Protein Concentration and Vigor of Imbibed Density-separated Primula Seed

Abstract

<jats:p>Seeds of <jats:italic>Primula acaulis</jats:italic> (L.) J. Hill `Dukaat Helderrose' were imbibed for 48 h at 18C, and Maltrin 600 solution was used to separate them into density classes of 1.10, 1.12, 1.14, 1.16, and 1.18 g·cm<jats:sup>-3</jats:sup>. Seeds were rinsed, air-dried for 48 h at 22C, and germinated at 18C; 80% were separated into density classes between 1.16 and 1.18 g·cm<jats:sup>-3</jats:sup>. Percentages of germination and high-vigor seedlings were significantly greater for seeds with densities &gt;1.14 g·cm<jats:sup>-3</jats:sup>. Soluble protein concentration increased with density, whereas insoluble protein concentration was unchanged. Distinct protein groups with estimated molecular weights of ≈50, 33, and 25 kDa were present in the protein profile of nonseparated imbibed seed. These proteins were most abundant at densities ≥1.16 g·cm<jats:sup>-3</jats:sup>. The 25-kDa protein was present only in higher density seed. Presence of an additional 14-kDa protein was noted in the insoluble protein profile. Certain proteins in the soluble protein fraction maybe used to test for seed vigor.</jats:p>

National proceedings: forest and conservation nursery associations—1995.

“…Therefore, viability and germination are often correlated with seed size and (or) density. Khademi et al (1993) showed that as the density of Primula acaulis (L.) rose, the percent viability and soluble protein contents increased. The densities of the seed ranged from 1.10 to 1.18 g/cm 3 .…”