Protein Conformational Dynamics and Signaling in Evolution and Pathophysiology

Schweizer, Liang; Mueller, Luciano

doi:10.1016/b978-0-12-411460-9.00007-0

Cited by 7 publications

(16 citation statements)

References 239 publications

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“…The specific activity of the enzyme in the culture supernatant was even higher than that reported for the mesophilic enzyme from Bacillus sp. GL1 (1390 U/g) [17] despite the generally accepted belief that the rigidity of thermostable molecule limits enzyme activity [18]. Maximum enzyme productivity of 64.8 U/(L h) was observed at D = 0.1 h −1 .…”

Section: Gellan Lyase Produced By Geobacillus Stearothermophilus 98mentioning

confidence: 97%

“…2018, 18,[758][759][760][761][762][763][764][765][766][767] in cell culture and tissues; inert carriers in the manufacturing of drugs; or impregnates in the paper industry [13]. Life Sci.…”

Section: Gellan Lyase Produced By Geobacillus Stearothermophilus 98mentioning

confidence: 99%

“…This strain was able to grow and synthesize the enzyme in a pH range of 5.5-8.5 with an optimum at 7.5, the optimal temperature for synthesis was 55°C. GL1 (1390 U/g) [17] despite the generally accepted belief that the rigidity of thermostable molecule limits enzyme activity [18]. An activity of 79 U/L was reached in a mineral medium with 0.2% gellan in a laboratory reactor during the late exponential phase and the whole process continued only 15 h. In comparison, the fermentation processes of mesophilic gellan lyase producers last for several days.…”

Section: Gellan Lyase Produced By Geobacillus Stearothermophilus 98mentioning

confidence: 99%

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Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Kambourova

2018

Engineering in Life Sciences

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Thermostable enzymes (thermozymes) have been recognized as extremophilic compounds with a greatest biotechnological importance in different industrial areas. Quite recently exopolysaccharides (EPSs) synthesized by thermophiles became an object of increased research interest due to their unique properties appropriate for some specific industrial needs. Thermophilic producers of biotechnologically valuable enzymes and novel EPS were isolated by our group from Bulgarian thermal springs with a diverse geotectonic origin and different water properties. Laboratory reactor processes for their production were developed in batch and continuous cultures. Some of the synthesized thermostable enzymes were among the first described in their groups, for example, the single known thermostable gellan lyase that demonstrated specific activity higher than that of the mesophilic enzymes. Isolated by us thermostable xylanase was able to degrade more than 60% of beechwood xylan in a coprocess with an archaeal β‐xylosidase. Lipase purified by us was active between 55 and 90°C with an optimum at 75–80°C in a large pH range. It was able to degrade a broad range of substrates. Isolates from Bulgarian hot springs synthesized EPS with novel composition and high thermostability. Thus, Bulgarian hot springs harbor a wide set of thermophilic producers of novel enzymes and EPS with potential for a large number of biotechnological applications.

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Section: Gellan Lyase Produced By Geobacillus Stearothermophilus 98mentioning

confidence: 97%

“…2018, 18,[758][759][760][761][762][763][764][765][766][767] in cell culture and tissues; inert carriers in the manufacturing of drugs; or impregnates in the paper industry [13]. Life Sci.…”

Section: Gellan Lyase Produced By Geobacillus Stearothermophilus 98mentioning

confidence: 99%

Section: Gellan Lyase Produced By Geobacillus Stearothermophilus 98mentioning

confidence: 99%

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Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Kambourova

2018

Engineering in Life Sciences

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“…With respect to PD, a key molecule is brain α-synuclein, which is present at high levels and is concentrated in the presynaptic nerve terminals. It is involved in controlling the plasticity of dopamine overflow, synaptic vesicle recycling, storage, and the compartmentalization of neurotransmitters ( 165 ). In PD, α-synuclein aggregates into Lewy bodies, the abnormal intracellular inclusions that are the classical pathologic hallmark of the disease ( 166 ).…”

Section: Degenerative Brain Diseases Hb and Gh/igf-imentioning

confidence: 99%

Growth Hormone and Neuronal Hemoglobin in the Brain—Roles in Neuroprotection and Neurodegenerative Diseases

et al. 2021

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In recent years, evidence for hemoglobin (Hb) synthesis in both animal and human brains has been accumulating. While circulating Hb originating from cerebral hemorrhage or other conditions is toxic, there is also substantial production of neuronal Hb, which is influenced by conditions such as ischemia and regulated by growth hormone (GH), insulin-like growth factor-I (IGF-I), and other growth factors. In this review, we discuss the possible functions of circulating and brain Hb, mainly the neuronal form, with respect to the neuroprotective activities of GH and IGF-I against ischemia and neurodegenerative diseases. The molecular pathways that link Hb to the GH/IGF-I system are also reviewed, although the limited number of reports on this topic suggests a need for further studies. In summary, GH and/or IGF-I appear to be significant determinants of systemic and local brain Hb concentrations through mediating responses to oxygen and metabolic demand, as part of the neuroprotective effects exerted by GH and IGF-I. The nature and quantity of the latter deserve further exploration in specific experiments.

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“…Also, there is no structural information available till date. Based on the prediction by TMHMM server for transmembrane prediction, it is found that the N-terminal residues (1-11), middle region residues (91-112) and C-terminal residues (231-290) lies outside the membrane. Molecular Dynamics (MD) simulations showed that NSP6 consisting of helical structures, whereas membrane outside lying region (91-112) showed partial helicity, which further used as model and obtain disordered type conformation after 1.5 microsecond.…”

mentioning

confidence: 99%

Investigating the conformational dynamics of SARS-CoV-2 NSP6 protein with emphasis on non-transmembrane 91-112 & 231-290 regions

Kumar¹,

Kumar²,

Saumya³

et al. 2021

Preprint

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The NSP6 protein of SARS-CoV-2 is a transmembrane protein, with some regions lying outside the membrane. Besides, a brief role of NSP6 in autophagosome formation, this is not studied significantly. Also, there is no structural information available till date. Based on the prediction by TMHMM server for transmembrane prediction, it is found that the N-terminal residues (1-11), middle region residues (91-112) and C-terminal residues (231-290) lies outside the membrane. Molecular Dynamics (MD) simulations showed that NSP6 consisting of helical structures, whereas membrane outside lying region (91-112) showed partial helicity, which further used as model and obtain disordered type conformation after 1.5 microseconds. Whereas, the residues 231-290 has both helical and beta sheet conformations in its structure model. A 200ns simulations resulted in the loss of beta sheet structures, while helical regions remained intact. Further, we have characterized the residue 91-112 by using reductionist approaches. The NSP6 (91-112) was found disordered like in isolation, which gain helical conformation in different biological mimic environmental conditions. These studies can be helpful to study NSP6 (91-112) interactions with host proteins, where different protein conformation might play significant role. The present study adds up more information about NSP6 protein aspect, which could be exploited for its host protein interaction and pathogenesis.

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Protein Conformational Dynamics and Signaling in Evolution and Pathophysiology

Cited by 7 publications

References 239 publications

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Growth Hormone and Neuronal Hemoglobin in the Brain—Roles in Neuroprotection and Neurodegenerative Diseases

Investigating the conformational dynamics of SARS-CoV-2 NSP6 protein with emphasis on non-transmembrane 91-112 & 231-290 regions

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