Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

Qiu, Lin; Bi, Yanhua; Wang, Cheli; Li, Jingyan; Guo, Peilin; Jin-chen, LI; He, Weijiang; Wang, Jianhao; Jiang, Pengju

doi:10.3390/ijms15021804

Cited by 14 publications

(9 citation statements)

References 29 publications

(29 reference statements)

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“…With these advantages, the QDFM have potential as a new labeling technology immunoassay [37,38]. The microspheres ampli ed the optical signal of speci c antigen-antibody binding and the sensitivity is improved compared with other labelled technologies [39,40]. This method avoids shortcomings of time consumption and the use of accurate and expensive instruments, with advantages of rapid on-site detection, low cost, and no special operation requirements [41,42].…”

Section: Discussionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

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Background: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic test strip (ICTS) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum.Result: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The detection results were achieved by using the ratio of the fluorescence signals of the test and control lines (HT/HC) with a threshold of 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICTS and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, and no cross reaction with the sera of other related diseases was observed.Conclusion: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost.

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Section: Discussionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

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“…Optical detection in association with nanomaterials is mainly connected with quantum dots due to their application as a fluorescent labels in laser-induced fluorescence detection [62][63][64][65]. An indirect laser-induced fluorescence mode of detection by means of CdTe quantum dots has been demonstrated and therefore determination of small organic acids in food with detection limits in the range of tenths of mg/L was enabled [64].…”

Section: Enhancement Of Detectionmentioning

confidence: 99%

Capillary Electrophoresis in Nanotechnologies versus Nanotechnologies in Capillary Electrophoresis

Adam¹,

Vaculovičová²

2018

Novel Nanomaterials - Synthesis and Applications

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Nanomaterials are attracting an interest of many researches. All this attention is due the unique physical and chemical properties of nanomaterials differing significantly from the bulk materials mainly due to their size in range of nanometers. Capillary electrophoresis (CE) is a powerful, well-established analytical technique that provides numerous valuable benefits over other separation methods including high-performance liquid chromatography. The connection between CE and nanotechnology can be approached by two strategies: (i) CE analysis of nanomaterials and (ii) nanomaterials for CE improvement. The first perspective focuses on uses of CE as a method for characterization employed during nanomaterial production and modification as well as for monitoring their properties and interactions with other molecules. The second viewpoint deals with applications of nanomaterials for improving CE performance, mainly by enhancing efficiency of separation using nanomaterials as a stationary or pseudo-stationary phase and by enhancing detection sensitivity and/or selectivity in both optical and electrochemical detection. Moreover, applications of nanomaterials for sample preparation before CE analysis will be mentioned. This chapter aims at highlighting the symbiosis of CE and nanotechnology as a combination of modern, progressive field with well-known and reliable analytical method.

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“…In this study, we chose the CdSe quantum dots with a core shell structure, as these have good luminous performance and strong stability. Due to its unique optical and physical properties, QD is attracting increasing attention from scientific researchers, and has great potential for applications in biology and medicine to improve detection sensitivity and stability [7,8,9]. In particular, QD probe preparation for deployment in biological and medical immunity applications has been undergoing rapid development, especially for the detection of Vibrio parahaemolyticus [10] and C-reactive protein, and rapid diagnosis of infectious diseases such as African swine fever and Middle East respiratory syndrome [11][12].…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of brucellosis using a quantum dot-based immunochromatographic test strip

Rong

Wang

et al. 2020

PLoS Negl Trop Dis

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Novel diagnostic tools are a major challenge for brucellosis research, especially in developing countries. Herein, we established a handheld quantum dot (QD) immunochromatographic device for the fast detection of brucellosis antibodies in the field. Total bacterial protein extracted from Brucella 104M served as labelling and coating antigen. QD labelling and immunochromatography methods were used to optimise reaction conditions, labelling conditions, reaction temperature and storage temperature. QD test strips were employed to test brucellosis serum to determine their sensitivity, specificity and stability. Test strips were compared with Rose Bengal test, standard agglutination test and colloidal gold immunochromatographic assay. Labelled Brucella total protein displayed good specificity and no cross-reactivity. The concentration of labelled total bacterial protein was 3.9 mg/ml, the coating concentration was 2.0 mg/ ml, and the serum titre with the lowest detection sensitivity was 1:25. The optimal reaction temperature for the test strip was 25−30˚C. The test strip was stable after storage at room temperature and the repeatability was high, with a coefficient of variation of 4.0%. After testing 199 serum samples, the sensitivity of the QD test strip was 98.53%, the specificity was 93.57%, and the coincidence rate with the standard agglutination test was 96.98%. The developed QD immunochromatographic method can be used for rapid detection and preliminary screening of brucellosis in the field.

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Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

Cited by 14 publications

References 29 publications

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Capillary Electrophoresis in Nanotechnologies versus Nanotechnologies in Capillary Electrophoresis

Rapid detection of brucellosis using a quantum dot-based immunochromatographic test strip

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