Protein Changes Associated with Adventitious Root Formation in Hypocotyls of Pinus Radiata

Li, Mingshan; Leung, David W. M.

doi:10.1023/a:1017910018880

Cited by 8 publications

(2 citation statements)

References 20 publications

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“…However, these auxins intervent in the synthesis of proteins already present in the plant. Other authors [31] showed that enzyme content begins to increase in IBA treatment in Pinus radiata during the first day but intensifies at 7 days. These results are similar to the results obtained during rhizogenesis in Gnetum (spp.).…”

Section: Discussionmentioning

confidence: 91%

Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in Gnetum spp.

Minyaka¹,

Claude²,

Apollin³

et al. 2019

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Root failure is a key limiting factor in Gnetum spp. domestication and production of plants materials for farming. To overcome, root failure in Gnetum spp. may depend on adequate strategies set up and understanding of biochemical markers controlling the rooting process. The present work is principally based on phenolic compounds contents, peroxidasic and polyphenol oxidasic activities during rooting of cuttings (pretreated with IBA, ANA and IAA) at induction, initiation and expression phases of roots formation in Gnetum africanum Welw. and G. buchholzianum Engl. The study revealed that cuttings pretreated with IBA (150 mg/l) were more effective than IAA and NAA. Phenolic compounds analysis revealed that cuttings pretreated with IBA exhibited high phenolic compound contents at initiation and expression phases for both Gnetum species. Indicating that IBA pretreatment promotes phenolic compounds synthesis and accumulation necessary for roots differentiation. Enzyme contents appear to be high during the initiation stage for both species when they are pretreated with IBA (150 mg/l). Peroxidasic and Polyphenol oxidasic activities evolved on the synchroneous maner. This allows us to know that the implication of these enzymes in the rooting process is fundamental makers.

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Section: Discussionmentioning

confidence: 91%

Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in Gnetum spp.

Minyaka¹,

Claude²,

Apollin³

et al. 2019

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“…In relation to this, Sorin et al [24] reported that in Arabidopsis superroot mutants (sur1 and sur2), which are auxin over-producers and spontaneously generate adventitious roots, the auxin induces GH3-like protein is positively correlated with adventitious root number. In P. radiata, there is only one report indicating the differential accumulation of 16 proteins through hypocotyls adventitious rooting [25]. However, these peptides were not identified.…”

Section: Introductionmentioning

confidence: 97%

Proteomic Analysis through Adventitious Rooting of Pinus radiata Stem Cuttings with Different Rooting Capabilities

Álvarez¹,

Valledor²,

Sáez³

et al. 2016

AJPS

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In forest production systems, vegetative propagation of elite clones through adventitious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts have been made to identify markers or/and regulators of the aging process and adventitious rooting, molecular mechanisms that regulate both processes are scarcely known, especially at protein level. This research evaluated qualitative and quantitative changes in protein accumulation during the adventitious rooting process of P. radiata stem cuttings, with different rooting capabilities. Beside, an analysis of morpho-anatomical changes was performed in stem cuttings with high and low rooting capabilities, during the adventitious rooting process. It was observed that juvenile 1-year-old stem cuttings rooted in a 100%, while aged stem cuttings (3-year-old) presented only a 20% of rooting. According to the results of differential protein accumulation, univariate and multivariate analysis indicated that in total, 114 and 89 proteins were differentially accumulated in juvenile and aged cuttings, respectively. Also, identification of such proteins showed the presence of proteins related to cell wall organization and the presence of a protein related with proper distribution of auxin PIN transporter, both key in the new meristem formation process during adventitious rooting.

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Adventitious Root Formation: New Insights and Perspectives

Geiss

Gutierrez

Bellini

2018

Annual Plant Reviews Online

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The root system of a plant consists of the primary, lateral and adventitious roots. Lateral roots always develop from roots whereas adventitious roots form from stem or leaf‐derived cells. Adventitious rooting is an essential step in the vegetative propagation of economically important horticultural and woody species. It allows clonal propagation and rapid fixation of superior genotypes prior to their introduction into production or breeding programs. Problems associated with rooting of cuttings frequently result in significant economic losses. Development of adventitious roots is a complex process that is affected by multiple factors including phytohormones, light, nutritional status, associated stress responses such as wounding, and genetic characteristics. How endogenous and environmental factors interact to control adventitious root formation is still poorly understood, although significant progress has been made in the understanding of the molecular control of root and lateral root development. In this review, we will summarize the current knowledge on the physiological aspects of AR formation and highlight the recent progress made in the identification of putative molecular players involved in the control of adventitious rooting.

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Protein Changes Associated with Adventitious Root Formation in Hypocotyls of Pinus Radiata

Cited by 8 publications

References 20 publications

Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in <i>Gnetum</i> spp.

Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in <i>Gnetum</i> spp.

Proteomic Analysis through Adventitious Rooting of <i>Pinus radiata</i> Stem Cuttings with Different Rooting Capabilities

Adventitious Root Formation: New Insights and Perspectives

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Protein Changes Associated with Adventitious Root Formation in Hypocotyls of Pinus Radiata

Cited by 8 publications

References 20 publications

Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in &lt;i&gt;Gnetum&lt;/i&gt; spp.

Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in &lt;i&gt;Gnetum&lt;/i&gt; spp.

Proteomic Analysis through Adventitious Rooting of &lt;i&gt;Pinus radiata&lt;/i&gt; Stem Cuttings with Different Rooting Capabilities

Adventitious Root Formation: New Insights and Perspectives

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Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in <i>Gnetum</i> spp.

Influence of Exogenous Auxins on Phenolic Compounds Contents and Polyphenol oxidasic and Peroxidasic Activities in Root Differentiation in <i>Gnetum</i> spp.

Proteomic Analysis through Adventitious Rooting of <i>Pinus radiata</i> Stem Cuttings with Different Rooting Capabilities