Protein adsorptive behavior on mesoporous titanium dioxide determined by geometrical topography

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

“…Imaging and qualitative detection of proteins onto a surface can be also performed by atomic forces microscopy (AFM) [ 58 , 110 , 143 ]. It is possible to image single proteins or agglomerates on the surface [ 58 ] or, thanks to appropriate tip modifications, it is also possible to measure the interaction forces between the proteins and surfaces [ 82 ]. Distribution of proteins onto a surface can be imaged thanks to the use of confocal laser scanning microscopy (CLSM) coupled with use of fluorescent-labeled proteins [ 95 , 100 ].…”

“…In the case of BSA, of which hydrodynamic diameter is about 7.2 nm, mesopores need to be at least about 9 nm for albumin to enter them [ 81 ]. Larger mesopores can accommodate more than one BSA molecule, with very little conformational changes, and protein-surface adhesion forces were stronger with respect to smaller pores [ 82 ]. Singh et al [ 83 ], due to protein tendency to aggregate into nanopores, concluded that nanometer scale morphology is the main reason for increased protein adsorption, more than the modest increase in wettability of surface with different roughness.…”

Excellent Protein Immobilization and Stability on Heterogeneous C–TiO2 Hybrid Nanostructures: A Single Protein AFM Study

“…It has been found that surface chemical modifications, such as covalent bonding, cross-linking, and grafting, can be used to increase the immobilization and stability of the biomolecules. , However, some unexpected side effects such as weakening of the biomolecule response or irreversible deactivation may occur . Previous research has shown that the pore structure of the TiO 2 surface is highly important for the immobilization and stability of biomolecules. ,, Therefore, surface modification may weaken its positive effect. Hence, a surface modification that enhances stability while maintaining the geometric properties of TiO 2 is highly desirable.…”

Enhancement of operational stability of chloroperoxidase from Caldariomyces fumago by immobilization onto mesoporous supports and the use of co-solvents

“…This material has been widely used as supports in Au catalysts for heterogeneous catalysis of alkene epoxidation [26] and as an electrode modifier for horseradish peroxidase (HRP) immobilization for electrochemical biosensors [27][28][29][30][31]. Recently, TiO 2 nanoparticles were used for HRP immobilization by physical adsorption [32], and the relationship between the geometric topography of the mesopores of TiO 2 and adsorption of some proteins (bovine serum albumin, myoglobin and lysozyme) at their isoelectric point has been studied [33].…”