Protective Roles of Cytosolic and Plastidal Proteasomes on Abiotic Stress and Pathogen Invasion

Ali, Md. Sarafat; Baek, Kwang-Hyun

doi:10.3390/plants9070832

Cited by 23 publications

(23 citation statements)

References 157 publications

(198 reference statements)

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“…The genomic region also carries gene encoding for ATPdependent Clp protease ATP-binding subunit. Clp protease degrades damaged or non-native proteins in mitochondria and chloroplasts whose amount increases during abiotic and biotic stress conditions (Ali and Baek, 2020).…”

Section: Discussionmentioning

confidence: 99%

Characterization and Mapping of Spot Blotch in Triticum durum–Aegilops speltoides Introgression Lines Using SNP Markers

Kaur

Dhillon

et al. 2021

Front. Plant Sci.

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Spot blotch (SB) of wheat is emerging as a major threat to successful wheat production in warm and humid areas of the world. SB, also called leaf blight, is caused by Bipolaris sorokiniana, and is responsible for high yield losses in Eastern Gangetic Plains Zone in India. More recently, SB is extending gradually toward cooler, traditional wheat-growing North-Western part of the country which is a major contributor to the national cereal basket. Deployment of resistant cultivars is considered as the most economical and ecologically sound measure to avoid losses due to this disease. In the present study, 89 backcross introgression lines (DSBILs) derived from Triticum durum (cv. PDW274-susceptible) × Aegilops speltoides (resistant) were evaluated against SB for four consecutive years, 2016–2020. Phenotypic evaluation of these lines showed a continuous variation in disease severity indicating that the resistance to SB is certainly quantitative in nature. Phenotypic data of DSBILs were further used for mapping QTLs using SNPs obtained by genotyping by sequencing. To identify QTLs stable across the environments, Best Linear Unbiased Estimates (BLUEs) and Predictions (BLUPs) were used for mapping QTLs based on stepwise regression-based Likelihood Ratio Test (RSTEP-LRT) for additive effect of markers and single marker analysis (SMA). Five QTLs, Q.Sb.pau-2A, Q.Sb.pau-2B, Q.Sb.pau-3B, Q.Sb.pau-5B, and Q.Sb.pau-6A, linked to SB resistance were mapped across chromosomes 2A, 2B, 3B, 5B, and 6A. Genes found adjacent to the SNP markers linked to these QTLs were literature mined to identify possible candidate genes by studying their role in plant pathogenesis. Further, highly resistant DSBIL (DSBIL-13) was selected to cross with a susceptible hexaploidy cultivar (HD3086) generating BC2F1 population. The QTL Q.Sb.pau-5B, linked to SNP S5B_703858864, was validated on this BC2F1 population and thus, may prove to be a potential diagnostic marker for SB resistance.

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Section: Discussionmentioning

confidence: 99%

Characterization and Mapping of Spot Blotch in Triticum durum–Aegilops speltoides Introgression Lines Using SNP Markers

Kaur

Dhillon

et al. 2021

Front. Plant Sci.

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“…Moreover, plant ClpB proteins can be cytosolic ( Agarwal et al, 2002 ; Singh and Grover, 2010 ), whereas other Clp subtypes are generally localized to the chloroplasts or the mitochondria and contain a ClpP interaction motif ( Nishimura and van Wijk, 2015 ). In these organelles, the ClpP complexes mainly perform a housekeeping function similar to that of the nuclear and cytoplasmic 26S proteasomes, i.e., the degradation of incorrectly neosynthesized proteins ( Ali and Baek, 2020 ).…”

Section: Smxl Are Plant-specific Atypical Clp-atpasesmentioning

confidence: 99%

Masks Start to Drop: Suppressor of MAX2 1-Like Proteins Reveal Their Many Faces

et al. 2022

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Although the main players of the strigolactone (SL) signaling pathway have been characterized genetically, how they regulate plant development is still poorly understood. Of central importance are the SUPPRESSOR OF MAX2 1-LIKE (SMXL) proteins that belong to a family of eight members in Arabidopsis thaliana, of which one subclade is involved in SL signaling and another one in the pathway of the chemically related karrikins. Through proteasomal degradation of these SMXLs, triggered by either DWARF14 (D14) or KARRIKIN INSENSITIVE2 (KAI2), several physiological processes are controlled, such as, among others, shoot and root architecture, seed germination, and seedling photomorphogenesis. Yet another clade has been shown to be involved in vascular development, independently of the D14 and KAI2 actions and not relying on proteasomal degradation. Despite their role in several aspects of plant development, the exact molecular mechanisms by which SMXLs regulate them are not completely unraveled. To fill the major knowledge gap in understanding D14 and KAI2 signaling, SMXLs are intensively studied, making it challenging to combine all the insights into a coherent characterization of these important proteins. To this end, this review provides an in-depth exploration of the recent data regarding their physiological function, evolution, structure, and molecular mechanism. In addition, we propose a selection of future perspectives, focusing on the apparent localization of SMXLs in subnuclear speckles, as observed in transient expression assays, which we couple to recent advances in the field of biomolecular condensates and liquid–liquid phase separation.

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“…Peptidases are responsible for the degradation of proteins. Under heat shock conditions, many proteins may be misfolded, damaged, and mislocalized (Ali and Baek, 2020). We also report the presence of 3 times more number of peptidases in hot spring species compared to non-hot spring PCC 7120 species (UU774: 78, PCC 9339: 89, NIES-3754: 80, and PCC 7120: 30).…”

Section: Heat Resistance Possibly Attributed To the Presence And Cons...mentioning

confidence: 58%

Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement

et al. 2022

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The present study focuses on the stress response of a filamentous, AT-rich, heterocystous cyanobacterium Mastigocladus laminosus UU774, isolated from a hot spring, Taptapani, located in the eastern part of India. The genome of UU774 contains an indispensable fragment, scaffold_38, of unknown origin that is implicated during severe nitrogen and nutrition stress. Prolonged exposure to nitrogen compounds during starvation has profound adverse effects on UU774, leading to loss of mobility, loss of ability to fight pathogens, reduced cell division, decreased nitrogen-fixing ability, reduced ability to form biofilms, reduced photosynthetic and light-sensing ability, and reduced production of secreted effectors and chromosomal toxin genes, among others. Among genes showing extreme downregulation when grown in a medium supplemented with nitrogen with the fold change > 5 are transcriptional regulator gene WalR, carbonic anhydrases, RNA Polymerase Sigma F factor, fimbrial protein, and twitching mobility protein. The reduced expression of key enzymes involved in the uptake of phosphate and enzymes protecting oxygen-sensitive nitrogenases is significant during the presence of nitrogen. UU774 is presumed to withstand heat by overexpressing peptidases that may be degrading abnormally folded proteins produced during heat. The absence of a key gene responsible for heterocyst pattern formation, patS, and an aberrant hetN without a functional motif probably lead to the formation of a chaotic heterocyst pattern in UU774. We suggest that UU774 has diverged from Fischerella sp. PCC 9339, another hot spring species isolated in the United States.

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Protective Roles of Cytosolic and Plastidal Proteasomes on Abiotic Stress and Pathogen Invasion

Cited by 23 publications

References 157 publications

Characterization and Mapping of Spot Blotch in Triticum durum–Aegilops speltoides Introgression Lines Using SNP Markers

Characterization and Mapping of Spot Blotch in Triticum durum–Aegilops speltoides Introgression Lines Using SNP Markers

Masks Start to Drop: Suppressor of MAX2 1-Like Proteins Reveal Their Many Faces

Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement

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