Protein adsorption on clay minerals

Jaber, Maguy; Lambert, Jean‐François; Balme, Sébastien

doi:10.1016/b978-0-08-102432-4.00008-1

Cited by 12 publications

(3 citation statements)

References 118 publications

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“…29,30 It has also been determined that the energy necessary to unfold an α-helix is smaller than the energy needed to unfold a β-sheet. 31,32 Thus, soft proteins are expected to have a higher α-helix/β-sheet ratio than hard proteins. In wine, chitinases and β-glucanase are the first to adsorb on bentonite.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Hard proteins have since been described as having an unfolding energy higher than soft proteins, e.g., 60 and 21 kJ mol –1 , respectively. , It has also been determined that the energy necessary to unfold an α-helix is smaller than the energy needed to unfold a β-sheet. , Thus, soft proteins are expected to have a higher α-helix/β-sheet ratio than hard proteins.…”

Section: Resultsmentioning

confidence: 99%

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Wine Thermosensitive Proteins Adsorb First and Better on Bentonite during Fining: Practical Implications and Proposition of Alternative Heat Tests

Vernhet

Meistermann

Cottereau

et al. 2020

J. Agric. Food Chem.

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Bentonite fining is the most popular treatment used to remove proteins in white and rosé wines. The usual heat test used to adjust the bentonite dose consists of heating the wine during 30 min at 80 °C. At this temperature, all of the proteins are unfolded, and this can lead to an overestimation of the dose. We have shown that proteins adsorb on bentonite in a specific order and, more importantly, that the proteins responsible for haze formation adsorb first. Fluorescence spectroscopy showed that this is due to the structural properties of proteins, which can be classified as hard and soft proteins. Alternative heat tests were performed at a lower temperature (40 °C) and showed a better correlation with accelerated aging. These tests were also less dependent upon the wine pH.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Wine Thermosensitive Proteins Adsorb First and Better on Bentonite during Fining: Practical Implications and Proposition of Alternative Heat Tests

Vernhet

Meistermann

Cottereau

et al. 2020

J. Agric. Food Chem.

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“…While 2D silicate nanoclays have been widely used for the adsorption and delivery of small molecules, much less research has been devoted to macromolecules such as proteins and nucleic acids. The larger size of these macromolecules may lead to varying interactions with nanoclays compared to small molecules [ 129 ]. Proteins have been shown to form relatively large complexes with Laponite, with the size of these complexes being contingent on protein charge and nanoclay concentration [ 39 , 69 ].…”

Section: Laponite Composite Hydrogels For Delivery Of Macromoleculesmentioning

confidence: 99%

Laponite-Based Nanocomposite Hydrogels for Drug Delivery Applications

2023

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Hydrogels are widely used for therapeutic delivery applications due to their biocompatibility, biodegradability, and ability to control release kinetics by tuning swelling and mechanical properties. However, their clinical utility is hampered by unfavorable pharmacokinetic properties, including high initial burst release and difficulty in achieving prolonged release, especially for small molecules (<500 Da). The incorporation of nanomaterials within hydrogels has emerged as viable option as a method to trap therapeutics within the hydrogel and sustain release kinetics. Specifically, two-dimensional nanosilicate particles offer a plethora of beneficial characteristics, including dually charged surfaces, degradability, and enhanced mechanical properties within hydrogels. The nanosilicate–hydrogel composite system offers benefits not obtainable by just one component, highlighting the need for detail characterization of these nanocomposite hydrogels. This review focuses on Laponite, a disc-shaped nanosilicate with diameter of 30 nm and thickness of 1 nm. The benefits of using Laponite within hydrogels are explored, as well as examples of Laponite–hydrogel composites currently being investigated for their ability to prolong the release of small molecules and macromolecules such as proteins. Future work will further characterize the interplay between nanosilicates, hydrogel polymer, and encapsulated therapeutics, and how each of these components affect release kinetics and mechanical properties.

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