Protein content and electrophoretic profile of fat body and ovary extracts from workers of Melipona quadrifasciata anthidioides (Hymenoptera, Meliponini)

Oliveira, Vagner Tadeu Paes de; Cruz-Landim, Carminda da

doi:10.1590/s0073-47212004000400010

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…Firstly, it is in close proximity to the heart, ostia, and body cavities. Secondly, according to Paes de Oliveira and Cruz-Landim 37 , it is in the trophocytes that vitellogenin is synthesized, the concentration of which is most probably the highest in the third tergite from where it is immediately directed to the appropriate tissues by the circulatory system. This solution enables a rapid distribution of these substances and ensures the queenְ’s reproductive dominance 59 .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, fat bodies accumulate toxic and reserve compounds (e.g. urea, uric acid) 37 and protect against oxidative stress, enhancing the apian resistance systems 38 – 41 . Roma et al 42 suggest that a few modifications had occurred during the evolution of the insects in the cells of the fat body, which resulted in the functional specialization of the individuals.…”

Section: Introductionmentioning

confidence: 99%

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Segmentation of the subcuticular fat body in Apis mellifera females with different reproductive potentials

Strachecka

Olszewski

Kuszewska

et al. 2021

Sci Rep

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Evolution has created different castes of females in eusocial haplodiploids. The difference between them lies in their functions and vulnerability but above all in their reproductive potentials. Honeybee queens are highly fertile. On the other hand, the workers are facultatively sterile. However, rebel workers, i.e. workers that develop in a queenless colony, reproduce more often than normal workers. As a result, the fat body of these bees, which apart from acting as the energy reserve, is also the site of numerous metabolic processes, had to specialize in different functions perfected over millions of years of eusocial evolution. Assuming that the variety of functions manifests itself in the pleomorphic structure of the fat body cells, we predicted that also different parts of the fat body, e.g. from different segments of the abdomen, contain different sets of cells. Such differences could be expected between queens, rebels and normal workers, i.e. females with dramatically different reproductive potentials. We confirmed all these expectations. Although all bees had the same types of cells, their proportion and segmental character corresponded with the caste reproductive potential and physiological characteristics shaped in the evolutionary process. The females with an increased reproductive potential were characterized by the presence of oenocytes in the third tergite and high concentrations of compounds responsible for energy reserves, like glucose, glycogen and triglycerides. Queens had very large trophocytes, especially in the third tergite. Only in workers did we observe intercellular spaces in all the segments of the fat body, as well as high protein concentrations—especially in the sternite. As expected, the rebels combined many features of the queens and normal workers, what with other findings can help understand the ways that led to the origin of different castes in females of eusocial Hymenoptera.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Segmentation of the subcuticular fat body in Apis mellifera females with different reproductive potentials

Strachecka

Olszewski

Kuszewska

et al. 2021

Sci Rep

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“…rufa along with senescence processes, in May and June. Cruz-Landim [ 39 ] and Paes de Oliveira and Cruz-Landim [ 31 ] confirmed that it was these proteins and other compounds, including antioxidants and proteases, that were synthetized in these specific cells.…”

Section: Discussionmentioning

confidence: 92%

“…Moreover, fat bodies accumulate toxic and reserve compounds [e.g. urea, uric acid; [ 31 ]] and protect against oxidative stress, enhancing the apian resistance systems, as well as having effect on overwintering and post-wintering performance [ 32 – 35 ]. They actively participate in vitellogenesis [ 36 – 38 ].…”

Section: Introductionmentioning

confidence: 99%

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Insights into the biochemical defence and methylation of the solitary bee Osmia rufa L: A foundation for examining eusociality development

et al. 2017

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We examined age-related biochemical and histological changes in the fat bodies and hemolymph of Osmia rufa males and females. We analysed solitary bees during diapause, in October and in April; as well as the flying insects following diapause, in May and June. The trophocyte sizes, as well as the numbers of lipid droplets were the greatest at the beginning of diapause. Subsequently, they decreased along with age. Triglyceride and glucose concentrations systematically decreased in fat body cells but increased in the hemolymph from October to June. Concentrations/activities of (enzymatic and non-enzymatic) antioxidant and proteolytic systems, as well as phenoloxidase, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase levels were constant during the diapause, usually lower in the males than the females. Prior to the diapause/overwintering, the concentrations/activities of all the compounds were higher in the fat bodies than in the hemolymph. Later in the spring and in the summer, they increased in the hemolymph and on the body surfaces, while decreasing in the fat bodies. The global DNA methylation levels increased with age. Higher levels were always observed in the males than in the females. The study will promote better understanding of bee evolution and will be useful for the protection and management of solitary bees, with benefits to the environment and agriculture.

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Cytochemistry of fat body trophocytes and ovaries of workers and queens of Melipona quadrifasciata anthidioides (Hymenoptera: Apidae: Meliponini) during vitellogenesis

Paes-de-Oliveira

Roat

Berger

et al. 2012

Microscopy Res & Technique

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The fat body (FB) of insects is where yolk proteins are synthesized. Therefore, relationships between the FB and oogenesis were studied in nurse workers, virgins, and physogastric queens of Melipona quadrifasciata anthidioides, a stingless bee in which the workers produce and lay eggs while provisioning brood cells. The relationships between FB and oogenesis, as well as the routes of materials from hemolymph to the oocytes, were studied through the cytochemical detection of lipids by osmium imidazole (OI), carbohydrates by ruthenium red (RR) and basic proteins by ammoniacal silver (AS). The results show differences in the presence of the studied materials in FB trophocytes and ovary of the classes of females studied and oogenesis phases. Material that tested positive for the treatments was detected among the classes of individuals studied in both, trophocytes and oocytes, and in the route of those materials from hemolymph to the oocytes. The differences found among the individual classes indicate relationships with the nutrition and adaptation to the parsimonious use of nutrients in the metabolism of reproduction.

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Protein content and electrophoretic profile of fat body and ovary extracts from workers of Melipona quadrifasciata anthidioides (Hymenoptera, Meliponini)

Cited by 11 publications

References 23 publications

Segmentation of the subcuticular fat body in Apis mellifera females with different reproductive potentials

Segmentation of the subcuticular fat body in Apis mellifera females with different reproductive potentials

Insights into the biochemical defence and methylation of the solitary bee Osmia rufa L: A foundation for examining eusociality development

Cytochemistry of fat body trophocytes and ovaries of workers and queens of Melipona quadrifasciata anthidioides (Hymenoptera: Apidae: Meliponini) during vitellogenesis

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