Protein adsorption properties of OEG monolayers and dense PNIPAM brushes probed by neutron reflectivity

Brouette, Nicolas; Xue, Changying; Haertlein, Michael; Moulin, Martine; Fragneto, Giovanna; Leckband, Deborah; Halperin, A.; Sferrazza, Michele

doi:10.1140/epjst/e2012-01681-4

Cited by 11 publications

(19 citation statements)

References 39 publications

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“…For measurements in water the sample was placed in a stainless steel cell filled with MilliQ water and mounted on top of the sample stage. Here For PNIPAM-B-0.2-240 at 15 C we assumed a four-layer model, referring to earlier results by Brouette et al 35 We justify this by the comparable brush samples. In contrast to the above mentioned three-layer model an additional brush layer towards the D 2 O layer with a higher solvent content is added.…”

Section: Ellipsometrymentioning

confidence: 93%

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Grazing Incidence Neutron Spin Echo Study of Poly(N-isopropylacrylamide) Brushes

et al. 2020

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The structure and the dynamic properties of poly(N -isopropylacrylamide) brushes at solid planar surfaces under good solvent conditions are studied. Polymer brushes with grafting densities in the high density and semidilute regime are prepared via surfaceinitiated atom transfer radical polymerization. The polymer brushes were removed from the substrates to determine the molar mass and molecular dispersity via size exclusion chromatography to draw conclusions about the grafting density. The structure of the brushes was investigated with ellipsometry and neutron reflectometry measurements.We find an influence of the grafting density on the swelling properties of the poly(Nisopropylacrylamide) brushes. Furthermore, the brush dynamics at different neutron † A footnote for the title 1 penetration depths was analyzed with neutron spin echo spectroscopy under grazing incidence. The evanescent intensity distribution was modeled with the BornAgain software package, which uses the Distorted Wave Born Approximation.

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Section: Ellipsometrymentioning

confidence: 93%

“…For PNIPAM-B-0.2-240 at 15 °C, we assumed a four-layer model, referring to earlier results obtained by Brouette et al We justify this by the comparable brush samples. In contrast to the above-mentioned three-layer model, an additional brush layer toward the D 2 O layer with a higher solvent content is added.…”

Section: Methodsmentioning

confidence: 99%

Grazing Incidence Neutron Spin Echo Study of Poly(N-isopropylacrylamide) Brushes

et al. 2020

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“…The density of dry PNIPAM, , is sometimes arbitrarily taken equal to 0.95 g/cm 3 by certain authors [106], but the actual density can be measured by U-tube oscillation [107] or by X-ray reflectometry [108]. Fourier transform infrared (FTIR) spectroscopy can also be used, in some cases, to determine the graft density, as reported by Mizutani et al [109].…”

Section: Characterization Of Brushesmentioning

confidence: 99%

PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion

Conzatti

Cavalié

Combes

et al. 2017

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tBiomaterials surface design is critical for the control of materials and biological system interactions. Being regulated by a layer of molecular dimensions, bioadhesion could be effectively tailored by polymer surface grafting. Basically, this surface modification can be controlled by radical polymerization, which is a useful tool for this purpose. The aim of this review is to provide a comprehensive overview of the role of surface characteristics on bioadhesion properties. We place a particular focus on biomaterials functionalized with a brush surface, on presentation of grafting techniques for "grafting to" and "grafting from" strategies and on brush characterization methods. Since atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT) polymerization are the most frequently used grafting techniques, their main characteristics will be explained. Through the example of poly(N-isopropylacrylamide) (PNIPAM) which is a widely used polymer allowing tuneable cell adhesion, smart surfaces involving PNIPAM will be presented with their main modern applications.

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“…In thicker brushes, the changing signal of the top layer would be overlapped by the less-changing signal of the underlying bulk layer. 15 Moreover, brush parameters like the grafting density are easily adjustable in the preparation of ultrathin brushes, which in the future might be favorable for tuning the proteinadsorbing properties.…”

Section: Introductionmentioning

confidence: 99%

In Situ Infrared Ellipsometry for Protein Adsorption Studies on Ultrathin Smart Polymer Brushes in Aqueous Environment

Kroning

Furchner

Aulich

et al. 2015

ACS Appl. Mater. Interfaces

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The protein-adsorbing and -repelling properties of various smart nanometer-thin polymer brushes containing poly(N-isopropylacrylamide) and poly(acrylic acid) with high potential for biosensing and biomedical applications are studied by in situ infrared-spectroscopic ellipsometry (IRSE). IRSE is a highly sensitive nondestructive technique that allows protein adsorption on polymer brushes to be investigated in an aqueous environment as external stimuli, such as temperature and pH, are varied. These changes are relevant to conditions for regulation of protein adsorption and desorption for biotechnology, biocatalysis, and bioanalytical applications. Here brushes are used as model surfaces for controlling protein adsorption of human serum albumin and human fibrinogen. The important finding of this work is that IRSE in the in situ experiments in protein solutions can distinguish between contributions of polymer brushes and proteins. The vibrational bands of the polymers provide insights into the hydration state of the brushes, whereas the protein-specific amide bands are related to changes of the protein secondary structure.

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Protein adsorption properties of OEG monolayers and dense PNIPAM brushes probed by neutron reflectivity

Cited by 11 publications

References 39 publications

Grazing Incidence Neutron Spin Echo Study of Poly(N-isopropylacrylamide) Brushes

Grazing Incidence Neutron Spin Echo Study of Poly(N-isopropylacrylamide) Brushes

PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion

In Situ Infrared Ellipsometry for Protein Adsorption Studies on Ultrathin Smart Polymer Brushes in Aqueous Environment

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