Protein adsorption/desorption and antibody binding stoichiometry on silicon interferometric biosensors examined with TOF-SIMS

Abstract

Highlights  Examining microstructured sensing areas of interferometric biosensors on silicon chip  Step-by-step surface modifications determined with biomolecular discrimination  Surface density of probe, blocking protein, primary and secondary antibody evaluated  Desorption upon primary immunoreaction depends on spotting concentration of probe  Binding stoichiometry evaluated more accurately than estimated from sensor response

Biological responses to physicochemical properties of biomaterial surface

“…Owing to the advances in analytical chemistry, biomedical researchers have achieved in depth information about the surface composition and structure of both biomaterials and biological molecules. [428][429][430] The commonly used analytical techniques for biomaterial surface characterization include secondary-ion mass spectrometry (SIMS), time of flight-SIMS (ToF-SIMS), X-ray photoelectron spectroscopy (XPS), auger electron spectroscopy (AES), (Raman), infrared (IR) spectroscopy, X-ray scattering and spectroscopy techniques, scanning electron microscopy-energy dispersive X-ray (SEM-EDX), atomic force microscopy (AFM) and streaming potential measurements/ electroosmosis. [431][432][433][434] Among these techniques, XPS, ToF-SIMS, Raman, and IR spectroscopy have gained substantial attention for characterizing the surface composition after applying surface treatments.…”

“…Analytical chemistry can also be useful to provide information about the chemical characterization of surface bound proteins. 430,433,435 Proteins are recognized as key players in determining biomaterial-cell interactions and many biological pathways. 14 The protein structure, which is the 3D arrangement of atoms in an amino acid-chain, can break down into primary structural units, secondary structural units, tertiary structural units, and quaternary structural units.…”

Study of a Broadband Difference Interferometer Based on Low-Cost Polymer Slab Waveguides

“…A description of the broadband Mach–Zehnder interferometer can be found in the literature [16,17]. The specific applications of this interferometric system can be found in many publications [18,19,20,21,22,23,24,25,26].…”

Using a lactadherin-immobilized silicon surface for capturing and monitoring plasma microvesicles as a foundation for diagnostic device development

“…different proteins [ 27 , 28 ] and lipids [ 29 – 31 ], based on the differences in their chemical structure, and for comparison of surface molecular composition [ 32 ]. Therefore, ToF-SIMS is an excellent method for examination of surface functionalization involving multistep and multimolecular protocols of biosensor surface functionalization [ 33 , 34 ]. A comparison of the intensities of secondary ion fragments chosen as characteristics for the substrate, all proteins, LACT, and particular lipids is presented in Fig.…”