Protein-Based Hydrogels

Panahi, Reza; Salehi, Mahsa Baghban

doi:10.1007/978-3-319-77830-3_52

Cited by 19 publications

(15 citation statements)

References 201 publications

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“…Biomaterials for cell culture applications are designed to mimic aspects of the ECM and provide researchers with methods to better understand cell behaviors. Protein-based hydrogels (i.e., water-swollen networks of polymers) are attractive biomaterials owing to their similarity to in vivo cellular microenvironments, biocompatibility, biodegradability and tunable mechanical properties ( Drury and Mooney, 2003 ; Panahi and Baghban-Salehi, 2019 ). Recently, we developed a bioengineered hydrogel composed of HTM cells and ECM biopolymers found in the native HTM tissue, and demonstrated its viability for investigating cell-ECM interactions under normal and simulated glaucomatous conditions in both 2D and 3D conformations ( Li et al, 2021a ; Li et al, 2021b ).…”

Section: Introductionmentioning

confidence: 99%

Extracellular Matrix Stiffness and TGFβ2 Regulate YAP/TAZ Activity in Human Trabecular Meshwork Cells

Raghunathan

Stamer

et al. 2022

Front. Cell Dev. Biol.

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Primary open-angle glaucoma progression is associated with increased human trabecular meshwork (HTM) stiffness and elevated transforming growth factor beta 2 (TGFβ2) levels in the aqueous humor. Increased transcriptional activity of Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), central players in mechanotransduction, are implicated in glaucomatous HTM cell dysfunction. Yet, the detailed mechanisms underlying YAP/TAZ modulation in HTM cells in response to alterations in extracellular matrix (ECM) stiffness and TGFβ2 levels are not well understood. Using biomimetic ECM hydrogels with tunable stiffness, here we show that increased ECM stiffness elevates YAP/TAZ nuclear localization potentially through modulating focal adhesions and cytoskeletal rearrangement. Furthermore, TGFβ2 increased nuclear YAP/TAZ in both normal and glaucomatous HTM cells, which was prevented by inhibiting extracellular-signal-regulated kinase and Rho-associated kinase signaling pathways. Filamentous (F)-actin depolymerization reversed TGFβ2-induced YAP/TAZ nuclear localization. YAP/TAZ depletion using siRNA or verteporfin decreased focal adhesions, ECM remodeling and cell contractile properties. Similarly, YAP/TAZ inactivation with verteporfin partially blocked TGFβ2-induced hydrogel contraction and stiffening. Collectively, our data provide evidence for a pathologic role of aberrant YAP/TAZ signaling in glaucomatous HTM cell dysfunction, and may help inform strategies for the development of novel multifactorial approaches to prevent progressive ocular hypertension in glaucoma.

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Section: Introductionmentioning

confidence: 99%

Extracellular Matrix Stiffness and TGFβ2 Regulate YAP/TAZ Activity in Human Trabecular Meshwork Cells

Raghunathan

Stamer

et al. 2022

Front. Cell Dev. Biol.

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“…For instance, collagen and polylysine-based hydrogels are also known. 59 However, based on their response to stimuli like pH, temperature, electric field, magnetic field and redox potential, these hydrogels can be referred to as stimuli-responsive hydrogels. 60…”

Section: Types Of Hydrogelsmentioning

confidence: 99%

Synthesis, classification and properties of hydrogels: their applications in drug delivery and agriculture

et al. 2022

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“…Rheological evaluation of the protein nanohydrogels demonstrates the impact of environmental variables such as pH, temperature, enzyme concentration, and ion concentration on rheological characteristic parameters such as viscoelastic characteristics, gelation time, gel strength, yield-strain, and various properties such as stiffness, gelation kinetics, viscoelastic regions, relative liquid–solid properties, and relaxation time scale [ 86 ]. Oscillation rheology is used to study the rheological behavior.…”

Section: Characterization Of Milk Protein Hydrogelsmentioning

confidence: 99%

Milk Protein-Based Nanohydrogels: Current Status and Applications

Kaur

Bains

Chawla

et al. 2022

Gels

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Milk proteins are excellent biomaterials for the modification and formulation of food structures as they have good nutritional value; are biodegradable and biocompatible; are regarded as safe for human consumption; possess valuable physical, chemical, and biological functionalities. Hydrogels are three-dimensional, cross-linked networks of polymers capable of absorbing large amounts of water and biological fluids without dissolving and have attained great attraction from researchers due to their small size and high efficiency. Gelation is the primary technique used to synthesize milk protein nanohydrogels, whereas the denaturation, aggregation, and gelation of proteins are of specific significance toward assembling novel nanostructures such as nanohydrogels with various possible applications. These are synthesized by either chemical cross-linking achieved through covalent bonds or physical cross-linking via noncovalent bonds. Milk-protein-based gelling systems can play a variety of functions such as in food nutrition and health, food engineering and processing, and food safety. Therefore, this review highlights the method to prepare milk protein nanohydrogel and its diverse applications in the food industry.

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Protein-Based Hydrogels

Cited by 19 publications

References 201 publications

Extracellular Matrix Stiffness and TGFβ2 Regulate YAP/TAZ Activity in Human Trabecular Meshwork Cells

Extracellular Matrix Stiffness and TGFβ2 Regulate YAP/TAZ Activity in Human Trabecular Meshwork Cells

Synthesis, classification and properties of hydrogels: their applications in drug delivery and agriculture

Milk Protein-Based Nanohydrogels: Current Status and Applications

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