Protein composition of <i>Methanococcus thermolithotrophicus</i> flagella

Kostyukova, Alla S.; Gongadze, Georgi M.; Obraztsova, Anna; Лауринавичус, К. С.; Fedorov, O.

doi:10.1139/m92-190

Cited by 12 publications

(8 citation statements)

References 10 publications

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“…The long flagellin genes (flaB1 Mt and flaB2 Mt ) share high sequence identity; however, flaB2 Mt appears to encode an internal 220-amino-acid (220-aa) sequence that is unique to this flagellin. A previous study reported three major proteins of 62, 44, and 26 kDa in M. thermolithotrophicus flagellar filaments (30). Our analysis of KBr gradient-purified M. thermolithotrophicus flagellar filaments resulted in the identification of proteins with apparent molecular masses of 62, 44, 37, and 26 kDa by SDS-PAGE (data not shown), while the flagellin genes are predicted to encode proteins with molecular masses of 46.2, 34.9, 22.9, and 22.7 kDa.…”

Section: Resultsmentioning

confidence: 90%

Characterization of Flagellum Gene Families of Methanogenic Archaea and Localization of Novel Flagellum Accessory Proteins

Thomas

Jarrell

2001

J Bacteriol

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Section: Resultsmentioning

confidence: 90%

Characterization of Flagellum Gene Families of Methanogenic Archaea and Localization of Novel Flagellum Accessory Proteins

Thomas

Jarrell

2001

J Bacteriol

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“…Cruden et al [20] reported that the integrity of £agella ¢laments from M. thermolithotrophicus was lost in the absence of high NaCl concentrations as well as when incubated at pH 11 for 1 h. However, Kostyukova et al [68] reported that ¢laments of M. thermolithotrophicus were still observed by electron microscopy after either of these treatments.…”

Section: Stabilitymentioning

confidence: 99%

The archaeal flagellum: a different kind of prokaryotic motility structure

Thomas

2001

FEMS Microbiology Reviews

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The archaeal flagellum is a unique motility apparatus distinct in composition and likely in assembly from the bacterial flagellum. Gene families comprised of multiple flagellin genes co-transcribed with a number of conserved, archaeal-specific accessory genes have been identified in several archaea. However, no homologues of any bacterial genes involved in flagella structure have yet been identified in any archaeon, including those archaea in which the complete genome sequence has been published. Archaeal flagellins possess a highly conserved hydrophobic N-terminal sequence that is similar to that of type IV pilins and clearly unlike that of bacterial flagellins. Also unlike bacterial flagellins but similar to type IV pilins, archaeal flagellins are initially synthesized with a short leader peptide that is cleaved by a membrane-located peptidase. With recent advances in genetic transfer systems in archaea, knockouts have been reported in several genes involved in flagellation in different archaea. In addition, techniques to isolate flagella with attached hook and anchoring structures have been developed. Analysis of these preparations is under way to identify minor structural components of archaeal flagella. This and the continued isolation and characterization of flagella mutants should lead to significant advances in our knowledge of the composition and assembly of archaeal flagella.

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“…The flagella of members of the Domain Archaea are not as well studied as their bacterial counterparts. Most of the studies of archaeal flagella and their component flagellins have been concentrated in the methanogens (Kalmokoff et al, 1988;Faguy et al, 1994b;Kostyukova et al, 1992) and extreme halophiles (Alam and Oesterhelt 1984;Gerl and Sumper, 1988;Fedorov et al, 1994). Nonetheless, many interesting features have been reported that seem to indicate that archaeal flagella are distinct from bacterial flagella in both their composition and probably also their assembly .…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of insertional mutations in flagellin genes in the archaeon Methanococcus voltae

Jarrell

Bayley

Florian

et al. 1996

Molecular Microbiology

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Methanococcus voltae is a flagellated member of the Domain Archaea that has four flagellin genes arranged in two transcriptional units. One transcriptional unit encodes only flaA while the second is a multi-cistronic unit encoding three flagellin genes (flaB1, flaB2, and flaB3) as well as at least seven other open reading frames downstream. The polymerase chain reaction was used to amplify an internal fragment of the flaA gene which was subsequently cloned into an insertion vector developed for M. voltae. Transformation of protoplasts with this vector led to the isolation of mutant strains that had insertions in flaA or flaB2. Mutant strains carrying insertions in flaA had flagelia that were similar to wild-type cells in both number and appearance when viewed using the electron microscope. In addition, some of these mutant strains had profiles identical to the wild type in immunoblots developed with antisera raised against the 31 kDa flagellin of M. voltae. All flaA mutant strains and the wild-type cells showed immuno-cross-reactive bands at 33 and 31 kDa (corresponding to purified flagellins) as well as at 18 kDa. Some flaA mutant strains also showed an immuno-cross-reactive band at 27 kDa which probably represents a truncated flagellin produced by the insertion vector. However, both types of flaA mutant strains were less motile than the wild type in semi-swarm plate experiments. The mutant strain with an insertion in flaB2 was non-flagellated when examined by electron microscopy and it was non-motile in semi-swarm plate experiments. It represents the first structural mutant strain isolated in a methanogen. This mutant strain lacked the 33, 31, and 18 kDa immuno-cross-reactive bands observed in the wild type and flaA mutant strains, and instead had a novel band at 20 kDa. This band may represent an unmodified flagellin which still has an attached leader peptide. If so, then one of the downstream genes in the multi-cistronic transcriptional unit may encode a leader peptidase for the flagellin system.

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Protein composition of Methanococcus thermolithotrophicus flagella

Cited by 12 publications

References 10 publications

Characterization of Flagellum Gene Families of Methanogenic Archaea and Localization of Novel Flagellum Accessory Proteins

Characterization of Flagellum Gene Families of Methanogenic Archaea and Localization of Novel Flagellum Accessory Proteins

The archaeal flagellum: a different kind of prokaryotic motility structure

Isolation and characterization of insertional mutations in flagellin genes in the archaeon Methanococcus voltae

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