Protein binding to supported lipid membranes: investigation of the cholera toxin-ganglioside interaction by simultaneous impedance spectroscopy and surface plasmon resonance

Terrettaz, Samuel; Stora, T.; Duschl, Claus; Vogel, Horst

doi:10.1021/la00029a033

Cited by 244 publications

(228 citation statements)

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“…Therefore, we conclude that, on alkylthiol templates on gold, a perfectly closed outer lipid layer can be formed that blocks completely unspecific protein binding. A similar effect was observed for cholera toxin and phosphatidylcholine layers (Terrettaz et al, 1993). Nonspecific antibody binding to supported lipid bilayers on waveguides must at least partly be attributed to the particular thiolipid used.…”

Section: Protein Binding To Functionalized Membranessupporting

confidence: 66%

“…This property of lipid membrane surfaces has been adopted for the development of biocompatible materials (Chapman, 1993) and might also be useful for sensor surfaces (Lang et al, 1993(Lang et al, , 1994Terrettaz et al, 1993). The remaining nonspecific binding of protein to supported membranes on sensor surfaces might be further reduced using thiolipids with long hydrophilic spacer groups for the template layer or using lipids presenting flexible chains of oligoethoxides or sugar moieties at the outer membrane surface.…”

Section: Discussionmentioning

confidence: 99%

“…After thiolipid binding to the waveguides, lipid bilayers of POPC, of POPC/biotin-DPPE, or of POPC/lipopeptide in selected molar ratios were formed by vesicle spreading (Kalb et al, 1992;Lang et al, 1992Lang et al, , 1994Terrettaz et al, 1993) or by dilution of mixed micelle solutions (Lang et al, 1994). Vesicle dispersions were produced by first drying down a chloroform solution of 1 mg either of POPC or a mixture of POPC and biotin-DPPE.…”

Section: Formation Of the Lipid Bilayer And Protein Bindingmentioning

confidence: 99%

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Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

et al. 1995

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A new method is presented for measuring sensitively the interactions between ligands and their membrane-bound receptors in situ using integrated optics, thus avoiding the need for additional labels. Phospholipid bilayers were attached covalently to waveguides by a novel protocol, which can in principle be used with any glass-like surface.In a first step, phospholipids carrying head-group thiols were covalently immobilized onto Si02-Ti02 waveguide surfaces. This was accomplished by acylation of aminated waveguides with the heterobifunctional crosslinker N-succinimidyl-3-maleimidopropionate, followed by the formation of thioethers between the surface-grafted maleimides and the synthetic thiolipids. The surface-attached thiolipids served as hydrophobic templates and anchors for the deposition of a complete lipid bilayer either by fusion of lipid vesicles or by lipid self-assembly from mixed lipid/detergent micelles. The step-by-step lipid bilayer formation on the waveguide surface was monitored in situ by an integrated optics technique, allowing the simultaneous determination of optical thickness and one of the two refractive indices of the adsorbed organic layers. Surface coverages of 50-60% were calculated for thiolipid layers. Subsequent deposition of POPC resulted in an overall lipid layer thickness of 45-50 A , which corresponds to the thickness of a fluid bilayer membrane.Specific recognition reactions occurring at cell membrane surfaces were modeled by the incorporation of lipidanchored receptor molecules into the supported bilayer membranes. (1) The outer POPC layer was doped with biotinylated phosphatidylethanolamine. Subsequent specific binding of streptavidin was optically monitored. (2) A lipopeptide was incorporated in the outer POPC monolayer. Membrane binding of monoclonal antibodies, which were directed against the peptide moiety of the lipopeptide, was optically detected. The specific antibody binding correlated well with the lipopeptide concentration in the outer monolayer.

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Section: Protein Binding To Functionalized Membranessupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Formation Of the Lipid Bilayer And Protein Bindingmentioning

confidence: 99%

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Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

et al. 1995

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“…9 The most common approach employed for the surface immobilisation of phospholipids entails the formation of a surface-supported lipid bilayer. 10 These are typically prepared using surface-bound alkylsilane, 11,12 alkanethiol, 7,13,14,15 or thiolipid 10,16 self-assembled monolayers (SAMs). The inherent well-packed nature of such SAMs enables the formation of a non-interdigitated hybrid lipid bilayer comprising a layer of phospholipids on top of a layer of surface bound SAM alkyl chain molecules.…”

Section: Introductionmentioning

confidence: 99%

Combining plasmachemical emulsion-templating with ATRP to create macroporous lipophilic surfaces

Brown

Wood

Badyal

2014

Journal of Colloid and Interface Science

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Publisher's copyright statement: NOTICE: this is the author's version of a work that was accepted for publication in Journal of Colloid and Interface Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be re ected in this document. Changes may have been made to this work since it was submitted for publication. A de nitive version was subsequently published in Journal of Colloid and Interface Science, 421, 2014, 10.1016/j.jcis.2014.01.019. Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. ABSTRACTWell-defined alkyl chain side group polymer brushes have been tethered onto high surface area macroporous pulsed plasmachemical emulsion-templated poly(vinylbenzyl chloride) initiator layers (typically 600-700 m 2 g -1 ) using atom transfer radical polymerisation (ATRP). Immobilisation of phospholipids onto these bioactive surfaces is found to occur through interdigitation, and the efficacy of lipid binding is governed by the alkyl side group chain length of the polymer brushes.

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“…Atualmente encontra-se aplicação em uma faixa extensa de materiais, para determinação de características de superfícies, incluindo propriedades dielétricas, processos de adsorção, degradação ou hidratação de superfícies. Estes estudos tornaram-se realidade a partir da deposição de filmes de Langmuir-Blodgett ou monocamadas auto-organizadas (SAMs -"self-assembled monolayers") na superfície metálica 87,88 .…”

Section: Aplicações Físicasunclassified

SPR: Uma nova ferramenta para biossensores

2003

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Recebido em 4/1/02; aceito em 27/6/02 SPR -NEW TOOL FOR BIOSENSORS. This paper reviewed the development and theoretical aspects of surface plasmon ressonance (SPR) technique and discusses this powerful sensor technology in the development of biosensors, as well as for the investigation of biological interactions and clinical assays. The SPR has been proven to be a valuable tool to investigate dynamic processes, such as adsorption, degradation, determination of dieletric properties, association/dissociation kinetics, affinity constants of specific ligand-ligate interactions, allowing real-time analysis at almost any surface. The SPR as a complementary technique alongside electrochemical methods is also presented.

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Protein binding to supported lipid membranes: investigation of the cholera toxin-ganglioside interaction by simultaneous impedance spectroscopy and surface plasmon resonance

Cited by 244 publications

References 0 publications

Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

Combining plasmachemical emulsion-templating with ATRP to create macroporous lipophilic surfaces

SPR: Uma nova ferramenta para biossensores

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