Protein array profiling of mouse serum, six months post whole body radiation with 56Fe

Abstract

-To determine the chronic effects of heavy ion irradiation, an antibody based proteomic microarray technology was applied to monitor alterations in the serum proteome, six months after whole body irradiation of adult male C57Bl/6 mice with 0.5 Gray of 56 Fe. Out of 507 proteins, irradiation reduced expression of 25 proteins and enhanced expression of 12 proteins in serum (> 5% change relative to sham-irradiated controls). Of the 25 proteins found to be down-regulated, Poly ADP Ribose Polymerase (PARP) was 13% lower in the 0.5Gy mice and among the up-regulated proteins, beta-Tubulin was found to be 10% higher in the 0.5Gy group compared to the sham-irradiated 0Gy controls. Thus, irradiation with a relatively low dose of heavy ions caused persistent and selective changes in serum levels of proteins that are typically intracellular, suggesting chronic genotoxic damage.

Proteomic Analysis Implicates Dominant Alterations of RNA Metabolism and the Proteasome Pathway in the Cellular Response to Carbon-Ion Irradiation

“…A comprehensive combination of comparative proteomics and advanced bioinformatics would provide valuable insights into the complex biological networks that are influenced by radiation exposure [ 13 , 22 – 24 ]. A number of proteomic studies of heavy-ion effects have also been performed recently [ 25 – 27 ]. In the present study, a combination of proteomics and bioinformatics was applied to identify and compare the biological pathways and molecular networks involved in the response of mouse skin fibroblast cells to carbon-ion irradiation with LET values equivalent to the entrance plateau and Bragg peak.…”