Protein c-phycocyanin, structure, physicochemical and biological properties, methods of extraction

Hudz, Nataliia; Turkina, V. A.; Filipska, Anna; Kuzminov, O.; Korytniuk, Raisa; Lubenets, V. І.; Wieczorek, Piotr; Savickienė, Nijolė

doi:10.7124/bc.000a66

Cited by 1 publication

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References 28 publications

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“…[3][4][5][6][7][8][9]. C-PC stimulates the immune system and exhibits hepatoprotective, antiplatelet and neuroprotective activities as well [10,11]. Many authors state about the significant antioxidant activity of C-PC [9,12,13].…”

Section: R E C O O P H S T a S S O C I A T I O N R E C O O P H S T A ...mentioning

confidence: 99%

Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)

Hudz¹,

Turkina²,

Yezerska³

et al. 2022

Ukr.Biochem.J.

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The physicochemical characteristics of phycocyanin extracted from cyanobacteria collected in Kaunas Lagoon were studied (spectrum characteristics, C-PC content in the dry mass and chemical purity). It was determined that the tested concentrations of C-PC in purified water should be in the range of 0.02–0.16% for measuring C-PC content in the dry mass and its spectrum characteristics. The two clear absorption maxima were detected in the spectrum of C-PC at the wavelengths of 277 and 619 nm. The content of C-PC in the dry powder form was in the range of 7.25% to 9.30% depending on its concentration in the solution and type of spectrophotometer. Furthermore, a purity factor of 1.5 was calculated, which indicated the food qualification of the obtained biomass of C-PC. Finally, the analytical procedure for studying the pro- and anti-oxidant activity of C-PC was developed and the antioxidant activity of C-PC was measured for the available markers. It was revealed that C-PC has dual properties (pro- and anti-oxidant ones) depending on its concentration, more exactly, its content in reaction mixtures with 2,2-diphenyl-1-picrylhydrazyl (DPPH). The following issues were resolved during the research: the concentration of ethanol in the DPPH solution was chosen in order to avoid precipitation of proteins in the reaction mixtures (50%); the ratio of the solution of C-PC to the DPPH solution was selected; the selected concentrations of the markers for the construction of their calibration curves were chosen for quercetin and for rutin. The antioxidant activity of the obtained C-PC sample was determined. Keywords: antioxidant activity, C-phycocyanin, cyanobacteria, DPPH, quercetin, rutin

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Section: R E C O O P H S T a S S O C I A T I O N R E C O O P H S T A ...mentioning

confidence: 99%

Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)

Hudz¹,

Turkina²,

Yezerska³

et al. 2022

Ukr.Biochem.J.

View full text Add to dashboard Cite

show abstract

Protein c-phycocyanin, structure, physicochemical and biological properties, methods of extraction

Cited by 1 publication

References 28 publications

Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)

Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)

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