Protegrin-1 inhibits porcine ovarian granulosa cell apoptosis from H2O2-induced oxidative stress via the PERK/eIF2α/CHOP signaling pathway in vitro

Li, Xuan; Lin, Yu‐Feng; Yao, Jiawei; Pan, Bo; Zhan, Xiaoshu; Chen, Zhisheng; Bai, Yinshan; Hui, Zhang; Wang, Bingyun; Li, Julang; Liu, Canying

doi:10.1016/j.theriogenology.2021.11.022

Cited by 9 publications

(8 citation statements)

References 46 publications

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“…The anti‐oxidative ability of kurarinone was also found in lipopolysaccharide‐treated RAW264.7 cells and collagen‐induced arthritis 14,15 . Granulosa cell apoptosis plays an essential role in follicular atresia, 42 which could be induced by H 2 O 2 stimulation 33,43 . In our study, we found that kurarinone mitigated H 2 O 2 ‐induced apoptosis of granulosa cells.…”

Section: Discussionsupporting

confidence: 71%

“…Apoptosis and oxidative stress of granulosa cells are main causes of follicular atresia, which lead to weakening fertility of women 33,34 . With the purpose of exploring new drug for therapy of granulosa cell damage in follicular atresia, here we firstly confirmed the anti‐oxidative and anti‐apoptotic roles of kurarinone in H 2 O 2 ‐treated KGN cells, and validated that it was linked to the IGF1/PI3K/Akt pathway.…”

Section: Discussionsupporting

confidence: 60%

“…Apoptosis and oxidative stress of granulosa cells are main causes of follicular atresia, which lead to weakening fertility of women. 33,34 With the purpose of exploring new drug for therapy of granulosa cell Kurarinone is a multifunctional natural compound, and our study first showed that it attenuated oxidative stress-induced cytotoxicity to granulosa cells, indicating the therapeutic potential in female follicular atresia. H 2 O 2 is an oxidizing agent that induces oxidative damage in multiple eukaryotic cells, such as liver cells, 35 retinal pigment epithelium cells, 36 astrocytes, 37 spermatogenic cells, 38 lung fibroblast cells, 39 and mesenchymal stem cells.…”

Section: Discussionmentioning

confidence: 76%

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Kurarinone attenuates hydrogen peroxide‐induced oxidative stress and apoptosis through activating the PI3K/Akt signaling by upregulating IGF1 expression in human ovarian granulosa cells

Yin

Yan

et al. 2022

Environmental Toxicology

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Dysregulated follicular development may lead to follicular atresia, and this is associated with oxidative stress in granulosa cells. Kurarinone is a natural compound possessing multiple activities, including antioxidative ability. However, the role of kurarinone in granulosa cell damage during follicular atresia remains unknown.Human ovarian granulosa KGN cells were treated with hydrogen peroxide (H 2 O 2 ) to induce cellular damage. Cytotoxicity was investigated by lactate dehydrogenase (LDH) release assay. Oxidative stress was evaluated by detection of reactive oxygen

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Section: Discussionsupporting

confidence: 71%

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 76%

See 1 more Smart Citation

Kurarinone attenuates hydrogen peroxide‐induced oxidative stress and apoptosis through activating the PI3K/Akt signaling by upregulating IGF1 expression in human ovarian granulosa cells

Yin

Yan

et al. 2022

Environmental Toxicology

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“…The antibodies included anti-SMAD4 (D120124, Sangon Biotech, 1:1000), anti-NR2C1 (A6675, ABclonal, 1:1000), anti- FoxO1 (D190665, Sangon Biotech, 1:1000), anti-GAPDH (TA802519, ORIGENE, 1:3000), and anti-β-Tubulin (10068-1-AP, Proteintech, 1:3000). β-Tubulin and GAPDH were measured as the internal control [ 12 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…For the H 2 O 2 treatment, the culture medium was first changed by DMEM/F12 without FBS for 6 h, and then H 2 O 2 (Sigma, shanghai, China) was added into the medium with the final concentration at 200 μM for 2 h [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

A Mutation in Endogenous saRNA miR-23a Influences Granulosa Cells Response to Oxidative Stress

Wang

Zeng

et al. 2022

Antioxidants

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Phenotypes are the result of the interaction between the gene and the environment, so the response of individuals with different genotypes to an environment is variable. Here, we reported that a mutation in miR-23a influences granulosa cells (GCs) response to oxidative stress, a common mechanism of environmental factors affecting female reproduction. We showed that nuclear miR-23a is a pro-apoptotic miRNA in porcine GCs through the activation of the transcription and function of NORHA, a long non-coding RNA (lncRNA) induces GC apoptosis and responses to oxidative stress. Mechanistically, miR-23a acts as an endogenous small activating RNA (saRNA) to alter histone modifications of the NORHA promoter through the direct binding to its core promoter. A C > T mutation was identified at −398 nt of the miR-23a core promoter, which created a novel binding site for the transcription factor SMAD4 and recruited the transcription repressor SMAD4 to inhibit miR-23a transcription and function in GCs. Notably, g.−398C > T mutation in the miR-23a promoter reduced GCs response to oxidative stress. In addition, g.−398C > T mutation was significantly associated with sow fertility traits. In short, our findings preliminarily revealed the genetic basis of individual differences in the response to oxidative stress from the perspective of a single mutation and identified miR-23a as a candidate gene for the environmental adaptation to oxidative stress.

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Isalo scorpion Cytotoxic peptide (IsCT) improved the physical barrier of the intestine on on-growing grass carp (Ctenopharyngodon idella)

Zhang¹,

Hu²,

Feng³

et al. 2023

Aquaculture

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Protegrin-1 inhibits porcine ovarian granulosa cell apoptosis from H2O2-induced oxidative stress via the PERK/eIF2α/CHOP signaling pathway in vitro

Cited by 9 publications

References 46 publications

Kurarinone attenuates hydrogen peroxide‐induced oxidative stress and apoptosis through activating the PI3K/Akt signaling by upregulating IGF1 expression in human ovarian granulosa cells

Kurarinone attenuates hydrogen peroxide‐induced oxidative stress and apoptosis through activating the PI3K/Akt signaling by upregulating IGF1 expression in human ovarian granulosa cells

A Mutation in Endogenous saRNA miR-23a Influences Granulosa Cells Response to Oxidative Stress

Isalo scorpion Cytotoxic peptide (IsCT) improved the physical barrier of the intestine on on-growing grass carp (Ctenopharyngodon idella)

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