Protein corona fingerprinting to differentiate sepsis from non-infectious systemic inflammation

Abstract

Exploitation of the nanoparticle protein corona to identify proteomic fingerprints in blood that differentiate non-bacterial systemic inflammation from sepsis.

The biomolecule corona of lipid nanoparticles contains circulating cell-free DNA

“…Our group has previously detected histone proteins in human and mouse liposomal corona, both ex vivo and in vivo. 9,11,12 Moreover, human histone proteins (H2B and H4) have also been found to interact with colloidal gold NPs upon incubation with human plasma. 54 Finally, De Paoli and colleagues demonstrated that calf thymus histone H1 can bind onto carboxylated-multiwalled carbon nanotubes.…”

“…PEGylated liposomes were found to reproducibly interact with low molecular weight (MW) plasma proteins of low abundance. [9][10][11][12]15 In order to assess the potential interaction of cfDNA with PEGylated liposomal surfaces, plasma-incubated liposomes were purified by size exclusion chromatography (SEC), shown in Fig. 1A, as described previously.…”

“…7 More recently, attempts have been made to purposefully manipulate the NP corona to modify the function of NPs 4,8 and this approach has since been exploited as an application in its own right. [9][10][11][12] For example, altering the surface properties of The aim of this study was to interrogate experimentally the fundamental question whether circulating cell-free DNA (cfDNA) exists in the biomolecule corona that spontaneously forms around nanoparticles upon incubation with complex biological fluids. Despite recent advances in the characterisation of the protein content of the biomolecule corona, to date there has been no experimental evidence of the spontaneous interaction between cfDNA and plasma-incubated nanoparticles.…”

“…4,6 In an alternative proposition, our laboratory has illustrated the potential exploitation of protein corona as a proteomic biomarker discovery platform that enables a higher-definition, in-depth analysis of the blood proteome and the enrichment of low abundant disease-specific molecules. [9][10][11][12] Despite the comprehensive characterisation of the bloodderived NP protein corona in mice and humans (both ex vivo and in vivo), 10,13,[15][16][17][18][19] little attention has been placed on other types of biomolecules that may constitute the biomolecule corona. Growing evidence suggests that lipids and metabolites also interact with the surface of NPs.…”

COVID-19: Nanomedicine Uncovers Blood-Clot Mystery

“…Many studies have identified this concept for several life-threatening diseases such as cancers and neurodegenerative diseases. 16 , 60 , 95 − 101 …”