Protein adsorption through Chitosan–Alginate membranes for potential applications

Abstract

BackgroundChitosan and Alginate were used as biopolymers to prepare membranes for protein adsorption. The network requires a cross-linker able to form bridges between polymeric chains. Viscopearl-mini® (VM) was used as a support to synthesize them. Six different types of membranes were prepared using the main compounds of the matrix: VM, Chitosan of low and medium molecular weight, and Alginate.ResultsExperiments were carried out to analyze the interactions within the matrix and improvements were found against porous cellulose beads. SEM characterization showed dispersion in the compounds. According to TGA, thermal behaviour remains similar for all compounds. Mechanical tests demonstrate the modulus of the composites increases for all samples, with major impact on materials containing VM. The adsorption capacity results showed that with the removal of globular protein, as the adsorbed amount increased, the adsorption percentage of Myoglobin from Horse Heart (MHH) decreased. Molecular electrostatic potential studies of Chitosan–Alginate have been performed by density functional theory (DFT) and ONIOM calculations (Our own N-layered integrated molecular orbital and molecular mechanics) which model large molecules by defining two or three layers within the structure that are treated at different levels of accuracy, at B3LYP/6-31G(d) and PM6/6-31G(d) level of theory, using PCM (polarizable continuum model) solvation model.ConclusionsFinally, Viscopearl-mini® acts as a suitable support on the matrix for the synthesis of Chitosan–Alginate membranes instead of cross-linkers usage. Therefore, it suggests that it is a promise material for potential applications, such as: biomedical, wastewater treatment, among others.Graphical abstractChitosan, Alginate, and Cellulose beads-based membranes for protein adsorption. Special attention was given for preparation, charaterization, adsorption capacity, and molecular electrostatic potential studies calculation. Viscopearl-mini® gives support on the matrix of Chitosan–Alginate membranes instead of cross-linkers usage

Adsorption Kinetics of Cellulase and Xylanase Immobilized on Magnetic Mesoporous Silica

“…The third step resembles the equilibrium of the adsorption process [44]. These findings corroborate the ideas of Murguia-Flores et al [47] and Viegas et al [48], who suggested that the rate-limiting step was not only concerned with intraparticle diffusion but was also related to the participation of film diffusion in the adsorption mechanism of the enzymes. The second and the third stage as mentioned above are occupied by the intraparticle diffusion process [45].…”

Characterization of Sodium Alginate Hydrogels Reinforced with Nanoparticles of Hydroxyapatite for Biomedical Applications

“…Sodium alginate (SA) is a common hydrogel polymer mainly extracted from brown seaweed or bacterial sources [4,5]. It is biodegradable and highly biocompatible and it is employed for tissue engineering purposes, as well as for protein and drug release [6][7][8][9]. Alginate is now known to be a whole family of linear copolymers containing blocks of (1,4)-linked β-D-mannuronate (M) and α-L-guluronate (G) residues.…”

Sodium alginate/chitosan/glyphosate superabsorbent bio‐foam as a release system for herbicide

“…A stock solution of 100 mgÁL À1 of glyphosate was used to prepare six solutions containing different concentrations of the herbicide (12,20,28,36,44, and 52 mgÁL À1 ) and placed in test tubes. In each tube, 1 ml of ninhydrin (5% m/v) and 1 ml of sodium molybdate (5% m/v) were added.…”

“…26,27 In the literature, some works showed the preparation of SA and CH membranes to be employed in adsorption and removal of glyphosate from water sources. 10,16,28 Moreover, Silva et al 27 studied nanoparticles formed during ionotropic gelification as a carrier for the herbicide paraquat. Isiklan 29 prepared SA/gelatin and SA/sodium carboxymethyl cellulose blend beads crosslinked with glutaraldehyde to be applied in controlled release of carbaryl insecticide.…”