell-type-targeting manner. However, depending on their surface characteristics, NC accumulate serum factors, termed protein corona, which may affect their cellular binding. We have previously shown that NC coated with carbohydrates to enable biocompatibility triggered the lectin-dependent complement pathway, resulting in enhanced binding to B cells via complement receptor (CR)1/2. Here we show that such NC also engaged all types of splenic leukocytes known to express CR3 at a high rate when NC were pre-incubated with native mouse serum resulting in complement opsonization. By focusing on dendritic cells (DC) as an important antigen-presenting cell type, we show that CR3 was essential for binding/uptake of complement-opsonized NC, whereas CR4, which in mouse is specifically expressed by DC, played no role. Further, a minor B cell subpopulation (B-1), which is important for first-line pathogen responses, and co-expressed CR1/2 and CR3, in general, engaged NC to a much higher extent than normal B cells. Here, we identified CR-1/2 as necessary for binding of complement-opsonized NC, whereas CR3 was dispensable. Interestingly, the binding of complement-opsonized NC to both DC and B-1 cells affected the expression of activation markers. Our findings may have important implications for the design of nano-vaccines against infectious diseases, which codeliver pathogen-specific protein antigen and adjuvant, aimed to induce a broad adaptive cellular and humoral immune response by inducing cytotoxic T lymphocytes that kill infected cells and pathogen-neutralizing antibodies, respectively. Decoration of nano-vaccines either with carbohydrates to trigger complement activation in vivo or with active complement may result in concomitant targeting of DC and B cells and thereby may strongly enhance the extent of dual cellular/humoral immune responses.

show abstract

“…Due to their chemical and physical properties metal NPs interact with extracellular molecules. In the body NPs form so-called surface corona consisting of extracellular proteins [15]. Circulating NPs collect immunomodulatory proteins including the components of the complement system, hemoglobin, immunoglobulins, as well as cell growth/differentiation factors [16][17][18].…”

Section: Non-specific Interactions With Extracellular Proteinsmentioning

confidence: 99%

Metal Nanoparticles in Immunotherapy: Applications, Limitations and Pespectives

Dukhinova¹,

Shestovskaya

2020

RDMS

View full text Add to dashboard Cite

Metal nanoparticles and immune cell heterogeneityCurrently, various metal NPs are used for diagnostics and therapy. The metal component can be represented by Ag, Au, Pt, rare earth metals, oxides of iron, nickel, copper, and others. These NPs can interact with the immune cells depending on the delivery root (local or systemic, intravenous or dietary or intranasal) [1,2]. Metal nanoparticles and inborn immunityOnce NPs enter the body, they encounter circulating monocytes or tissue resident macrophages that actively engulf and transfer NPs to different sites. NPs can polarize macrophages towards pro-(M1) or anti-inflammatory (M2) profile with respect to microenvironment and the type of metal [3]. M1 and M2 macrophages release a variety of cytokines which, in turn, recruit other immune cells that suppress (regulatory T cells) or prolong (cytotoxic T cells, T helpers, B lymphocytes) inflammation and the immune reaction triggered by NPs. Simultaneously with macrophages, NPs target neutrophils, the innate immune cells that fight against pathogens via extracellular trap formation and subsequent specific cell death termed netoptosis, for pathogen restriction and killing. A number of metal NPs up-regulate neutrophil extracellular trap activity and stimulate antimicrobial potential [4,5]. In some cases, nanosilver can also cause non-classical activation of neutrophils with IL8 release but not netoptosis, and the effect depends on NP size [6,7].

show abstract

Protein corona of metal-organic framework nanoparticals: Study on the adsorption behavior of protein and cell interaction

Cited by 35 publications

References 38 publications

A multiple fluorescence sensor with the sensitive recognition to human serum albumin

A multiple fluorescence sensor with the sensitive recognition to human serum albumin

Complement-Opsonized Nano-Carriers Are Bound by Dendritic Cells (DC) via Complement Receptor (CR)3, and by B Cell Subpopulations via CR-1/2, and Affect the Activation of DC and B-1 Cells

Metal Nanoparticles in Immunotherapy: Applications, Limitations and Pespectives

Contact Info

Product

Resources

About