Protein biomarkers for early detection of diseases: The decisive contribution of combinatorial peptide ligand libraries

Boschetti, Egisto; D’Amato, Alfonsina; Candiano, Giovanni; Righetti, Pier Giorgio

doi:10.1016/j.jprot.2017.08.009

Cited by 45 publications

(25 citation statements)

References 136 publications

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“…Proteomics, i.e., the large-scale study of proteins, is emerging as a powerful technology to assist in the identification of suitable biomarkers of clinical relevance in PC [ 7 ]. Historically, the most widely adopted proteomic technique has been two-dimensional electrophoresis (2DE), which offers high resolution for separating proteins within complex protein mixtures [ 8 ]. The two-dimensional difference gel electrophoresis (2D-DIGE) variant allows direct comparison of two protein samples on the same gel, combining conventional 2DE with the sensitivity of fluorescent labeling [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomic biomarkers in body fluids associated with pancreatic cancer

et al. 2018

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Pancreatic cancer (PC) is a highly malignant disease that represents the fourth leading cancer-related death worldwide. There has been very little improvement in survival rates over recent years, and surgical resection remains the only reliable curative approach. Factors that contribute to this dismal prognosis for PC include its rapid progression and invasion, the absence of specific symptoms, and the little impact of available chemotherapy. Importantly, the management of this malignancy is also limited by the lack of highly specific and sensitive biomarkers for its diagnosis and follow-up, and their identification is therefore considered a promising strategy to improve outcomes in these patients. Numerous translational studies have explored the usefulness of body fluids as a non-invasive source of PC-specific biomarkers, and innovations in proteomic methods and technologies have provided a myriad of protein biomarkers for different cancers. The adoption of a proteomic approach has improved understanding of the biology of PC and contributed to the potential identification of protein biomarkers for this disease. This review considers the most recent research efforts to develop novel proteomic biomarkers in body fluids for PC.

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Section: Introductionmentioning

confidence: 99%

Proteomic biomarkers in body fluids associated with pancreatic cancer

et al. 2018

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“…The levels of such potential biomarkers are too low to allow for any reasonable identification. They become visible only at the last stage of cancer invasion, unless one tries to concentrate them by the combinatorial peptide ligand library technique . Also in Chekhov's case, it was also quite unique to detect abundant traces of Koch bacillus in the collar of his shirt.…”

Section: Discussionmentioning

confidence: 99%

Il n'y a pas d'amour heureux pour Casanova: Chemical‐ and bio‐analysis of his Memoirs

Zilberstein

et al. 2019

Electrophoresis

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The original manuscript of Casanova's Memoirs is stored at the Bibliothèque Nationale de France in Paris. We have gained access to it and explored the surfaces of chapters one and two (via the ethylene vinyl acetate [EVA] film technology, i.e., of diskettes of ethylene vinyl acetate with embedded strong cation and anion exchangers and C8 resins) in search of potential diseases of the author, especially of the gonorrhea bacterium, since Casanova reported that he had several bouts of this pathology along his adventurous life. Although the bacterium was not found, we have detected high levels of HgS as red spots along the lines of the manuscript, suggesting that Casanova was using this chemical as a cure for his venereal disease. Additionally, among the several bacteria identified on the surface via mass spectrometry, we could detect traces of Streptococcus uberis, a typical animal infection, found also in humans, together with a few strains of Lactobacilli, probably present in his saliva. The EVA film technology appears to open new horizons for investigating the world Cultural Heritage.

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“…35,44,45 Aspects of quality of life must be taken into consideration. On another side of the RCC disease spectrum, searches for early detection biomarkers should be prioritized 35,45,46 to aid timely actions against the “emperor of all maladies” 47 for greater curability.…”

Section: Discussionmentioning

confidence: 99%

Cytokine Measurements for Diagnosing and Characterizing Leukemoid Reactions and Immunohistochemical Validation of a Granulocyte Colony-Stimulating Factor and CXCL8-Producing Renal Cell Carcinoma

et al. 2018

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Background:Various paraneoplastic syndromes are encountered in renal cell carcinomas. This case report illustrates that a paraneoplastic leukemoid reaction may precede the diagnosis of renal cell carcinoma and be explained by cytokine production from the cancer cells.Case presentations:A 64-year-old man was referred for hematology workup due to pronounced leukocytosis. While being evaluated for a possible hematologic malignancy as the cause, he was found to have a metastasized renal cell carcinoma, and hyperleukocytosis was classified as a leukemoid reaction. A multiplex panel for measurement of 25 serum cytokines/chemokines showed highly elevated levels of granulocyte colony-stimulating factor (G-CSF) and CXCL8 (C-X-C-motif chemokine ligand 8, previously known as interleukin [IL]-8). By immunohistochemistry it was shown that the renal carcinoma cells expressed both these cytokines. Two additional, consecutive patients with renal cell carcinoma with paraneoplastic leukocytosis also showed elevated serum levels of CXCL8, but not of G-CSF. Nonparametric statistical evaluation showed significantly higher serum concentrations of CXCL8, IL-6, IL-10, monocyte chemoattractant protein 1 (MCP-1), and tumor necrosis factor, but lower interferon gamma (IFN-γ) and IL-1α, for the 3 renal cell carcinoma cases compared with healthy blood donors.Conclusions:In suspected paraneoplastic leukocytosis, multiplex serum cytokine analyses may facilitate diagnosis and provide an understanding of the mechanisms for the reaction. In the index patient, combined G-CSF and CXCL8 protein expression by renal carcinoma cells was uniquely documented. A rapidly fatal course was detected in all 3 cases, congruent with the concept that autocrine/paracrine growth signaling in renal carcinoma cells may induce an aggressive tumor phenotype. Immune profiling studies could improve our understanding for possible targets when choosing therapies for patients with metastatic renal cell carcinoma.

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Protein biomarkers for early detection of diseases: The decisive contribution of combinatorial peptide ligand libraries

Cited by 45 publications

References 136 publications

Proteomic biomarkers in body fluids associated with pancreatic cancer

Proteomic biomarkers in body fluids associated with pancreatic cancer

Il n'y a pas d'amour heureux pour Casanova: Chemical‐ and bio‐analysis of his Memoirs

Cytokine Measurements for Diagnosing and Characterizing Leukemoid Reactions and Immunohistochemical Validation of a Granulocyte Colony-Stimulating Factor and CXCL8-Producing Renal Cell Carcinoma

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