Protein biosynthesis in aging mouse tissues

Du, Julie T.; Beyer, Thomas A.; Lang, Calvin A.

doi:10.1016/0531-5565(77)90004-3

Cited by 36 publications

(5 citation statements)

References 21 publications

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“…In parotid glands of the rat, the rate of protein synthesis declines progressively between the ages of 2 and 30 months, as indicated by the reduction in the rate of incorporation of labelled amino acids into the acid-insoluble proteins of this gland [6,7]. These findings are consistent with the results of many previous studies, which have reported the age-related decline in protein synthesis in different types of organs and tissues, such as brain [9][10][11][12][13], muscle [14][15][16], testis [17], liver [18][19][20][21][22][23][24][25][26][27][28][29] and heart [28].…”

Section: Introductionsupporting

confidence: 89%

“…However, it does not appear that all cellular proteins are uniformly affected during aging. As demonstrated in the livers of rats [28,30,31] and mice [28], the synthesis of albumin increases with age, and the synthesis of ferritin does not change [30], while that of heme protein declines [28]. These results suggest that the synthesis of proteins with different functions is affected during aging, depending on the cellular needs for these proteins, and that the age-related changes in some proteins can be masked if only the synthesis of total proteins is investigated [30].…”

Section: Introductionmentioning

confidence: 77%

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Changes in the synthesis of exportable and nonexportable proteins in parotid glands during aging

Kim

Calkins

Weinhold

et al. 1982

Mechanisms of Ageing and Development

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The age-related differences in the synthesis of exportable and nonexportable proteins of the parotid salivary gland were compared in 2- and 24-months-old rats. Parotid slices from these rats were incubated in the presence of [14C]leucine and the amount of radioactivity incorporated into the water-soluble proteins of the postmicrosomal supernatant was compared. The exportable and nonexportable proteins were identified by electrophoretic separation of these proteins by comparing the banding patterns of the gel preparations from unstimulated glands to those from the glands stimulated to secrete. The radioactivity determination in various protein bands from these rats indicated that the synthesis of exportable secretory proteins declined with age, while that of nonexportable proteins did not appear to change.

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Section: Introductionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 77%

Changes in the synthesis of exportable and nonexportable proteins in parotid glands during aging

Kim

Calkins

Weinhold

et al. 1982

Mechanisms of Ageing and Development

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“…Previous studies have established that the total endogenous leucine con tent of liver tissue of either mice [2] or -rats [5] does not vary with age anc preliminary experiments indicate that this holds as well for isolated rat hepatocytes [Note 11. Inasmuch as rat hepatocytes have also been reported freely permeable to leucine [4,9], we have consistently utilized leucine levels in incubations which exceed the average endogenous hepatocyte content by a minimum factor of twenty-fold.…”

Section: Viskup/baker/holbrook/penni Allmentioning

confidence: 81%

Age-associated changes in activities of rat hepatocytes. I. Protein synthesis

Viskup

Baker

Holbrook

et al. 1979

Experimental Aging Research

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Experiments involving simultaneous comparisons of cells from two animals establish an age-dependent decline in protein synthesis. Specific radioactivities of fractions from cells of aged animals (20+ months) are depressed 60% to 80% versus those from younger animals (2 to 14 months). Isotope ratio patterns derived by PAGE indicate that formation of a 20000 MW polypeptide of cytoribosomal origin is even more strongly and specifically depressed.

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“…This was based on findings of lower levels of NADP+-linked enzyme activities (1,2), NADPH/NADP+ ratios (3), protein biosynthesis, and DNA replication in vivo (4)(5)(6). This was based on findings of lower levels of NADP+-linked enzyme activities (1,2), NADPH/NADP+ ratios (3), protein biosynthesis, and DNA replication in vivo (4)(5)(6).…”

mentioning

confidence: 99%

Dietary Nordihydroguaiaretic Acid Increases the Life Span of the Mosquito

Richie

Mills

Lang

1986

Experimental Biology and Medicine

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Our previous findings indicated that a major characteristic of aging organisms is a decrease in reducing capacity. Our objectives were to correct this impairment by administration of nordihydroguaiaretic acid (NDGA), a potent reducing agent, and to determine the effect on adult longevity of the mosquito. NDGA supplements were included in the axenic larval medium or adult diet of mosquitoes of different ages. The mean adult life spans of both sexes increased 42-64% over controls (P < 0.025), and the most effective doses were 0.001% for females and 0.005% for males. This NDGA effect was dependent on the age when feeding was initiated, since only biosynthetically active larvae and young adults were responsive. Also the effect was not due to dietary restriction. These results confirm the life span-enhancement effect of NDGA using defined conditions and establish the importance of redox status in the aging process. o

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Protein biosynthesis in aging mouse tissues

Cited by 36 publications

References 21 publications

Changes in the synthesis of exportable and nonexportable proteins in parotid glands during aging

Changes in the synthesis of exportable and nonexportable proteins in parotid glands during aging

Age-associated changes in activities of rat hepatocytes. I. Protein synthesis

Dietary Nordihydroguaiaretic Acid Increases the Life Span of the Mosquito

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