Protein corona formation of human serum albumin with carbon quantum dots from roast salmon

Song, Yukun; Wang, Haitao; Zhang, Lijuan; Lai, Bin; Liu, Kangjing; Tan, Mingqian

doi:10.1039/c9fo02967b

Cited by 31 publications

(23 citation statements)

References 47 publications

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“…Additionally, MnCQD presents very selective affinity to BSA when compared with hTF ( Fig. 3d), or even when compared with similar CQD binding investigation with other proteins reported in the literature [37][38][39] .…”

Section: Affinities and Thermodynamic Aspects Of The Interactionssupporting

confidence: 78%

“…It reveals protein structure stability around the fluorescent residues, e.g., more concretely sites I and II, during BSA-MnCQD interaction. Furthermore, a recent reporter investigated the displacement of the well-known site ligand (warfarin and ibuprofen) and identified sites I and II of human serum albumin as the anchoring region for the CQD nanoparticles 38 . HTF experiences a visible shift on the λ max =338nm to 345nm (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Non-passivated manganese-based carbonaceous nanoparticles selectively target kidney outer medulla, exhibiting renal clearance, high T1-weighted MRI contrast, subtle citotoxicity and low opsonization level.

Garcia¹,

Menezes

Barros

et al. 2021

Preprint

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Nanoparticle passivation by molecular recognition moieties (small molecules, aptamers, antibodies) imputes unique characteristics to nanoparticles (NPs) and unlocks their fascinating selectivity to targeting disease sites. Although non-passivated nanomaterials may archive selective targeting by exploring NP features and their interaction with biofluids proteins, such studies are scarce in the literature. Here, we report spherical manganese-based carbonaceous NPs (MnCQD), which specifically target mice kidneys after intravenous injection, even without direct surface chemical modification. Also, the NPs provide high image contrast in T1 magnetic resonance imaging (MRI) with subtle toxicological effects. The unexpected selectivity of MnCQD to the kidney has been examined based on their determined intrinsic properties and their interaction with two blood proteins: Bovine Serum Albumin (BSA), and Human Transferrin (HTF). More particularly, aspects such as size, composition, superficial charge, spectroscopic features, interaction mechanism, affinities, thermodynamic, and protein structural changes have been investigated. All these results highlight the excellent potential of MnCQD as a low toxic T1-MRI contrast agent and open the prospect of using non-functionalized NPs as a selective agent to target specific organs.

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Section: Affinities and Thermodynamic Aspects Of The Interactionssupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Non-passivated manganese-based carbonaceous nanoparticles selectively target kidney outer medulla, exhibiting renal clearance, high T1-weighted MRI contrast, subtle citotoxicity and low opsonization level.

Garcia¹,

Menezes

Barros

et al. 2021

Preprint

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show abstract

“…It has been reported that the FCDs derived from grilled salmon could combine with digestive proteases (pepsin, trypsin) through the static interaction mechanism, change the secondary structure of the two proteases and inhibit enzyme activity [5]. Human serum albumin (HSA) could reduce the cytotoxicity of FCDs in grilled salmon, reduce the rate Foods 2021, 10, 2336 2 of 15 of apoptosis and slow down the disorders of glucose and lipid metabolism [7]. Moreover, the FCDs from roast beef could combine with HSA through electrostatic interaction or hydrophobic force, and were distributed in cell lysosomes.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Carbon Dots from Grilled Spanish Mackerel with Human Serum Albumin, γ-Globulin and Fibrinogen

Cui

Song

Liu

et al. 2021

Foods

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The potential biological effects of food-borne carbon dots (FCDs) generated during food heating procedures on human health has received great attention. The FCDs will be inevitably exposed to blood proteins along with our daily diet to produce unknown biological effects. In this study, the interaction between FCDs extracted from grilled Spanish mackerel and three main types of human plasma proteins including human serum albumin (HSA), human γ-globulin (HGG) and human fibrinogen (HF) was reported. It was found that the grilled Spanish mackerel FCDs could affect the morphology, size and surface electrical properties of the three proteins. The interaction between the FCDs and proteins had different effects on the secondary structure of the three proteins through a static mechanism. The tested HSA, HGG, and HF could adsorb FCDs to reach saturation state within 0.5 min after the adsorption happened. The binding affinity of the FCDs to the plasma proteins was sorted as follows: HF > HGG > HSA. The results of FCDs interacted with plasma proteins provided useful information in the assessment of the safety of FCDs in our daily diet.

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“…To confirm N-CQDs’ cytocompatibility and phenomena of their lowered oxidative toxicity, we have focused on their interactions within the cellular microenvironment, specifically on the interplay with proteins. The protein corona formation of CQDs from roast salmon with serum albumin has only recently been reported and assumed to reduce the toxicity of quantum dots [ 37 ]. The bovine serum albumin coating strongly decreases their oxidative properties and does not quench their intrinsic fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Protein Corona Hinders N-CQDs Oxidative Potential and Favors Their Application as Nanobiocatalytic System

Czarnecka

Kwiatkowski

Wiśniewski

et al. 2021

IJMS

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The oxidative properties of nanomaterials arouse legitimate concerns about oxidative damage in biological systems. On the other hand, the undisputable benefits of nanomaterials promote them for biomedical applications; thus, the strategies to reduce oxidative potential are urgently needed. We aimed at analysis of nitrogen-containing carbon quantum dots (N-CQDs) in terms of their biocompatibility and internalization by different cells. Surprisingly, N-CQD uptake does not contribute to the increased oxidative stress inside cells and lacks cytotoxic influence even at high concentrations, primarily through protein corona formation. We proved experimentally that the protein coating effectively limits the oxidative capacity of N-CQDs. Thus, N-CQDs served as an immobilization support for three different enzymes with the potential to be used as therapeutics. Various kinetic parameters of immobilized enzymes were analyzed. Regardless of the enzyme structure and type of reaction catalyzed, adsorption on the nanocarrier resulted in increased catalytic efficiency. The enzymatic-protein-to-nanomaterial ratio is the pivotal factor determining the course of kinetic parameter changes that can be tailored for enzyme application. We conclude that the above properties of N-CQDs make them an ideal support for enzymatic drugs required for multiple biomedical applications, including personalized medical therapies.

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Protein corona formation of human serum albumin with carbon quantum dots from roast salmon

Abstract: Carbon quantum dots (CQDs) extracted from roast salmon could interact with human serum albumin (HSA) to form protein coronas, changing their biological identity.

Cited by 31 publications

References 47 publications

Non-passivated manganese-based carbonaceous nanoparticles selectively target kidney outer medulla, exhibiting renal clearance, high T1-weighted MRI contrast, subtle citotoxicity and low opsonization level.

Non-passivated manganese-based carbonaceous nanoparticles selectively target kidney outer medulla, exhibiting renal clearance, high T1-weighted MRI contrast, subtle citotoxicity and low opsonization level.

Interaction of Carbon Dots from Grilled Spanish Mackerel with Human Serum Albumin, γ-Globulin and Fibrinogen

Protein Corona Hinders N-CQDs Oxidative Potential and Favors Their Application as Nanobiocatalytic System

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