Protective role of Royal Jelly (honeybee) on genotoxicity and lipid peroxidation, induced by petroleum wastewater, inAllium cepaL. root tips

Abstract

In the present study, the protective effect of Royal Jelly (RJ) on genotoxicity and lipid peroxidation, induced by petroleum wastewater, in Allium cepa L. root-tip cells was investigated. For this purpose, we used the malondialdehyde (MDA) level, mitotic index (MI), frequency of micronucleus (MN) and chromosomal aberrations (CAs) as indicators of genotoxicity and lipid peroxidation, and correlated these data with statistical parameters. In additional to the genotoxic analysis, we examined changes in the root anatomy of A. cepa seeds treated with the wastewater. Heavy metal concentrations in the wastewater were measured by atomic absorption spectrophotometry. The seeds were divided into six groups as control, wastewater and RJ treatment groups. They were treated with the wastewater alone, RJ alone (25 and 50 microm doses) and RJ + wastewater for 10 consecutive days. As a result, the mean concentrations of heavy metals in the wastewater were observed to be in the order: Pb > Fe > Al > Ni > Cu > Zn > Cr > Cd. The results showed that there was a significant alteration in MI and in the frequency of MN and CAs in the seeds exposed to the wastewater when compared with the controls. The wastewater exposure resulted in a significant increase in CAs and MN formation (P < 0.05). The wastewater also caused a decrease in MI (P < 0.05). Additionally, there was a significant increase in the MDA levels of the roots exposed to the wastewater (P < 0.05). Heavy metals in the petroleum wastewater significantly increased the MDA production, indicating lipid peroxidation. Moreover, light micrographs showed anatomical damages such as an accumulation of chemical compounds in cortex parenchyma, cell death, an unusual form of cell nucleus and unclear vascular tissue. However, the RJ treatment caused amelioration in the indices of lipid peroxidation and MI, and in the frequency of CAs and MN, when compared with the group treated with petroleum wastewater alone (P < 0.05). Also, the RJ application caused the recuperation of anatomical structural damages induced by the petroleum wastewater. Each dose of RJ provided protection against the wastewater toxicity, and the strongest protective effect was observed at dose of 50 microm. In vivo results showed that RJ is a potential protector against toxicity induced by petroleum wastewater, and its protective role is dose-dependent.

Royal jelly enhances migration of human dermal fibroblasts and alters the levels of cholesterol and sphinganine in anin vitrowound healing model

“…In contrast to our results, previous reports found that in cultured skin substitutes grown in vitro the levels of cholesterol and free fatty acids were increased during the healing process with grafting [10,26]. An increase in the levels of lipids in HDFn, in particular sphingolipids such as ceramides and glucosylceramides, has been noted to occur in the wound healing process [27,28]. Also, increases in the levels of glucosylceramides and ceramides were highly distinct [26,29].…”

The Effects of <i>Aloe vera</i> L. Leaf Extract on Some Physiological and Cytogenetical Parameters in <i>Allium cepa</i> L. Seeds Germinated under Salt Stress

“…as reported by many researchers. MN formation results from different types of chromosomal aberrations such as their inhibition or delay in the formation of the chromosome-spindle complex (İnceer et al 2003, Türkmen et al 2009, Arya and Mukherjee 2014.…”

“…Healthy and approximately equal-sized A. cepa seeds were selected. The seeds were sterilized with 2.5% sodium hypochloride solution for 10 min and washed for 24 h in ultra-distilled water (Türkmen et al 2009). Twenty seeds from each treatment group were placed into plastic containers.…”

The protective role of honey against cytotoxicity of cadmium chloride in mice

“…Honey sugar displayed complex behavior toward the enhanced or inhibited mutagenic activity in a model by cooked food systems (Skog, 1993). Royal jelly (RJ) had shown highly efficient antioxidant where it scavenges the produced free radicals (Cemek et al, 2010;ElNekeety et al, 2007;Türkmen, 2009). El-Monem (2011) also revealed that RJ caused a significant recovery in antioxidant status.…”