Protein binding and biological evaluation of a polymer-anchored cobalt(iii) complex containing a 2,2′-bipyridine ligand

Abstract

The molecular interaction of polymer-anchored cobalt(III) complex, cis-[Co(bpy) 2 (BPEI)Cl]Cl 2 $4H 2 O (bpy ¼ 2,2 0 -bipyridine, BPEI ¼ branched polyethyleneimine) with two plasma proteins, human serum albumin (HSA) and bovine serum albumin (BSA) using various spectrophotomeric techniques has been investigated. The steady-state and time-resolved fluorescence spectra clearly demonstrated the static quenching mechanism. The calculated thermodynamic parameters revealed that the interaction between the polymer-cobalt(III) complex and HSA/BSA was driven mainly by van der Waals forces and hydrogen bonds. The results observed from three dimensional fluorescence and circular dichorism (CD) spectral studies manifested the conformational changes of HSA/BSA upon addition of the polymer-cobalt(III) complex. Furthermore, the antimicrobial result showed that the polymer-cobalt(III) complex exhibits good antibacterial and antifungal activities against certain human pathogenic microorganisms. In addition, the antiproliferative properties of the polymercobalt(III) complex on the HEp-2 human larynx cancer cells were determined using the MTT assay.The mode of cell death induced by the complex following treatment was analyzed adopting specific staining techniques. MTT assay revealed that the viability of the cells thus treated was significantly decreased and the cells succumbed to apoptosis as well as necrosis as reflected in changes in the nuclear morphology and cytoplasmic features by AO & EB and Hoechst staining methods.

Skimmed milk powder and pectin decorated solid lipid nanoparticle containing soluble curcumin used for the treatment of colorectal cancer

“…Hoechst staining has uncovered the deviations in cell cytology, with the unique reference to cytoplasm and core center at significant level recognizing the apoptosis. The CP‐SD‐SLN observation showed early apoptotic highlights, such as cell shrinkage, chromatin buildup, and discontinuity as seen in treated cells, and small quantities of necrotic cells (Vignesh et al, ) were observed (Figure b). After treatment with the IC 50 concentration of the SD for 24 h, the cells were viewed for cytological changes.…”

Metal-containing and related polymers for biomedical applications

“…Recently, they found that the side-chain polymer formed by the simple bipyridine ligand showed better antibacterial and antifungal activities against some human pathogenic microorganisms, such as S. aureus , Bacillus subtillis , E. coli , Pseudomonas auregenosa and Candida albicans . 160 …”

Supramolecular interaction of transition metal complexes with albumins and DNA: Spectroscopic methods of estimation of binding parameters

“…[3][4][5][6] The affinity to serum albumins has a decisive significance in the development of new medicines. [7,8] Bovine serum albumin (BSA) and human serum albumin (HSA) are the most studied proteins among other blood plasma proteins. [8] Studies of the supramolecular binding of drugs with the proteins of the albumin class are extremely important for obtaining the information on structural features of these complexes determining the therapeutic effect of drugs.…”

“…[7,8] Bovine serum albumin (BSA) and human serum albumin (HSA) are the most studied proteins among other blood plasma proteins. [8] Studies of the supramolecular binding of drugs with the proteins of the albumin class are extremely important for obtaining the information on structural features of these complexes determining the therapeutic effect of drugs. [5,6] Moreover, a great attention has lately been paid to elaboration of the methods of targeted delivery (transfer) of drugs in the human body by means of interaction of the target compound with proteins and DNA, [9,10] in particular, to tumors or inflammation nidi.…”