Protein A Reactivity of Various Mammalian Immunoglobulins

Goudswaard, J.; Donk, J.A. van der; Noordzij, A.; Dam, R.H. Van; Vaerman, Jean‐Pierre

doi:10.1111/j.1365-3083.1978.tb00492.x

Cited by 286 publications

(76 citation statements)

References 9 publications

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“…Binding of antibodies to the protein A moiety was first demonstrated on purified particles by using immunogold labeling. Because protein A binds efficiently to the constant regions of many subclasses of IgG from different species (19), this test could be done either directly by using any gold-conjugated antibody or indirectly by using a primary unlabeled and a secondary gold-conjugated antibody. Although the first approach already gave good results (data not shown), these were further improved by including a secondary antibody.…”

Section: Cp-protein a Fusion Is Expressed At A High Level In Plant Timentioning

confidence: 99%

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A

Werner¹,

Marillonnet²,

Hause³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

122

123

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Earlier attempts to express peptides longer than 20 aa on the surface of tobamoviruses such as tobacco mosaic virus have failed. Surprisingly, we found that a functional fragment of protein A (133 aa) can be displayed on the surface of a tobamovirus as a Cterminal fusion to the coat protein via a 15-aa linker. The macromolecular nature of these nanoparticles allowed the design of a simple protocol for purification of mAbs with a recovery yield of 50% and >90% product purity. The extremely dense packing of protein A on the nanoparticles (>2,100 copies per viral particle) results in an immunoadsorbent with a binding capacity of 2 g mAb per g. This characteristic, combined with the high level of expression of the nanoparticles (>3 g adsorbent per kg of leaf biomass), provides a very inexpensive self-assembling matrix that could meet the criteria for a single-use industrial immunoadsorbent for antibody purification.antibody purification ͉ coat protein fusion ͉ viral particle

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Section: Cp-protein a Fusion Is Expressed At A High Level In Plant Timentioning

confidence: 99%

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A

Werner¹,

Marillonnet²,

Hause³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

122

123

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“…Rabbit antibody against G6PD was prepared as described by DeFlora et al (1977). Anti-G6PD IgG was purified from rabbit serum by (NH&SO, precipitation, DE-52 cellulose column chromatography (Weir, 1967), and affinity chromatography on a protein A-Sepharose CL-4B column (Goudswaard et al, 1978). Heparin (20 u/ml) was added to the antibody preparation to inhibit RNase activity.…”

Section: (A) Purification Of Glucosed-phosphate Dehydrogenase and Prementioning

confidence: 99%

Isolation and characterization of the glucose-6-phosphate dehydrogenase gene of Drosophila melanogaster

Ganguly

Manning

1985

Gene

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“…Rabbit antisera could be used after simple affinity chromatography with protein A (Goudswaard et al, 1978 …”

Section: Cerebrospinal Fluidmentioning

confidence: 99%

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

Goudswaard

Verdouw-Chamalaun

Noordzij

1980

Veterinary Immunology and Immunopathology

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In twenty-five ovine body fluids (serum, lung fluid and cerebrospinal fluid), the concentrations of IgGl, IgG2, IgM and IgA were determined by laser nephelometry and radial immunodiffusion (RID). When nephelometric assays are carried out, antisera free from any turbidity are essential. Methods ensuring that goat and rabbit antisera will satisfy this requirement are described in the present paper. When sheep immunoglobulins were measured by laser nephelometry, adequate and reproducible results were obtained, comparable with those obtained by RID.Advantages of the nephelometric method include the speed of assay and its sensitivity, allowing precise determination of the very low concentrations of immunoglobulins in CSF.

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Protein A Reactivity of Various Mammalian Immunoglobulins

Cited by 286 publications

References 9 publications

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A

Isolation and characterization of the glucose-6-phosphate dehydrogenase gene of Drosophila melanogaster

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

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