Protein binding properties of surface-modified porous polyethylene membranes

Greene, George W.; Radhakrishna, Harish; Tannenbaum, Rina

doi:10.1016/j.biomaterials.2005.03.025

Cited by 29 publications

(17 citation statements)

References 58 publications

(101 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, these data presented previously inferred that Run 12 contained the highest functionalized group (carbonyl group) that play significant role in binding protein to polymer surface (Greene et al, 2005) and the most effective condition to prepare PS surface for binding gelatin compared to other conditions suggested by the experimental design. Therefore, slight changes on time will affect the protein binding properties of the PS particle.…”

Section: Discussionmentioning

confidence: 69%

Surface Modification of Polystyrene Beads by Ultraviolet/Ozone Treatment and its Effect on Gelatin Coating

Yusilawati¹

2010

American Journal of Applied Sciences

View full text Add to dashboard Cite

Problem statement: Polystyrene failed to provide any reactive functionality of surface hydrophilicity that is capable of binding proteins. It is known that polystyrene must be chemically modified to make its surface amenable to covalent crosslinking with protein. Approach: The aim of this study was to investigate the effects of UV/ozone treatment on gelatin coating. The surfaces of microsize polystyrene beads (150 Âµm) were modified by UV/ozone treatment system at different treatment time, ozone flow-rate and UV intensity was analyzed by Design expert software. The treated beads were characterized with ATR-FTIR analysis to determine the introduction of carbonyl (-C=O), carboxylic group (-COOH) and amide group (-CO-NH₂) onto the polystyrene surface. Sample characterization was also carried out by SEM and densitometer. Gelatin immobilization was then preceded by incubating treated PS sample in gelatin solution and the total amount of gelatin coated on the modified surface was identified by Bradford assay. Results: The maximum amount of gelatin obtained was 63.75 Âµg mL^-1 while the lowest amount obtained for untreated PS (9.947 Âµg mL^-1). The introduction of carbonyl, hydroxyl and amide group on the polystyrene beads surface was confirmed by ATR-FTIR analysis and thus measures the importance of UV/ozone treatment. Conclusion: From the results, it has been found that time is the most significant factor to prepare samples for gelatin immobilization at reduced flow rate and at an increased Ultraviolet (UV) intensity in the ranges of study

show abstract

Section: Discussionmentioning

confidence: 69%

Surface Modification of Polystyrene Beads by Ultraviolet/Ozone Treatment and its Effect on Gelatin Coating

Yusilawati¹

2010

American Journal of Applied Sciences

View full text Add to dashboard Cite

show abstract

“…During inflammation microcapsules may be exposed to variations in pH. 43 Factors such as solution electrolyte concentration and pH have a considerable impact on the strength and type of the surface charges, 46 in turn the surface charge property of a material will influence protein adsorption process. 39 Thus, solution electrolyte concentration and pH are often used to study electrostatic interaction between biomaterials and proteins.…”

Section: Effect Of Ph and Ionic Strength Dependencementioning

confidence: 99%

Effect of surface wettability and charge on protein adsorption onto implantable alginate‐chitosan‐alginate microcapsule surfaces

Xie¹,

Li²,

Xie³

et al. 2009

J Biomedical Materials Res

View full text Add to dashboard Cite

Alginate-chitosan-alginate (ACA) microcapsules have been developed as a device for the transplantation of living cells. However, protein adsorption onto the surface of microcapsules immediately upon their implantation decides their ultimate biocompatibility. In this work, the chemical composition of the ACA membranes was determined using X-ray photoelectron spectroscopy (XPS). The surface wettability and charge were determined by contact angle and zeta potential measurements, respectively. Then, the effects of surface wettability and charge on bovine fibrinogen (Fgn) and gamma globulin (IgG) adsorption onto ACA microcapsules were evaluated. The results showed that ACA microcapsules had a hydrophilic membrane. So, the surface wettability of ACA microcapsules had little effect on protein adsorption. There was a negative zeta potential of ACA microcapsules which varies with the viscosity or G content of alginate used, indicating a varying degree of net negatively charged groups on the surface of ACA microcapsules. The amount of adsorbed protein increased with increasing of positive charge. Furthermore, the interaction between proteins and ACA microcapsules is dominated by electrostatic repulsion at pH 7.4 and that is of electrostatic attraction at pH 6.0. This work could help to explain the bioincompatibility of ACA microcapsules and will play an important role in the optimization of the microcapsule design.

show abstract

“…There have been a number of previous reports on achieving control of the binding between the protein and the membrane by altering the membrane formulation, [4][5][6][7] including studies on the effect of polymer concentration, dissolving temperature, solvent, and addition of additives. In addition, modification of the membrane through processes such as crosslinking 8 or reactive gas plasma treatment 9 have been carried out for enhancing protein binding performance. The interaction between the membrane and protein was extensively investigated in these studies, especially with regard to protein purification and recovery.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of polyvinylidene fluoride (PVDF) membranes for protein binding: Effect of casting thickness

Ahmad

Ideris

Ooi

et al. 2012

J of Applied Polymer Sci

View full text Add to dashboard Cite

Microporous polyvinylidene fluoride (PVDF) membranes were synthesized from PVDF/N-methyl-2-pyrrolidinone (NMP) solutions using an immersion-precipitation method with a 2-propanol/water mixture as a soft coagulant. The effects of membrane thickness on pore size distribution and surface/cross-section morphology were studied using capillary flow porometry and scanning electron microscopy (SEM), respectively. All the synthesized membranes had a small range of pore size distribution, with the pore size decreasing with increasing casting thickness. The semicrystalline PVDF membranes demonstrated significant variations in morphology under SEM observation, with the existence of polymer agglomeration at a casting thickness of 500 lm and above. The protein binding capacity was observed to be highest at a casting thickness of 400 lm, where optimum pore morphology provided a large surface area for protein binding.

show abstract

Protein binding properties of surface-modified porous polyethylene membranes

Cited by 29 publications

References 58 publications

Surface Modification of Polystyrene Beads by Ultraviolet/Ozone Treatment and its Effect on Gelatin Coating

Surface Modification of Polystyrene Beads by Ultraviolet/Ozone Treatment and its Effect on Gelatin Coating

Effect of surface wettability and charge on protein adsorption onto implantable alginate‐chitosan‐alginate microcapsule surfaces

Synthesis of polyvinylidene fluoride (PVDF) membranes for protein binding: Effect of casting thickness

Contact Info

Product

Resources

About