Protein-based nanobiosensor for direct detection of hydrogen sulfide

Omidi, Meisam; Amoabediny, Ghassem; Yazdian, Fatemeh; Habibi-Rezaei, Mehran

doi:10.1209/0295-5075/109/18005

Cited by 12 publications

(6 citation statements)

References 40 publications

(31 reference statements)

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“…LOD is defined as the lowest possible concentration of an analyte that can be distinguished without any assurance about the imprecision of the obtained results. Herein, the LOD was calculated by the following equation :

LOD = 3 σ / b

where b is the sensitivity of the sensor (slope of the regression line) and σ is the SD of the blank. Mean value and SD of Δip were calculated, since each concentration measurement was repeated three times (Figure C and D).…”

Section: Resultsmentioning

confidence: 99%

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An electrochemical biosensor for prostate cancer biomarker detection using graphene oxide–gold nanostructures

jonous

Shayeh

Yazdian

et al. 2019

Engineering in Life Sciences

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In this paper, a most sensitive electrochemical biosensor for detection of prostate‐specific antigen (PSA) was designed. To reach the goal, a sandwich type electrode composed of reduced graphene oxide/ gold nanoparticles (GO/AuNPs), Anti‐Total PSA monoclonal antibody, and anti‐Free PSA antibody was assembled. The functionalized materials were thoroughly characterized by atomic force microscope spectroscopy, transmission electron microscopy, and X‐ray diffraction techniques. The electrochemical properties of each of the modification step were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The results presented that the proposed biosensor possesses high sensitivity toward total and free PSA. Furthermore, the fabricated biosensor revealed an excellent selectivity for PSA in comparison to the other tumor markers such as BHCG, Alb, CEA, CA125, and CA19‐9. The limit of detection for the proposed electrochemical biosensor was estimated to be around 0.2 and 0.07 ng/mL for total and free PSA antigen, respectively.

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LOD = 3 σ / b

Section: Resultsmentioning

confidence: 99%

Section: Detection Of the Total And Free Psamentioning

confidence: 99%

An electrochemical biosensor for prostate cancer biomarker detection using graphene oxide–gold nanostructures

jonous

Shayeh

Yazdian

et al. 2019

Engineering in Life Sciences

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“…The k L can be described as follow :

k_{normalL} = k_{off} + k_{on} false[DOX false]

where k off is the rate of dissociation of the DOX from the GO–DOX complex and k on is the second‐order rate constant for the association of DOX and GO; the extracted values of Fig. are presented in Table 4.…”

Section: Resultsmentioning

confidence: 99%

Normalization of doxorubicin release from graphene oxide: New approach for optimization of effective parameters on drug loading

Hashemi

Yadegari

Yazdanpanah

et al. 2016

Biotech and App Biochem

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Graphene oxide (GO) has been recently introduced as a suitable anticancer drug carrier, which could be loaded with doxorubicin (DOX) as a general chemotherapy agent. Herein, the attempts were made to optimize the effective parameters on both loading and release of DOX on GO. GO and GO-DOX were characterized using transition electron microscopy , zeta potential, Raman spectroscopy, UV-visible spectroscopy, and Fourier transform infrared spectroscopy. In addition, loading and releasing behaviors of DOX on GO were studied in terms of different temperature and pH values. The primary optimized values of pH and temperature for best-loaded amount of DOX were 8.9 and 309 K, respectively. Moreover, we found that the smallest amount of released DOX, in pH of cancer microenvironment (5.4), occurs when DOX had been previously loaded in pH 7.8 and 310 K. Although the highest amount of loaded DOX was in basic pH, the results of efficient release of DOX from the GO-DOX complex and also cellular toxicity assay revealed that the best pH for loading of DOX on GO was 7.8. Therefore, in addition to optimization of parameters for efficient loading of DOX on GO, this study suggested that normalization of a released drug compared with the amount of a loaded drug could be a new approach for optimization of drug loading on nanocarriers.

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“…The PLGA MPs size was determined using an image analysis method as described previously. 13 Five different microscopy images were prepared for each sample, and the average diameters and size distributions were determined by ImageJ soware (ImageJ freeware, NIH, USA). The hydrodynamic diameter size distribution of CQDs sample was measured by Dynamic Light Scattering (DLS, Malvern ZEN 3600 zetasizer) analysis.…”

Section: Vegf Bioactivity Assaymentioning

confidence: 99%

Microfluidic synthesis of PLGA/carbon quantum dot microspheres for vascular endothelial growth factor delivery

Omidi

Hashemi

2019

RSC Adv.

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In this study, vascular endothelial growth factor (VEGF) loaded poly(D,L-lactide-co-glycolide) (PLGA)carbon quantum dot microspheres were produced using microfluidic platforms. The microcapsules were fabricated in flow-focusing geometry with a biphasic flow to generate solid/oil/water (s-o-w) droplets.To avoid any damage to protein functional and structural stability during the encapsulation process, the VEGF was PEGylated. The produced microspheres were intact and highly monodisperse in size (CV < 5%). Furthermore, microspheres in a size range of 16-36 mm were achieved by adjusting the flow ratio parameter. The encapsulation efficiency, release profile, and bioactivity of the produced microparticles were also studied. The loading efficiency of PEGylated VEGF in the microparticles was varied from 51-69% and more than 90% of PEGylated VEGF was released within 28 days. Furthermore, the release of VEGF was indirectly monitored by carbon quantum dots. The present monodisperse and controllable VEGF loaded microspheres with reproducible manner could be widely used in tissue engineering and therapeutic applications.

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Protein-based nanobiosensor for direct detection of hydrogen sulfide

Cited by 12 publications

References 40 publications

An electrochemical biosensor for prostate cancer biomarker detection using graphene oxide–gold nanostructures

An electrochemical biosensor for prostate cancer biomarker detection using graphene oxide–gold nanostructures

Normalization of doxorubicin release from graphene oxide: New approach for optimization of effective parameters on drug loading

Microfluidic synthesis of PLGA/carbon quantum dot microspheres for vascular endothelial growth factor delivery

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