Protective roles of estradiol against vascular oxidative stress in ovariectomized female rats exposed to normoxia or intermittent hypoxia

Ribon-Demars, Alexandra; Pialoux, Vincent; Boreau, Anaëlle; Marcouiller, François; Lariviere, Richard W.; Bairam, Aïda; Joseph, Vincent

doi:10.1111/apha.13159

Cited by 25 publications

(23 citation statements)

References 61 publications

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“…However, only the ERα agonist prevented the increase of arterial blood pressure during exposure to CIH. These results are in line with our recent study showing that E 2 treatment prevents oxidative stress in the brain cortex, adrenal medulla, and in the thoracic aorta of ovariectomized female rats exposed to a similar pattern of CIH.…”

Section: Discussionsupporting

confidence: 93%

“…In contradistinction with these previous findings, current results show that under exposure to CIH, PPT and DPN do not increase the activity of complex IV, but rather prevent the reduction of complex I activity. We have recently reported that in the thoracic aorta of ovariectomized female rats the effects of E 2 on pro‐ and antioxidant enzyme activities are different under normoxic and CIH conditions . Therefore, it is possible that the discrepancy observed between the present results and those reported by Irwin et al may be accounted for by the CIH exposures in our study.…”

Section: Discussioncontrasting

confidence: 87%

“…We have recently reported that in the thoracic aorta of ovariectomized female rats the effects of E 2 on pro-and antioxidant enzyme activities are different under normoxic and CIH conditions. 38 Therefore, it is possible that the discrepancy observed between the present results and those reported by Irwin et al 21 may be accounted for by the CIH exposures in our study.…”

Section: Ppt and Dpn Prevent The Mitochondrial Dysfunction Induced contrasting

confidence: 78%

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Roles of oestradiol receptor alpha and beta against hypertension and brain mitochondrial dysfunction under intermittent hypoxia in female rats

et al. 2019

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Aim Chronic intermittent hypoxia (CIH) induces systemic (hypertension) and central alterations (mitochondrial dysfunction underlying cognitive deficits). We hypothesized that agonists of oestradiol receptors (ER) α and β prevent CIH‐induced hypertension and brain mitochondrial dysfunction. Methods Ovariectomized female rats were implanted with osmotic pumps delivering vehicle (Veh), the ERα agonist propylpyraoletriol (PPT — 30 μg/kg/day) or the ERβ agonist diarylpropionitril (DPN — 100 μg/kg/day). Animals were exposed to CIH (21%‐10% FIO2 — 10 cycles/hour – 8 hours/day – 7 days) or normoxia. Arterial blood pressure was measured after CIH or normoxia exposures. Mitochondrial respiration and H2O2 production were measured in brain cortex with high‐resolution respirometry, as well as activity of complex I and IV of the electron transport chain, citrate synthase, pyruvate, and lactate dehydrogenase (PDH and LDH). Results Propylpyraoletriol but not DPN prevented the rise of arterial pressure induced by CIH. CIH exposures decreased O2 consumption, complex I activity, and increased H2O2 production. CIH had no effect on citrate synthase activity, but decreased PDH activity and increased LDH activity indicating higher anaerobic glycolysis. Propylpyraoletriol and DPN treatments prevented all these alterations. Conclusions We conclude that in OVX female rats, the ERα agonist prevents from CIH‐induced hypertension while both ERα and ERβ agonists prevent the brain mitochondrial dysfunction and metabolic switch induced by CIH. These findings may have implications for menopausal women suffering of sleep apnoea regarding hormonal therapy.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 87%

Section: Ppt and Dpn Prevent The Mitochondrial Dysfunction Induced contrasting

confidence: 78%

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Roles of oestradiol receptor alpha and beta against hypertension and brain mitochondrial dysfunction under intermittent hypoxia in female rats

et al. 2019

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“…On the other hand, Prog does not prevent the CIH‐induced increase in arterial blood pressure. These results complement our previous dataset showing the role of E 2 (Laouafa et al., 2017; Ribon‐Demars et al., 2018) and selective agonists of E 2 receptors (Laouafa et al., 2019) on oxidative stress, respiratory irregularities and arterial blood pressure in a similar experimental design.…”

Section: Discussionsupporting

confidence: 90%

“…Oxidative stress in CIH‐exposed animals and sleep apnoea patients is thought to have several harmful consequences, including high activity of the sympathetic nervous system (driven by peripheral chemoreceptors), arterial hypertension, cardiovascular diseases and neuronal damage (Almendros et al., 2014; Lavie, 2015; Prabhakar et al., 2015). In line with a rich literature showing antioxidant properties of E 2 , we have demonstrated that in female rats exposed to CIH, E 2 supplementation prevented the elevation of arterial blood pressure and oxidative stress damage in the brain cortex, brainstem (Laouafa et al., 2017) and thoracic aorta (Ribon‐Demars et al., 2018). Furthermore selective agonists of the E 2 α and β receptors (ERα and ERβ) prevented the elevation of mitochondrial ROS production during CIH exposure, but only the ERα agonist prevented the increase of arterial blood pressure (Laouafa et al., 2019).…”

Section: Introductionsupporting

confidence: 84%

Progesterone decreases apnoea and reduces oxidative stress induced by chronic intermittent hypoxia in ovariectomized female rats

Joseph

Laouafa

Marcouiller

et al. 2020

Experimental Physiology

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We tested the hypothesis that progesterone (Prog) reduces the effect of chronic intermittent hypoxia (CIH) on arterial blood pressure, respiratory chemoreflexes and oxidative stress in the central nervous system. Ovariectomized female rats were implanted with osmotic pumps delivering vehicle (Veh) or Prog (4 mg kg −1 day −1 ). Two weeks following the surgery, rats were exposed to room air (Air) or CIH (7 days, 10% O 2 , 10 cycles h −1 , 8 h day −1 ). We studied three groups: Veh-Air, Veh-CIH and Prog-CIH. After the CIH exposures, we measured the mean arterial pressure (MAP; tail cuff) and assessed the frequency of apnoeas at rest and ventilatory responses to hypoxia and hypercapnia (whole body plethysmography). The activities of the pro-oxidant enzyme NADPH oxidase (NOX) and antioxidant enzymes superoxide dismutase (SOD; in mitochondrial and cytosolic fractions) and glutathione peroxidase (GPx), as well as the concentration of malondialdehyde (MDA), a marker of lipid peroxidation, were measured in brain cortex and brainstem samples. CIH exposure increased the MAP, the frequency of apnoeas, and the respiratory frequency response to hypoxia and hypercapnia. Prog did not prevent the CIHinduced elevation in MAP, but it reduced the CIH-induced frequency of apnoeas and increased hypoxic and hypercapnic ventilatory responses. In the brain cortex, CIH increased NOX activity, and decreased the cytosolic and mitochondrial SOD activities. These effects were prevented by Prog. NOX activity was increased by CIH in the brainstem, and this was also blocked by Prog. The study draws focus to the links between ovarian hormones and the consequences of sleep apnoea in women. K E Y W O R D Sintermittent hypoxia, oxidative stress, progesterone

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Role of Oxidative Stress and Interrelated Cellular Offences in Sex Modulation of Cardiorenal Sequels of Sepsis

El-Lakany,

Wedn,

El-Mas

2024

Oxidative Stress in Applied Basic Research and Clinical Practice

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Protective roles of estradiol against vascular oxidative stress in ovariectomized female rats exposed to normoxia or intermittent hypoxia

Abstract: E -based therapy could help prevent the vascular consequences of CIH in apneic women.

Cited by 25 publications

References 61 publications

Roles of oestradiol receptor alpha and beta against hypertension and brain mitochondrial dysfunction under intermittent hypoxia in female rats

Roles of oestradiol receptor alpha and beta against hypertension and brain mitochondrial dysfunction under intermittent hypoxia in female rats

Progesterone decreases apnoea and reduces oxidative stress induced by chronic intermittent hypoxia in ovariectomized female rats

Role of Oxidative Stress and Interrelated Cellular Offences in Sex Modulation of Cardiorenal Sequels of Sepsis

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