Protein and peptide profiles of rats’ organs in scorpion envenomation

Gunas, V. І.; Maievskyi, Oleksandr; Raksha, Nataliia; Vovk, Tetiana; Savchuk, Olexiy; Shchypanskyi, Serhii; Гунас, И. В.

doi:10.1016/j.toxrep.2023.05.008

Cited by 5 publications

(7 citation statements)

References 38 publications

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“…And some venom peptides and/or enzymes potentially can modulate signaling pathways, affecting MMPs gene expression. The increase in MMMs content in the lungs of rats envenomed by L. macroctenus was also found in our previous studies ( Gunas et al, 2023b ). High MMMs levels are usually associated with a pro-inflammatory state, as these compounds can act as endogenous DAMPs.…”

Section: Discussionsupporting

confidence: 88%

“…Along with this, the results obtained earlier in our laboratory, showed a rise in total proteolytic activity, activities of metallo- and serine proteases, as well as an increase in matrix metalloproteinases (MMPs) and medium-mass molecules (MMMs) content in the lungs of rats envenomed by L. macroctenus , and these changes were observed starting from the 1st h with a peak at 24 h after venom injection ( Gunas et al, 2023a , 2023b ). It is known that pro-inflammatory cytokines stimulate MMPs genes expression, promoting proteolysis.…”

Section: Discussionsupporting

confidence: 64%

“…In the work, authors described imbalance in pro- and anti-inflammatory cytokines levels during envenomation with significantly increased levels of TFs HIF-1α and NF-κB, and GFs EGF, FGF-2, and VEGF, as well as IFN-γ ( Matkivska et al, 2024 ). There are limited data on this scorpion venom influence on the lungs ( Gunas et al, 2023a , 2023b ). Thus, evaluation of cytokines and their regulators content in the lungs of rats injected by L. macroctenus venom is important for studying the mechanisms of this potentially dangerous scorpion venom action on lung tissue and inflammation response involvement in these mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, ECM integrity can be violated by venom enzymes, such as hyaluronidase, as well as metallo- and serine proteases, and these mechanisms can be also involved in the modulation of growth factors production ( Ahmadi et al, 2020 ; Koç et al, 2016 ; Gunas et al, 2023b ).…”

Section: Discussionmentioning

confidence: 99%

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Cytokines and their regulators in rat lung following scorpion envenomation

Gunas,

Maievskyi,

Synelnyk

et al. 2024

Toxicon: X

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cytokines and their regulators in rat lung following scorpion envenomation

Gunas,

Maievskyi,

Synelnyk

et al. 2024

Toxicon: X

Self Cite

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“…Enzymes present in the venom enhance the process of poisoning and the spread of the venom by destroying the extracellular matrix. An increase in enzymatic activity during poisoning leads to increased tissue permeability and provides a systemic inflammatory response since poison toxins can quickly spread between all organs and tissues [8,9].…”

Section: Introductionmentioning

confidence: 99%

Peculiarities of the microscopic structure of rat testis under the influence of Vipera berus berus venom

Niyazmetov

2023

Rep. of Morph.

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Morpho-functional changes in the organs of the male reproductive system are usually associated with injuries, infectious diseases, age, lifestyle, the presence of bad habits (abuse of alcohol, drugs), the influence of environmental factors, etc. However, the action of natural toxins, in particular those that are components of animal venoms, including the venoms of snakes and vipers, is no less important. The aim of the research is to study the features of the microscopic structure of the testis of rats under the influence of Vipera berus berus venom. Experimental studies were carried out on white non-linear male rats. Animals were conditionally divided into two groups – control and experimental, 10 individuals in each. Experimental rats were injected intraperitoneally with a semi-lethal dose (LD50) (1.576 mg/g-1) of Vipera berus berus venom in physiological solution. Animals of the control group were injected intraperitoneally with only saline solution. Rats were removed from the experiment 24 hours after exposure of the venom, anesthetized by cervical dislocation. Testis samples were taken for microscopic examination. Fixation of the material and preparation of paraffin blocks were carried out according to generally accepted methods. Staining of histological preparations of the testis was carried out with hematoxylin and eosin. Histological preparations were studied using a SEO SСAN light microscope. Administration of Vipera berus berus venom to rats leads to the development of pathogistological changes in the parenchymal elements of the testis of animals, among which desquamation and disorganization of all stages of spermatogenic cells development, disruption of spermatogenesis processes were the most pronounced. Spermatogenic cells of the seminiferous tubules of the testis were distinguished by changes in morphology and location, lost the regularity of their placement, and their number decreased. The cells detached from the basement membrane and did not form a continuous layer. A characteristic feature was the presence of clusters of erythrocytes in the lumen of the seminiferous tubules, which may indicate the development of hemorrhagic complications. In addition, an increase in the number of Leydig cells was detected, which is probably the cause of changes in the hormonal regulation of the organ's function.

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Proteolytic Profile Alterations as One of the Scorpion’s <i>Leiurus macroctenus</i> Envenomation Effects on Kidneys

Ruzhena,

Serhii,

Nataliia

et al. 2024

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Scorpion envenomation becomes a serious challenge for the healthcare systems in tropical and subtropical regions. Among the variety of organs being affected by the venom, kidneys can accumulate most of the toxins recently after the sting, therefore, the homeostasis, including the proteolytic homeostasis of the renal system during the envenomation is under question. Using the SDS-PAGE and zymography methods we have investigated the proteolytic profile in the rats’ kidneys during the Leiurus macroctenus scorpion envenomation. As it turned out, envenomation leads to the increase of the relative activity of enzymes with collagenolytic, gelatinolytic and fibrinogenolytic properties with molecular weights of 30-50 kDa and 50-70 kDa, simultaneously decreasing the relative activity of proteases with higher molecular weight (70-100 kDa). We have also observed the major changes occurring in 24 hours after the envenomation. We may assume that the obtained results are most likely related either to the formation of proteolytically active fragments of proteases with higher mass, or the excessive expression of proteases with lower mass, yet additional tests need to be conducted to prove these hypotheses. Significant changes in assessed parameters in 24h after the envenomation suggest the increased danger in this period of envenomation for the proteolytic homeostasis in kidneys and the integrity of the renal system overall. Therefore, the described effects can be an important reason for the kidney dysfunctions during the Leiurus macroctenus envenomation.

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Protein and peptide profiles of rats’ organs in scorpion envenomation

Cited by 5 publications

References 38 publications

Cytokines and their regulators in rat lung following scorpion envenomation

Cytokines and their regulators in rat lung following scorpion envenomation

Peculiarities of the microscopic structure of rat testis under the influence of Vipera berus berus venom

Proteolytic Profile Alterations as One of the Scorpion’s <i>Leiurus macroctenus</i> Envenomation Effects on Kidneys

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