Protective role of intratympanic nigella sativa oil against gentamicin induced hearing loss

Edızer, Deniz Tuna; Yiğit, Özgür; Çınar, Zehra; Gül, Mehmet; Kara, Eyyüp; Yiğitcan, Birgül; Hayır, Duygu; Ataş, Ahmet

doi:10.1016/j.ijporl.2017.03.034

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…At the start point, there was non-significant variation between groups with normal ABR in all animals, while at the end point there was significantly increased ABR threshold compared to the start one. Our results in group 2 compared to group 1 showed absence of ABR response in 75% of animals indicating sever hearing loss, while others showed significant increase in wave III latency, wave V latency and significant ABR threshold increase which coincides with many studies [5,22,29] . The ABR results are matched with histopathology in light and SEM where there are variable degrees of HCs death, pathological changes in stria vascularis and degeneration of spiral ganglion which are in line with many studies [22,30,31] .…”

Section: Discussionsupporting

confidence: 90%

“…It affects mainly the basal hair cells of organ of corti. The incidence of aminoglycoside hearing loss is up to 25% [4,5] .There are multiple mechanisms of aminoglycosides ototoxicity; mutation in mitochondrial DNA [6] , oxidative stress [7] , phosphoinositide lipids involvement [8] , and autophagy has recently been proposed to play a crucial role [9] . There are two pathways for autophagy induction: rapamycin (mTOR)-dependent mammalian target, and mTOR-independent pathway [10] .…”

Section: Introductionmentioning

confidence: 99%

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Targeting Autophagy Induction as A possible Protective Mechanism by Verapamil Compared to Rapamycin (Sirolimus) Against Gentamicin -Induced Ototoxicity in Guinea Pigs

Eman Elzayat,

Amany A. Abdin,

Sabiha Hedya

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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Background: Ototoxicity is a harmful feature of the cochlea or auditory nerve and sometimes vestibular apparatus. Gentamicin is known to cause irreversible bilateral ototoxicity. Autophagy induction has been proposed as a target for prevention of gentamicin ototoxicity. Aim of the work: to investigate the possible protective effect of autophagy induction by verapamil compared to rapamycin. Methods: This experiment was conducted on 32 male guinea pigs. At the beginning each animal's hearing status was assessed using auditory brainstem response (ABR) audiometry. Then, they were divided into 4 equal groups: Group 1: control group, Group 2: untreated gentamicin-induced ototoxicity group. Group 3: gentamicin-induced ototoxicity treated concomitantly with rapamycin. Group 4: gentamicin-induced ototoxicity treated concomitantly with verapamil. At the end of the experiment, ABR was repeated then the animals were sacrificed, and blood samples were obtained for assaying of reduced glutathione and malondialdehyde levels. The left cochlea was processed for scanning electron microscope, while the right cochlea was processed for histopathology and LC3-II immunohistochemistry. Results: Verapamil revealed superiority compared to rapamycin proved by significant improvement in ABR, histopathological results, in addition to its antioxidant effect. Conclusion: verapamil could be suggested as a potential therapeutic approach to decrease gentamicin ototoxicity.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Targeting Autophagy Induction as A possible Protective Mechanism by Verapamil Compared to Rapamycin (Sirolimus) Against Gentamicin -Induced Ototoxicity in Guinea Pigs

Eman Elzayat,

Amany A. Abdin,

Sabiha Hedya

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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“…Natural compounds find application in the treatment of refractory diseases, a new trend in modern clinical medicine (7). Nigella sativa L. is a short-lived annual plant of the Ranunculaceae family, known as black seed, black cumin and fennel flower (8,9). N. sativa is an aromatic plant with tremendous therapeutic properties such as hypotensive, gastroprotective, nephrocurative, nephroprotective, antioxidative, antimicrobial, genoprotective, neuroprotective, immunomodulatory, antiinflammatory, hypoglycemic, hypolipidemic, anticarcinogenic and hepatoprotective (9,10).…”

Section: U N C O R R E C T E D P R O O F Introductionmentioning

confidence: 99%

Assessment of in vitro antigenotoxic effect of nigella sativa oil

Zor¹,

Kurtoğlu²

2020

tjps

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INTRODUCTION: Cyclophosphamide is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of cyclophosphamide has been studied in a variety of in vivo and in vitro systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine Nigella sativa L., (Nigella), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of Nigella sativa oil at different concentrations against the genotoxic effects of Cyclophosphamide by micronucleus test. METHODS: For this purpose, healthy cells were treated in vitro with Nigella sativa oil at concentrations of 1, 5, 10 µg / mL and Cyclophosphamide as positive control for 68 hours. The micronuclei were then counted. RESULTS: No significant increase in micronucleus frequency was observed when the application of Nigella Sativa oil at concentrations of 1, 5, 10 µg / mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 µg / mL) compared to the Cyclophosphamide group in the groups treated with Nigella sativa oil and cyclophosphamide. DISCUSSION AND CONCLUSION: It has been shown that nigella sativa oil may have protective effects against genotoxicity agents in vitro. But more work is needed to understand the mechanism of the genotoxicity effects of nigella sativa oil.

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“…7 Nigella sativa (N. sativa) is a short-lived annual plant of the Ranunculaceae family, known as black seed, black cumin and fennel flower. 8,9 N. sativa is an aromatic plant with tremendous therapeutic properties such as hypotensive, gastroprotective, nephrocurative, nephroprotective, antioxidative, antimicrobial, genoprotective, neuroprotective, immunomodulatory, antiinflammatory, hypoglycemic, hypolipidemic, anticarcinogenic and hepatoprotective. 9,10 It increases the production of two substances, interferon and interleukin, the first defense shield of the immune system against tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Assessment of <i>In Vitro</i> Antigenotoxic Effect of <i>Nigella Sativa</i> Oil

Zor¹,

Kurtoğlu²

2020

tjps

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ÖZAmaç: Siklofosfamid, antineoplastik ve immünosüpresif ajan olarak yaygın olarak kullanılan alkilleyici bir ajandır. Siklofosfamid'in genotoksisitesi çeşitli in vivo ve in vitro sistemde çalışılmış ve rutin olarak genotoksisite testlerinde pozitif kontrol olarak kullanılmaktadır. Geleneksel tıp da Nigella sativa L. (N. sativa), Ranunculaceae familyasından olup günümüzde başta Doğu Akdeniz ülkeleri olmak üzere birçok ülkede yaygın olarak yetişen ve hem baharat hem de halk ilacı olarak Dioscorides zamanından beri kullanılan bir bitkidir. Bu çalışmada Siklofosfamid'in genotoksik etkilerine karşı çörek otu yağının değişik konsantrasyonlardaki koruyucu etkilerininin mikronükleus testi ile gösterilmesi amaçlanmıştır. Gereç ve Yöntemler: Bu amaçla, sağlıklı hücreler 1, 5, 10 µg/mL konsantrasyonlarında N. sativa yağı ile ve pozitif kontrol olarak Siklofosfamid ile 68 saat boyunca in vitro muamele edildi. Daha sonra ise mikronükleuslar sayıldı. Bulgular: N. sativa yağının 1, 5, 10 µg/mL konsantrasyonlarındaki uygulaması negatif kontrolle karşılaştırıldığında mikronükleus frekansında anlamlı bir artış gözlenmedi. N. sativa yağı ve Siklofosfamid'in birlikte uygulandığı gruplarda her üç konsantrasyonda da (1, 5, 10 µg/mL) Siklofosfamid grubuna göre mikronükleus sayısında azalma olduğu görüldü. Sonuç: N. sativa yağının genotoksisite ajanlarına karşı in vitro koruyucu etkilerinin olabileceği gösterilmiştir. N. sativa yağının genotoksisiteyi giderici etkilerinin mekanizmasının anlaşılması için daha fazla çalışmaya ihtiyaç vardır. ABSTRACTObjectives: Cyclophosphamide (CP) is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of CP has been studied in a variety of in vivo and in vitro systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine Nigella sativa L., (N. sativa), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of N. sativa oil at different concentrations against the genotoxic effects of CP by micronucleus test. Materials and Methods: For this purpose, healthy cells were treated in vitro with N. sativa oil at concentrations of 1, 5, 10 µg/mL and CP as positive control for 68 hours. The micronuclei were then counted. Results: No significant increase in micronucleus frequency was observed when the application of N. sativa oil at concentrations of 1, 5, 10 µg/mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 µg/mL) compared to the CP group in the groups treated with N. sativa oil and CP. Conclusion: It has been shown that N. sativa oil may have protective effects against genotoxicity agents in vitro. But more work is needed to understand the mechanism of the genotoxicity effects of N. sativa oil.

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Protective role of intratympanic nigella sativa oil against gentamicin induced hearing loss

Cited by 10 publications

References 37 publications

Targeting Autophagy Induction as A possible Protective Mechanism by Verapamil Compared to Rapamycin (Sirolimus) Against Gentamicin -Induced Ototoxicity in Guinea Pigs

Targeting Autophagy Induction as A possible Protective Mechanism by Verapamil Compared to Rapamycin (Sirolimus) Against Gentamicin -Induced Ototoxicity in Guinea Pigs

Assessment of in vitro antigenotoxic effect of nigella sativa oil

Assessment of <i>In Vitro</i> Antigenotoxic Effect of <i>Nigella Sativa</i> Oil

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