Protective role of vitamin E preconditioning of human dermal fibroblasts against thermal stress in vitro

Butt, Hira; Mehmood, Azra; Muhammad, Ali; Tasneem, Saba; Anjum, Muhammad Sohail; Tarar, Moazzam Nazeer; Khan, Shaheen N.; Riazuddin, Sheikh

doi:10.1016/j.lfs.2017.07.002

Cited by 30 publications

(17 citation statements)

References 45 publications

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“…NAC and Vitamin E are known antioxidants, which have been shown to be effective in attenuating cellular senescence 43 – 47 . Dose concentration curves were generated to determine the optimal doses of NAC and Vitamin E, which were somewhat effective in attenuating senescence in our H 2 O 2 model (Fig.…”

Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: The Na/K-ATPase Oxidant Amplification Loop Regulates Aging

Sodhi

Nichols

Mallick

et al. 2018

Sci Rep

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As aging involves oxidant injury, we examined the role of the recently described Na/K-ATPase oxidant amplification loop (NKAL). First, C57Bl6 old mice were given a western diet to stimulate oxidant injury or pNaKtide to antagonize the NKAL. The western diet accelerated functional and morphological evidence for aging whereas pNaKtide attenuated these changes. Next, human dermal fibroblasts (HDFs) were exposed to different types of oxidant stress in vitro each of which increased expression of senescence markers, cell-injury, and apoptosis as well as stimulated the NKAL. Further stimulation of the NKAL with ouabain augmented cellular senescence whereas treatment with pNaKtide attenuated it. Although N-Acetyl Cysteine and Vitamin E also ameliorated overall oxidant stress to a similar degree as pNaKtide, the pNaKtide produced protection against senescence that was substantially greater than that seen with either antioxidant. In particular, pNaKtide appeared to specifically ameliorate nuclear oxidant stress to a greater degree. These data demonstrate that the NKAL is intimately involved in the aging process and may serve as a target for anti-aging interventions.

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Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: The Na/K-ATPase Oxidant Amplification Loop Regulates Aging

Sodhi

Nichols

Mallick

et al. 2018

Sci Rep

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“…This shows the synergistic mechanism of the combination of the EGF and TRF in the burn wounds healing processes of this study. These results are in agreement with a previously published study demonstrating the synergistic mechanism of vitamin E and EGF in protecting dermal fibroblasts in response to heat stress [38]. Collectively, the present findings provide clear evidence to suggest the potential benefits of the phyto-nutrients, like tocotrienol from oil palm TRF, to be useful as remedial agents in post-burn wound healing.…”

Section: Discussionsupporting

confidence: 93%

Healing Properties of Epidermal Growth Factor and Tocotrienol-Rich Fraction in Deep Partial-Thickness Experimental Burn Wounds

et al. 2020

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Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided into six groups of six each and were: untreated control, treated with Silverdin® cream, base cream, base cream with c% EGF, base cream with 3% TRF or base cream with c% EGF and 3% TRF, respectively. Creams were applied once daily for 21 consecutive days. Six animals from each group were sacrificed using anaesthetic overdose on the third, seventh, 11th, 14th and 21st day post-burn. Skin tissues with the wound to be examined were excised for macroscopic and microscopic evaluation and biochemical analyses. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. However, no effects on the number of adipose cells in the healing process were recorded. In addition, lipid peroxidation and nitrite production were found to be reduced post-burn, reducing oxidative stress. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters.

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“…Vitamin E acts as a peroxyl radical scavenger; it reacts with ROS and forms tocopheryl radicals [ 29 , 58 , 59 ]. It also has an effect on the expression of selected genes (e.g., connective tissue growth factor (CTGF) and others), which are important in wound repair and regeneration [ 38 , 39 , 60 ]. In a very recent study by Mancio et al, vitamin E improved skeletal muscle injury and promoted membrane repair in mouse models [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

“…Vitamin E is also known to be beneficial for postmenopausal women as it helps to relieve symptoms like night sweats or hot flashes [ 26 , 37 ]. In some cases, vitamin E remarkably improves the release of paracrine factors like epidermal growth factor (EGF) or vascular endothelial growth factor (VEGF) [ 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

Ciebiera

Szymańska-Majchrzak

Sentkowska

et al. 2018

BioMed Research International

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Uterine fibroids (UFs) are benign tumors of the reproductive tract, arising from smooth muscle cells of the uterus. Steroid hormones, estrogen, and progesterone are considered to be the most important links in the pathophysiology of UFs. Alpha-tocopherol (AT) is the most active form of vitamin E. What is important as far as UFs are concerned is that ATs contain structural determinants, which makes them possible ligands for estrogen receptors (ERs). We present a retrospective cohort study performed in a university teaching hospital. We included a total of 162 patients divided into 2 groups: with UFs and controls. The effects of age, body mass index (BMI), positive medical history, parity, and AT serum concentrations on the risk for the development of UFs were investigated. Mean AT serum concentrations were 11.66 ± 4.97 μg/ml and 7.83 ± 3.13 μg/ml (medians 10.56 μg/ml and 7.42 μg/ml) in patients with UFs confirmed on ultrasound and controls, respectively. The presented difference was statistically significant. Higher BMI, positive family history, and low parity were found to be major risk factors for UFs. In our study, we confirmed that elevated serum AT concentration might be an important risk factor for UFs in Caucasian women. Further research in this area is necessary.

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Protective role of vitamin E preconditioning of human dermal fibroblasts against thermal stress in vitro

Cited by 30 publications

References 45 publications

RETRACTED ARTICLE: The Na/K-ATPase Oxidant Amplification Loop Regulates Aging

RETRACTED ARTICLE: The Na/K-ATPase Oxidant Amplification Loop Regulates Aging

Healing Properties of Epidermal Growth Factor and Tocotrienol-Rich Fraction in Deep Partial-Thickness Experimental Burn Wounds

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

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