Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging
Ueh-Ting Tim Wang,
Xuejiao Tian,
Yae-Huei Liou
et al.
Abstract:Expansion microscopy, whereby the relative positions of biomolecules are physically increased via hydrogel expansion, can be used to reveal ultrafine structures of cells under a conventional microscope. Despite its utility for achieving super-resolution imaging, expansion microscopy suffers a major drawback, namely reduced fluorescence signals caused by excessive proteolysis and swelling effects. This caveat results in a lower photon budget and disfavors fluorescence imaging over a large field of view that can… Show more
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