Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro

Abstract

Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the eff ects of DDVP (1, 10, 100 μM) or DDVP + vitamin C (VC; 10 μM) or vitamin E (VE; 30 μM), on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes were examined in vitro. There were no statistical diff erences between all groups for 1 μM concentration of DDVP. Treatment with DDVP alone produced an increase in the level of MDA and decreased activities of antioxidant enzymes (P < 0.05). Groups treated with vitamins and DDVP showed protective eff ects of vitamins against DDVP-induced changes in antioxidant enzyme activity and lipid peroxidation (LPO) (10 μM). At 100 μM concentration of DDVP vitamins had no eff ect on DDVP-induced toxicity. The results show that administration of DDVP resulted in the induction of erythrocyte LPO and alterations in antioxidant enzyme activities, suggesting that reactive oxygen species (ROS) may be involved in the toxic eff ects of DDVP. Also the data show that the plasma level of VC and VE may ameliorate OP-induced oxidative stress by decreasing LPO in erythrocytes at certain doses of OP pesicides.

Toxicity Effect of Ultraviolet (UV) Radiations on the Human Germ Cell and the Protective Role of Vitamin E

“…Several studies have also shown that α-tocopherol inhibits free radical formation and may effectively minimize lipid peroxidation in biological systems [36]. Antioxidant addition to the diet has been shown to enhance free radical scavenging role and decrease oxidative stress [38]. Our obtained data support these statements.…”

Effect of enrofloxacin and chlorpyrifos on the levels of vitamins A and E in Wistar rats

“…However, most of the research concerned the protective effects of these vitamins, which minimized effectively lipid peroxidation in biological systems, in pesticide intoxication (Eroglu et al, 2013;Ojha and Srivastava, 2012) A decrease in the vitamin A level has also been observed in studies on intoxication with other types of pesticides. The content of retinol in the liver was nearly 50% lower in young common kestrels exposed to PBDEs (polybrominated diphenyl ethers) in comparison to the control group of birds (Fernie et al, 2005).…”

The Effect of LDL-Apheresis and Rheohaemapheresis Treatment on Vitamin E

“…Exposure to fluoride induces increased lipid peroxidation measured as malondialdehyde by altering the activity of antioxidant enzymes: SOD, CAT, and GPx (49-51). The major function of GPx is to reduce soluble hydrogen peroxide and alkyl peroxides (52). GPx uses glutathione as the hydrogen donor, and produces GSSG (glutathione disulfide) and water (53).…”