Protein composition and native state of pigments of thylakoid membrane of wheat genotypes differently tolerant to water stress

Abstract

Protein composition and native state of chlorophylls were analyzed in two wheat (Triticum durum L.) genotypes with different tolerance to drought, Barakatli-95 (drought-tolerant) and Garagylchyg-2 (drought-sensitive), during water deficit. It is shown that the plants subjected to water deficit appear to have a slight increase in alpha- and beta-subunits of CF1 ATP-synthase complex (57.5 and 55 kD, respectively) in Barakatli-95 and their lower content in Garagylchyg-2. Steady-state levels of the core antenna of PS II (CP47 and CP43) and light-harvesting Chl a/b-apoproteins (LHC) II in the 29.5-24 kD region remained more or less unchanged in both wheat genotypes. The synthesis of 36 kD protein and content of low-molecular-weight polypeptides (21.5, 16.5, and 14 kD) were noticeably increased in the tolerant genotype Barakatli-95. Drought caused significant changes in the carotenoid region of the spectrum (400-500 nm) in drought-sensitive genotype Garagylchyg-2 (especially in the content of pigments of the violaxanthin cycle). A shift of the main band from 740-742 to 738 nm is observed in the fluorescence spectra (77 K) of chloroplasts from both genotypes under water deficiency, and there is a stimulation of the ratio of fluorescence band intensity F687/F740.

Drought Tolerance in Cotton: Involvement of Non‐enzymatic ROS‐Scavenging Compounds

“…Similarly, higher level of carotenoids and lower membrane damage characterizes the drought-exposed tolerant wheat genotype relative to the sensitive one (Chandrasekar et al 2000, Sairam andSaxena 2000). Recent data describing the modified absorption spectra of carotenoids in drought-sensitive wheat genotypes in contrast to the conserved spectra of tolerant ones (Guseynova et al 2006) give support to our result.…”

“…2000, Sairam and Saxena 2000). Recent data describing the modified absorption spectra of carotenoids in drought‐sensitive wheat genotypes in contrast to the conserved spectra of tolerant ones (Guseynova et al. 2006) give support to our result.…”

A Proteomic Profiling Approach to Reveal a Novel Role of Brassica napus Drought 22 kD/Water-Soluble Chlorophyll-Binding Protein in Young Leaves during Nitrogen Remobilization Induced by Stressful Conditions

“…In a drought-resistant genotype of wheat (Triticum aestivum), the induction of a protein homologous to BnD22 identified in the thylakoid membrane showed a significant role for BnD22 during the stress response (Guseynova et al, 2006). In our study, BnD22 represented up to 9% of the leaf soluble protein content in plants treated with MeJA, whereas it represented approximately 1% of the soluble protein content in plants adapted to drought (Downing et al, 1992;Ilami et al, 1997).…”

Effect of Drought on the Gas Exchange, Chlorophyll Fluorescence and Yield of Six Different‐Era Spring Wheat Cultivars

“…The decrease in the content of D1 and D2 proteins in the thylakoid membranes from water‐stressed plants (especially in the modern cultivars Lc8275 and Gc20) seems to be due, in part, to their enhanced stress tolerance (Guseynova et al. ). High rates of D1‐protein turnover provide stability to thylakoid membranes and their electron transport chain from the damaging action of free radicals formed under stress conditions (Fan et al.…”

“…Lu and Zhang (1999) and Shangguan et al (2000) also suggested that under mild water stress, PS2 photochemistry was not affected and was resistant to water deficits, supporting the observation that gs was important in reducing P N . The decrease in the content of D1 and D2 proteins in the thylakoid membranes from water-stressed plants (especially in the modern cultivars Lc8275 and Gc20) seems to be due, in part, to their enhanced stress tolerance (Guseynova et al 2006). High rates of D1-protein turnover provide stability to thylakoid membranes and their electron transport chain from the damaging action of free radicals formed under stress conditions (Fan et al 2009).…”