The MASP gene family is the second most widely represented gene family in the genome of Trypanosoma cruzi. One of its main characteristics is that its 5= and 3= regions are highly conserved. We assessed the expression of these conserved regions as a marker for T. cruzi and also analyzed the expression of the masp genes and MASP proteins. In parasite strains CL-Brener (DTUVI lineage) and PAN4 (DTUI lineage), masp genes were expressed at different levels both with regard to the two strains and between stages in the parasite's life cycle. We also studied the expression of the family during the intracellular cycle of T. cruzi, using antibodies against the conserved MASP signal peptide (SP). Fluorescence intensity showed an increase in expression from 24 h onwards, with a peak in intensity at 72 h postinfection. After 24 and 48 h, the MASP proteins were expressed in 33.33% and 57.14% of the amastigotes, respectively. Our data show that not only the extracellular forms of T. cruzi but also the intracellular phases express this type of protein, though to different extents in the various forms of the parasite.
Trypanosoma cruzi is the etiological agent of American trypanosomiasis, or Chagas' disease, which affects some 16 to 18 million people in 21 countries in Central and South America. The disease has spread in recent years to countries such as the United States and Canada, and even to Europe and Australia, as a result of human migration from Latin America (24, 27). The biological cycle of T. cruzi involves hematophagous insects of the family Triatominae, in which the parasite multiplies in the epimastigote (E) form within the intestine. The infective stage, the metacyclic trypomastigote (M), develops in the insect's rectum and rectal ampolla, from whence the parasite is expelled during defecation onto the vertebrate host, upon whose blood the insect is feeding. Within the vertebrate host, the parasite goes through two phases: the trypomastigote stage (T), which can be ingested by a new insect vector or can invade any nucleated cell of the host, where it multiplies in the intracellular aflagellate amastigote (A) form, which after multiplying and occupying the cytoplasm of the cell is transformed once again into the T form. Finally, it is released to circulate through the bloodstream and invade new cells, thus completing the parasite's life cycle within the mammal (6).When the genome of T. cruzi was sequenced (12, 13), a number of multigene families were discovered spread extensively throughout the genome, including the genes encoding the MASP family of proteins (12). This is the second largest gene family (containing 1,377 genes), only 14 members of which have been identified to date, by proteomic techniques (2, 3, 14). The simultaneous expression of multiple variables was recently demonstrated after sequencing of a cDNA library of the blood trypomastigote stage, while MASP expression during the amastigote stage had already been described by the same authors (4). The function of these proteins is unknown, although the...