Protective role of nitric oxide in indomethacin-induced gastric ulceration by a mechanism independent of gastric acid secretion

Khattab, Mahmoud M.; Gad, Mohamed Z.; Abdallah, Dalaal M.

doi:10.1006/phrs.2001.0801

Cited by 70 publications

(62 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study showed down regulation of treatment with indomethacin to gastric tissue eNOS gene resulting in a decrement in gastric level of NO leading to a decrease in mucosal synthesis and mucosal barrier content which confirmed by biochemical and histopathological analysis. Similarly, previous studies showed a decrease in NO production by indomethacin [34][35][36][37]. Meanwhile pretreatment with vanillin increased eNOS gene expression as well as gastric level of NO leading to increasing mucus synthesis plus restoration of the depleted gastric mucus levels.…”

Section: Discussionsupporting

confidence: 65%

“…Mainly, cytoprotective endothelial (eNOS) and cytotoxic inducible (iNOS) [13]. Nitric oxide from cNOS improves the mucosal blood flow, protects the integrity of epithelial tissue and inhibits activation, adhesion and migration of leucocytes in the inflammatory [34] resulting in increasing mucus synthesis and accelerating ulcer healing [12]. This study showed down regulation of treatment with indomethacin to gastric tissue eNOS gene resulting in a decrement in gastric level of NO leading to a decrease in mucosal synthesis and mucosal barrier content which confirmed by biochemical and histopathological analysis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

Ma¹,

Salahuddin²

2017

Clin Exp Pharmacol

View full text Add to dashboard Cite

Indomethacin provokes aggressive ulcerogenic adverse effects. Natural products with fewer side effects are therefore highly requested to attenuate its gastric ulcer effect. Vanillin is a natural compound widely used as flavoring agent which has antioxidant activity. Therefore, the aim of this study was to investigate its gastroprotective effect against indomethacin induced gastric injury. Rats were divided into four groups; first group served as control, group 2: Treated with indomethacin (25 mg/kg, po.), group 3: Pre-treated with ranitidine (reference drug) (50 mg/kg, po., 5 days) before indomethacin and group 4: Pretreated with vanillin (100 mg/kg, po., 5 days). Pre-treatment with vanillin reduced ulcer index, gastric juice volume, free, total acidity and histopathological changes induced by indomethacin. Although it reduced gastric oxidative stress, it elevated enzymatic antioxidant activity and gastric nitric oxide content. Moreover, it reduced gastric NFκB protein expression and activity as well as inhibition in levels of proinflammatory cytokines, Myeloperoxidase (MPO) and caspase 3 activities. It down regulated gene expression of TNF-α, Cytokine-Induced Neutrophil Chemo Attractant (CINC-2α) and caspase-9 while lacking effect on mucosal prostaglandin E2 (PGE2) level. Collectively, vanillin displayed gastroprotective effects in indomethacin induced gastric ulcer by anti-secretory action and cytoprotective effect via antioxidant and anti-inflammatory activities.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

Ma¹,

Salahuddin²

2017

Clin Exp Pharmacol

View full text Add to dashboard Cite

show abstract

“…22 The kidney tissue of each rat was scrubbed off, weighed, and its nitric oxide content was determined as nitrite by diazotization with sulfanilic acid at acidic pH and subsequent coupling with N-1-naphthyl-ethylene diamine to give a colored product that was measured colorimetrically at 548 nm.…”

Section: Nephritic No Levelmentioning

confidence: 99%

L-Citrulline Protects Against Glycerol-Induced Acute Renal Failure in Rats

Liu

Gou

et al. 2013

Renal Failure

View full text Add to dashboard Cite

There is an increasing evidence that oxidative stress plays an important role in the pathogenesis of rhabdomyolysisinduced acute renal failure (ARF). In this study, protective effects of L-citrulline on glycerol-induced ARF in rats were investigated. Six groups of rats were employed in this study: group 1 served as a control; group 2 was only given glycerol (50%, 10 mL/kg, i.m.); group 3 was given glycerol plus dexamethasone (0.1 mg/kg, i.g.) as positive reference drug, starting at the same time as the glycerol injections; the last three groups were given glycerol plus L-citrulline (300, 600, and 900 mg/kg, i.g.) respectively, starting at the same time as the glycerol injections. The injections of glycerol were only once, and after glycerol injections the i.g. administrations of dexamethasone and L-citrulline were repeated every 24 h for 7 days. After 7 days of glycerol injections, the blood samples and kidney tissues were harvested for future biochemical and pathology analyses. The levels of creatinine (Cr) and urea nitrogen (BUN) in plasma, the content of malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), the activity of total nitric oxide synthase (TNOS), inducible nitric oxide synthase (iNOS), endothelial NO synthase (eNOS), and superoxide dismutase (SOD) were evaluated in kidney tissues. Consequently, administrations of L-citrulline improved an impaired intrarenal oxygenation and kidney function compared with the glycerol group, and prevented the renal oxidative stress damage as well as severe functional and morphological renal deterioration. Therefore, L-citrulline might have potential application in the amelioration of glycerol-induced ARF.

show abstract

“…Many experimental studies revealed that L-arginine has beneficial effect on the healing of gastric ulcer in rats and referred it to the release of nitric oxide which plays an important role in gastric mucosal defense mechanisms though maintaining mucosal integrity, controlling gastric motility and secretion, enhancing mucosal blood flow and wound healing (Elliot et al, 1995, Khattab et al, 2001, Nishio et al, 2006, Ajiboye et al, 2014and Usman et al, 2014. NO has the ability to activate COX-1 and COX-2 enzymes responsible for gastric defense via the action of prostaglandins E and F2α.…”

Section: Discussionmentioning

confidence: 99%

“…Nitric oxide (NO) is produced from L-arginine by endothelial NO synthase and plays an important role in regulating endothelium-dependent vasodilatation, preventing the adhesion of blood cells and platelets along the lining endothelial cells and has scavenging effects against oxygen radical species (Morita et al, 2014). Many researchers demonstrate the benefits of L-arginine in alleviation of gastric ulcer induced by nonsteroidal anti-inflammatory drugs as indomethacin and ibuprofen (Khattab et al, 2001, Sanchez-Fidalgo et al 2005 and Ajiboye et al 2014). The aim of the present work was to study the histological and immunohistochemical effect of leflunomide on the fundic mucosa of adult male albino rat and the possible ameliorative role of L-arginine.…”

Section: Introductionmentioning

confidence: 99%

Histological study of the effect of leflunomide on fundic mucosa of Adult male albino rat and the possible alleviating effect of L-arginine

Husseiny

Mihi

et al. 2017

Journal of Medical Histology

View full text Add to dashboard Cite

Leflunomide is a disease-modifying anti-rheumatic drug used in the treatment of rheumatoid arthritis due to its anti-inflammatory and immunosuppressive effects. This study aimed to investigate the histological effect of leflunomide on the fundic gastric mucosa of adult male albino rat and the possible ameliorative role of the non-essential amino acid l-arginine. Fifty adult male albino rats were divided randomly into four main groups, group I (control group), group II treated with leflunomide, group III received the L-arginine and group IV received the leflunomide and the l-arginine concomitantly. The four groups were subdivided into two subgroups; the first received the drugs for two weeks and the second received the drugs for four weeks. At the end of the experiment, all rats were sacrificed; the fundic mucosa was dissected out and processed for light and electron microscopic study. Paraffin sections were stained with hematoxylin and eosin, periodic acid Schiff (PAS), Mallory trichrome and immunohistochemically for PCNA antibody. Leflunomide treated (group II) showed sloughing and desquamation of epithelial cells, superficial ulceration, and inflammatory cellular infiltration. Electron microscopic examination revealed absence of apical microvilli and coalescence of secretory granules. L-arginine ameliorated to some extent the histological changes occurred by leflunomide. It can be concluded that leflunomide exerts some histological changes on fundic gastric mucosa and co-administration of l-arginine had ameliorating effects on the histological changes.

show abstract

Protective role of nitric oxide in indomethacin-induced gastric ulceration by a mechanism independent of gastric acid secretion

Cited by 70 publications

References 22 publications

Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

L-Citrulline Protects Against Glycerol-Induced Acute Renal Failure in Rats

Histological study of the effect of leflunomide on fundic mucosa of Adult male albino rat and the possible alleviating effect of L-arginine

Contact Info

Product

Resources

About