Protein Characterization of Extracted Water from Three Kinds of Edible Bird Nest Using SDS-PAGE CBB Staining and SDSPAGE Glycoprotein Staining and LC-MS/MS Analyses

Utomo, Budi; Rosyidi, Djalal; Radiati, LE; Puspaningsih, NNT; Proborini, WD

doi:10.9790/2380-07933338

Cited by 9 publications

(5 citation statements)

References 7 publications

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“…This is also due to the 7.0 KDa cut-off molecular weight of the dialysis tubing used to separate the mixture. The protein size of EBN extract was 140.8, 64.8, and 21.1 kDa, whereas the molecular weight of EBN glycoprotein was 140.8 and 64.8 kDa [39]. Raw EBNs depict more and distinct bands as compared to processed EBNs, where the EBN processing may have reduced the original amount of intact protein, thus resulting in different protein profiles [4].…”

Section: Molecular Weight Distribution Of Protein In Extracts and Hydrolysates Of Ebnsmentioning

confidence: 99%

Characterization and Extraction Influence Protein Profiling of Edible Bird’s Nest

Nasir

Ibrahim

Bakar

et al. 2021

Foods

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The edible bird nest (EBN) from Aerodramus fuciphagus has been consumed as a Chinese traditional food for health and medicinal purposes due to its elevated nutritional value. The present study focused on the influence of characterization and extraction methods on protein profiling, which could be a guideline for grading the EBN. The proposed extraction method is similar to the common food preparation methods of consumers and thus can accurately establish the bioactive protein available upon human consumption. The characterization includes physicochemical analysis (physical, morphology, elemental composition, and microbial content) and chemical analysis (crude protein and amino acid). The morphology of half-cup EBN was found to be uniformly shaped and rich in calcium as compared to rough surface of stripe-shaped EBN, and there was no significant microbial growth in both types of EBN. The crude protein and amino acid content in half-cup EBN were significantly higher than stripe-shaped EBN. The full stew (FS) and stew (SE) extraction methods produced a maximal yield of soluble protein. Sialic acid content in SE extract (8.47%, w/w) and FS extract (7.91%, w/w) were recorded. About seven parent proteins (39.15 to 181.68 kDa) were identified by LC-MS/MS Q-TOF, namely 78 kDa glucose-regulated protein, lysyl oxidase-3, Mucin-5AC-like, acidic mammalian chitinase-like, 45 kDa calcium-binding protein, nucleobindin-2, and ovoinhibitor-like. In conclusion, the characteristics and extraction methods influence the availability of bioactive protein and peptides, demonstrating the potential usage of EBN in improving its biological activities and nutritional properties.

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Section: Molecular Weight Distribution Of Protein In Extracts and Hydrolysates Of Ebnsmentioning

confidence: 99%

Characterization and Extraction Influence Protein Profiling of Edible Bird’s Nest

Nasir

Ibrahim

Bakar

et al. 2021

Foods

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“…Hasil yang diperoleh dari protein SBW setelah dicerna hampir mirip dengan protein serum sapi yang mencapai tingkat kecernaan 95% (Deutz et al, 1995). Berat molekul protein SBW bervariasi, sekitar 140,8, 64,8 dan 21,2 kDa (Utomo et al 2014). Sebagian besar protein berukuran besar dicerna menjadi 70 kDa, 40 kDa dan di bawahnya, sementara beberapa protein besar lainnya tetap stabil (Xian et al, 2010).…”

Section: Sifat Fisikokimia Bahan Bioaktif Sarang Burung Waletunclassified

Ulasan Sarang Burung Walet Sebagai Pangan Fungsional

Wahyuni

2021

Acta Vet Indones

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Sarang burung walet (SBW) merupakan sarang yang berasal dari saliva beberapa spesies burung walet keluarga Apodidae, terutama spesies Aerodramus fuciphagus dan A. maximus. Indonesia merupakan negara penghasil SBW terbesar dengan menyumbang 85% dari pasar dunia. Sarang burung walet sejak lama telah digunakan dalam pengobatan tradisional Tiongkok. Komponen utama dari SBW adalah glikoprotein, yang diyakini terlibat dalam jalur biologis yang beragam untuk meningkatkan kesehatan. Berbagai metode penelitian telah dilakukan untuk mengkarakterisasi peptida dan glikoprotein SBW sebagai bahan bioaktif makanan fungsional. Ulasan ini bertujuan untuk memberikan informasi mengenai potensi SBW sebagai produk makanan fungsional berdasarkan kandungan bahan bioaktif dan sifat fisikokimia yang dimilikinya. Sarang burung walet digunakan sebagai bahan makanan, baik dengan menyajikan dalam bentuk utama atau menggabungkan dengan bahan lain yang meningkatkan nilai tambah produk pangan seperti minuman siap saji, yoghurt, roti daging, es krim, mie dan cokelat. Potensi komponen bioaktif SBW sebagai pangan fungsional meliputi klaim penurunan risiko penyakit dan klaim fungsi lain. Klaim penurunan risiko penyakit antara lain anti inflamasi, aktifitas antivirus, meningkatkan kesehatan jantung dan pembuluh darah, meningkatkan imunitas, efek neuroprotektif, dan mencegah penyakit diabetes. Klaim fungsi lain meliputi ploriferasi sel, aktifitas epidermal growth factor (EGF) dan anti penuaan, memperbaiki fungsi saluran pencernaan, peningkatan kekuatan tulang, meningkatkan fungsi saluran reproduksi, dan antioksidan.

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“…No. Monosaccharide Kathan & Weeks [33] Tung et al [34] Chua et al [35] [41] Purification of house EBN's sialyl-glycoprotein showed the abundance molecular weight of 128 kDa (19.1%), 106 kDa (18.5%) and 43 kDa (29.4%). The sialyl-glycoprotein with 43 kDa contained terminal α2-3 and α2-6 sialic acid linkage.…”

Section: Nomentioning

confidence: 99%

Edible Bird’s Nest: Physicochemical Properties, Production, and Application of Bioactive Extracts and Glycopeptides

Daud

Yusop

Babji

et al. 2019

Food Reviews International

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Edible bird's nest (EBN), the swiftlet's nest, has been consumed for centuries as a tonic or health food. The information upon the usage and benefits of the EBN are largely based on historical and observational reports since its discovery, until some were proven scientifically at recent times. Studies have been conducted on the major components of the EBN, i.e. glycoprotein, in attempts to elucidate the roles of the glycoprotein in terms of functionality toward health improvement. This article presents a review of the physicochemical and nutritional composition of EBN from different harvested locations and its functional properties. The conversion of EBN glycoproteins to glycopeptides via suitable hydrolysis methods to produce bioactive EBN glycopeptides was demonstrated, and it showed improvements in the functionality and nutritional values of EBN. Some applications of EBN glycopeptides into the new development of food and beverage products showed positive results, implicated to the bioavailability of the EBN glycopeptides. The combined information in this review will benefit the scientific communities, EBN manufacturers, and nutraceutical industries.

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Protein Characterization of Extracted Water from Three Kinds of Edible Bird Nest Using SDS-PAGE CBB Staining and SDSPAGE Glycoprotein Staining and LC-MS/MS Analyses

Abstract: Abstract:The objective of this research is to see the comparison of protein characterization from three kinds of

Cited by 9 publications

References 7 publications

Characterization and Extraction Influence Protein Profiling of Edible Bird’s Nest

Characterization and Extraction Influence Protein Profiling of Edible Bird’s Nest

Ulasan Sarang Burung Walet Sebagai Pangan Fungsional

Edible Bird’s Nest: Physicochemical Properties, Production, and Application of Bioactive Extracts and Glycopeptides

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