Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses

Andrade, Joseph D.; Hlady, Vladimir

doi:10.1007/3-540-16422-7_6

Cited by 798 publications

(375 citation statements)

References 105 publications

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“…Adherent cells then slowly (typically within hours) spread over the surface, depending on compatibility with the surface, expressing a strong 'biological component of adhesion' [38] that includes secretion of extracellular matrix (ECM) and results in the flattening of cells on the substratum [2] (spreadcell length is about 3-10 times height [9]). Needless to say, (protein) composition of the fluid phase can greatly affect the entire cell adhesion process [31,33,[55][56][57][58][59]. Thus, it is apparent that the short-term (<3 days) cell adhesion/proliferation process spans a broad range of time and length scales.…”

Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (α 2 , α 3 , α 4 , α 5 , α v , β 1 and β 3 ) integrins, vinculin, as well as secretion of osteopontin and type I collagen supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events -contact, attachment, spreading, and proliferation -are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum compatibility-superposition-principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cellsubstratum compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause-and-effect relationships between cell cell-substratum compatibility and substratum properties.Impact Statement-This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell

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Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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“…Spontaneous movement of water and ions to accommodate the energetic perturbation induced by creation of the interface formed by bringing protein solution into contact with a physical surface [65]. In this work, hydrophobic buffer/air or buffer/ODS adsorbent-particle interfaces are specifically considered, wherein the buffer contains varying concentrations of the purified proteins listed in Varoqui and Pefferkorn [13].…”

Section: Time-dependent Interphase Volumementioning

confidence: 99%

“…A working hypothesis that has emerged as a fundamental biomaterials-surface-science tenet from more than three decades of biomaterials research is that protein adsorption is among the first steps [24] in the acute biological response to artificial materials [5,65,[74][75][76][77]. Hence, biocompatibility is presumed to be directly related to protein adsorption.…”

Section: Implications For the Biological Response To Materialsmentioning

confidence: 99%

Volumetric interpretation of protein adsorption: Kinetic consequences of a slowly-concentrating interphase

Barnthip¹,

Noh²,

Leibner³

et al. 2008

Biomaterials

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Time-dependent energetics of blood-protein adsorption are interpreted in terms of a slowlyconcentrating three-dimensional interphase volume initially formed by rapid diffusion of protein molecules into an interfacial region spontaneously formed by bringing a protein solution into contact with a physical surface. This modification of standard adsorption theory is motivated by the experimental observation that interfacial tensions of protein-containing solutions decrease slowly over the first hour to a steady-state value while, over this same period, the total adsorbed-protein mass is constant (for lysozyme, 15 kDa; albumin, 66 kDa; prothrombin, 72 kDa; IgG, 160 kDa; fibrinogen, 341 kDa studied in this work). These seemingly divergent observations are rationalized by the fact that interfacial energetics (tensions) are explicit functions of solute chemical potential (concentration), not adsorbed mass. Hence, rates-of-interfacial-tension-change parallel a slow interphase concentration effect whereas solution depletion detects a constant interphase composition within the time frame of experiment. A straightforward mathematical model approximating the perceived physical situation leads to an analytic formulation that is used to compute time-varying interphase volume and protein concentration from experimentally-measured interfacial tensions. Derivation from the fundamental thermodynamic adsorption equation verifies that protein adsorption from dilute solution is controlled by a partition coefficient at equilibrium, as is observed experimentally at steady state. Implications of the alternative interpretation of adsorption kinetics on biomaterials and biocompatibility are discussed. KeywordsProtein adsorption; kinetics; interfacial energetics; interphase; surface; radiometry IntroductionProtein-adsorption kinetics are of practical importance in biomaterials because adsorption rates have been implicated as a cause of selective protein adsorption. For example, the so-called Vroman Effect is commonly thought to occur because low-molecular-weight proteins *Author to whom correspondence should be addressed EAV3@PSU.EDU. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptBiomaterials. Author manuscript; available in PMC 2009 July 1. Published in final edited form as:Biomaterials. presumably arriving first at a surface immersed in a multi-component solution are displaced by higher-molecular-weight proteins arriving later (see refs. and citations therein). The final adsorbed-protein composition is thus purported to be achieved through a complex se...

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“…[1][2][3] The first biological event which occurs on the material surface when biomaterials are either studied in vitro or implanted in vivo is protein adsorption from culture media or physiological fluids respectively. [4][5][6] Differences in protein absorption and configuration on the substrate play a pivotal role in cellular behaviour; including cell adhesion, proliferation, migration and differentiation. [7][8][9] Integrins are the major receptors for proteins adsorbed on material surface and trigger cell adhesion and activate diverse intracellular signalling pathways to regulate cell fate.…”

Section: Introductionmentioning

confidence: 99%

Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

Mnatsakanyan

Rico

Grigoriou

et al. 2015

ACS Appl. Mater. Interfaces

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Fibronectin fibrillogenesis is the physiological process by which cells elaborate a fibrous FN matrix. Poly(ethyl acrylate), PEA, has been described to induce a similar process upon simple adsorption of fibronectin (FN) from a protein solution -in the absence of cells -leading to the so-called material-driven fibronectin fibrillogenesis. Poly(methyl acrylate), PMA, is a polymer with very similar chemistry to PEA, on which FN is adsorbed keeping the globular conformation of the protein in solution. We have used radical polymerisation to synthesise copolymers with controlled EA/MA ratio seeking to modulate the degree of FN fibrillogenesis. The physico-chemical properties of the system were studied using dynamicmechanical analysis, differential scanning calorimetry and water contact angle. Both the degree of FN fibrillogenesis and the availability of the integrin binding region of FN directly depend on the percentage of EA in the copolymer, whereas the same total amount of FN was adsorbed regardless the EA/MA ratio. Cell morphology adhesion and differentiation of murine C2C12 were shown to depend on the degree of FN fibrillogenesis previously attained on the material surface. Myogenic differentiation was enhanced on the copolymers with higher EA content, i.e. more interconnected FN fibrils.

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Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses

Cited by 798 publications

References 105 publications

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Volumetric interpretation of protein adsorption: Kinetic consequences of a slowly-concentrating interphase

Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

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