Protein- and Peptide-Based Biosensors in Artificial Olfaction

Barbosa, Arménio Jorge Moura; Oliveira, Ana Rita; Roque, Ana C. A.

doi:10.1016/j.tibtech.2018.07.004

Cited by 102 publications

(93 citation statements)

References 82 publications

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“…Full-size  DOI: 10.7717/peerj.8902/ fig-8 insect population management, as well as other biotechnological applications (Brito, Moreira & Melo, 2016). It was recently demonstrated that OBPs can function as molecular recognition units in gas-phase biosensors (Barbosa, Oliveira & Roque, 2018) including OBP22 of Aedes aegypti (Zhao et al, 2012). We identified 16 putative OBPs including 2 GOBPs and 3 PBPs.…”

Section: Discussionmentioning

confidence: 99%

“…ORs combine olfactory sensory neurons with binding proteins and they function in olfactory signal transduction, which also uses native sensing units to detect odors. In addition, the interactions between odorant molecules and ORs or OBPs are a source of inspiration for the design of peptides with tunable odorant selectivity (Barbosa, Oliveira & Roque, 2018;Venthur & Zhou, 2018). Insect olfactory receptors are dimers consisting of constant and variable regions (Horsfield, Haase & Turin, 2017).…”

Section: Discussionmentioning

confidence: 99%

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The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

Wang

Tao

et al. 2020

PeerJ

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Background The tiny casebearer moth Coleophora obducta, an important defoliator of Larix spp., is a major threat to ecological security in north China. Studies have shown that C. obducta is strongly specific to host plants; it is unable complete its life cycle without Larix spp. The sex pheromones of C. obducta Z5-10:OH have been elucidated; and eight types of antennae sensilla, have been detected, indicating that an exploration of its olfactory proteins is necessary, due to the general lack of information on this topic. Methods We investigated the whole body transcriptome of C. obducta, performed a phylogenetic analysis of its olfactory proteins and produced expression profiles of three pheromone-binding proteins (CobdPBPs) by qRT–PCR. Results We identified 16 odorant binding proteins, 14 chemosensory proteins, three sensory neuron membrane proteins, six odorant degrading enzymes, five antennal esterases, 13 odorant receptors, seven ionotropic receptors and 10 gustatory receptors, including three PBPs and one odorant co-receptor. Additionally, three putative pheromone receptors, two bitter gustatory receptors and five functional ionotropic receptors were found by phylogenetic analysis. The expression profiles of three PBPs in males and females showed that all of them exhibited male-specific expression and two were expressed at significantly higher levels in males. These data provide a molecular foundation from which to explore the olfactory recognition process and may be useful in the development of a new integrated pest management strategy targeting olfactory recognition of C. obducta.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

Wang

Tao

et al. 2020

PeerJ

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“…Insect OBPs, which exhibit superior stability, are assumed to have a broad molecular receptive range [42] and genetic modification has been suggested to improve their binding affinities [43]. Further description of OBPs biotechnology applications and specifically biosensing can be found in the literature [44,45]. Table 1.…”

Section: Or-based Bioelectronic Nosementioning

confidence: 99%

The Emergence of Insect Odorant Receptor-Based Biosensors

Bohbot

Vernick

2020

Biosensors

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The olfactory receptor neurons of insects and vertebrates are gated by odorant receptor (OR) proteins of which several members have been shown to exhibit remarkable sensitivity and selectivity towards volatile organic compounds of significant importance in the fields of medicine, agriculture and public health. Insect ORs offer intrinsic amplification where a single binding event is transduced into a measurable ionic current. Consequently, insect ORs have great potential as biorecognition elements in many sensor configurations. However, integrating these sensing components onto electronic transducers for the development of biosensors has been marginal due to several drawbacks, including their lipophilic nature, signal transduction mechanism and the limited number of known cognate receptor-ligand pairs. We review the current state of research in this emerging field and highlight the use of a group of indole-sensitive ORs (indolORs) from unexpected sources for the development of biosensors.

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“…Here, we focus on the main strategies that can be adopted to reach this goal ( Figure 2). More detailed reviews are available that list the sensors developed so far with this approach [8,12,13].…”

Section: Design Of Biomimetic Sensing Materialsmentioning

confidence: 99%

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Hurot

Scaramozzino

Buhot

et al. 2020

Sensors

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Artificial noses are broad-spectrum multisensors dedicated to the detection of volatile organic compounds (VOCs). Despite great recent progress, they still suffer from a lack of sensitivity and selectivity. We will review, in a systemic way, the biomimetic strategies for improving these performance criteria, including the design of sensing materials, their immobilization on the sensing surface, the sampling of VOCs, the choice of a transduction method, and the data processing. This reflection could help address new applications in domains where high-performance artificial noses are required such as public security and safety, environment, industry, or healthcare.

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Protein- and Peptide-Based Biosensors in Artificial Olfaction

Cited by 102 publications

References 82 publications

The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

The Emergence of Insect Odorant Receptor-Based Biosensors

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

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