Protein adsorption on thermoplastic elastomeric surfaces: A quantitative mass spectrometry study

Casiano-Maldonado, Madalis; Lim, G. T.; Li, Xiaopeng; Reneker, Darrell H.; Puskás, Judit E.; Wesdemiotis, Chrys

doi:10.1016/j.ijms.2013.08.007

Cited by 8 publications

(8 citation statements)

References 31 publications

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“…Subsequently, the degradation products, in this case individual amino acids, can be quantified 119. 120 Similarly, mass spectrometric analysis of adsorbed proteins121 can also be performed after tryptic digestion, in which a fragment analysis may allow the detection of the adsorbed proteins from complex mixtures 122…”

Section: Methods For the Analysis Of Protein Interactionsmentioning

confidence: 99%

Protein Interactions with Polymer Coatings and Biomaterials

et al. 2014

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Protein adsorption is considered to be the most important factor of the interaction between polymeric biomaterials and body fluids or tissues. Water-mediated hydrophobic and hydration forces as well as electrostatic interactions are believed to be the major factors of protein adsorption. A systematic analysis of various monolayer systems has resulted in general guidelines, the so-called "Whitesides rules". These concepts have been successfully applied for designing various protein-resistant surfaces and are being studied to expand the understanding of protein-material interactions beyond existing limitations. Theories on the mechanisms of protein adsorption are constantly being improved due to the fast-developing analytical technologies. This Review is aimed at improving these empirical guidelines with regard to present theoretical and analytical advances. Current analytical methods to test mechanistic hypotheses and theories of protein-surface interactions will be discussed. Special focus will be given to state-of-the-art bioinert and biospecific coatings and their applications in biomedicine.

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Section: Methods For the Analysis Of Protein Interactionsmentioning

confidence: 99%

Protein Interactions with Polymer Coatings and Biomaterials

et al. 2014

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“…10,11 Thermoplastic polyurethanes (TPU) are well established for use in biomedical applications because of their high bio and blood compatibility, and owing to their efficient adaptability to mechanical forces, both of which are properties that are similar to natural tissues. 15 We present for the first time results from in vivo experiments and moreover not only for proteins but also lipids. 12,13 However, thrombus formation is a crucial limitation for these materials and the interactions between polymer materials and the biological environment has not been described in detail, even though it clearly impacts material modification and improvement.…”

Section: Introductionmentioning

confidence: 97%

“…14 To our knowledge only one study is available showing the impact of polymer surface and structure, pH of the environment and molecular weights and pIs of the model proteins on their adsorption on thermoplastic elastomeric surfaces. 15 We present for the first time results from in vivo experiments and moreover not only for proteins but also lipids.…”

Section: Introductionmentioning

confidence: 97%

Mass spectrometric imaging of in vivo protein and lipid adsorption on biodegradable vascular replacement systems

Fröhlich

Eilenberg

Svirkova

et al. 2015

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Cardiovascular diseases present amongst the highest mortality risks in Western civilization and are frequently caused by arteriosclerotic vessel failure. Coronary artery and peripheral vessel reconstruction necessitates the use of small diameter systems that are mechanically stress-resistant and biocompatible. Expanded polytetrafluorethylene (ePTFE) is amongst the materials used most frequently for non-degradable and bio-degradable vessel reconstruction procedures, with thermoplastic polyurethanes (TPU) representing a promising substitute. The present study describes and compares the biological adsorption and diffusion occurring with both materials following implantation in rat models. Gel electrophoresis and thin-layer chromatography, combined with mass spectrometry and mass spectrometry imaging, were utilized to identify the adsorbed lipids and proteins. The results were compared with the analytes present in native aorta tissue. It was revealed that both polymers were severely affected by biological adsorption after 10 min in vivo. Proteins associated with cell growth and migration were identified, especially on the luminal graft surface, while lipids were found to be located on both the luminal and abluminal surfaces. Lipid adsorption and cholesterol diffusion were found to be correlated with the polymer modifications identified on degradable thermoplastic urethane graft samples, with the latter revealing extensive cholesterol adsorption. The present study demonstrates an interaction between biological matter and both graft materials, and provides insights into polymer changes, in particular, those observed with thermoplastic urethanes already after 10 min in vivo exposure. ePTFE demonstrated minor polymer modifications, whereas several different polymer signals were observed for TPU, all were co-localized with biological signals.

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“…Nachfolgend können dann die Abbauprodukte, in diesem Fall die einzelnen Aminosäuren, quantifiziert werden 119. 120 Des Weiteren kann auch eine massenspektrometrische Analyse der adsorbierten Proteine121 im Anschluss an einen tryptischen Verdau durchgeführt werden, wobei die Fragmentanalyse den Nachweis adsorbierter Proteine auch in komplexen Mischungen erlauben kann 122…”

Section: Methoden Für Die Analyse Von Proteinwechselwirkungenunclassified

Wechselwirkungen von Proteinen mit Polymerbeschichtungen und Biomaterialien

et al. 2014

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Die Proteinadsorption gilt als der wichtigste Faktor der Wechselwirkung zwischen polymeren Biomaterialien und Körperflüssigkeiten oder ‐gewebe. Die Haupteinflussfaktoren auf die Proteinadsorption sind wasservermittelte hydrophobe und Hydratationskräfte sowie elektrostatische Wechselwirkungen. Eine systematische Analyse verschiedener Monolagen führte zur Aufstellung allgemeiner Leitsätze, den sogenannten “Whitesides‐Regeln”. Diese Konzepte wurden erfolgreich auf die Entwicklung verschiedener proteinresistenter Oberflächen angewendet und werden kontinuierlich weiterentwickelt, um das Verständnis von Protein‐Material‐Wechselwirkungen über die bisherigen Grenzen hinaus zu erweitern. Ebenso werden die Theorien zu Proteinadsorptionsmechanismen aufgrund der sich schnell entwickelnden analytischen Technologien fortlaufend verbessert. Ziel dieses Aufsatzes ist die Verbesserung der aufgestellten empirischen Leitlinien im Hinblick auf die theoretischen und analytischen Fortschritte. Dabei werden die aktuellen analytischen Methoden zur Untersuchung mechanistischer Hypothesen und Theorien zu Protein‐Oberflächen‐Wechselwirkungen besprochen. Ein besonderes Augenmerk liegt auf aktuellen Technologien im Bereich bioinerter und biospezifischer Beschichtungen und ihrer Anwendungen in der Biomedizin.

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Protein adsorption on thermoplastic elastomeric surfaces: A quantitative mass spectrometry study

Cited by 8 publications

References 31 publications

Protein Interactions with Polymer Coatings and Biomaterials

Protein Interactions with Polymer Coatings and Biomaterials

Mass spectrometric imaging of in vivo protein and lipid adsorption on biodegradable vascular replacement systems

Wechselwirkungen von Proteinen mit Polymerbeschichtungen und Biomaterialien

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