Protective role of glibenclamide against nicotinamidestreptozotocin induced nuclear damage in diabetic Wistar rats

Abstract

Objective:To evaluate the protective effect of glibenclamide against the experimental diabetes-induced nuclear damage in Wistar rats.Materials and Methods:The anti-mutagenic effect of glibenclamide (0.5, 5 and 50 mg/kg, p.o daily for 4 weeks) was evaluated against the nicotinamide (NA)-streptozotocin (STZ) induced type-2 diabetes mellitus using bone marrow micronucleus and sperm abnormalities tests. The antioxidant status was tested by estimating the serum levels of lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx).Results:The results indicated that glibenclamide at 50 mg/kg decreased the frequency of micronuclei in erythrocytes (P < 0.05) and sperm shape abnormality (P < 0.01) besides enhancing the antioxidant status (P < 0.05) in the diabetic rats. However, glibenclamide treatment did not enhance the polychromatic and normochromatic erythrocytes (P/N) ratio and sperm count in the diabetic condition.Conclusion:The observations indicate that the glibenclamide has anti-mutagenic potential which could be related to the antioxidant effect and might also possess anti-proliferative property.

Far-infrared protects vascular endothelial cells from advanced glycation end products-induced injury via PLZF-mediated autophagy in diabetic mice

“…Dose of STZ in the second round of injection was reduced to 50 mg/kg. Administration of nicotinamide before the streptozotocin injection to the mice protects β-cells from STZ-induced severe cytotoxic damages, leading to chronic DM by producing moderate hyperglycemia4243. In the present study, the plasma insulin levels in diabetic mice were much higher than those in normal mice, but did not disappear completely (Supplementary Table S1).…”

Antidiabetic therapies and male reproductive function: where do we stand?

“…Upon induction of a diabetic state through administration of nicotinamidestreptozotocin, the overall fertility parameters were affected showing an increase in shape abnormality and a reduction in sperm count as well as testis weight. However, after treatment with glibenclamide, there was a decrease in shape abnormality, although no changes in sperm count were observed (Rabbani et al 2010b). Similarly, the SU glimepiride was observed to have the same protective effect (Rabbani et al 2009).…”

“…This study has shown their spermicidal potential in vitro by describing a total loss of human sperm viability and motility, possibly due to an elevation of intrasperm calcium concentration ([Ca 2+ ]is), that the authors proved to be resulting from the inhibition of the K ATP channels (Kumar et al 2008). In a different study, it was proved that glibenclamide exerted protective effects on diabetic rats' fertility (Rabbani et al 2010b). Upon induction of a diabetic state through administration of nicotinamidestreptozotocin, the overall fertility parameters were affected showing an increase in shape abnormality and a reduction in sperm count as well as testis weight.…”

“…Both studies suggested a protective role of glibenclamide and glimepiride at sperm level in diabetic conditions. Furthermore, as the authors observed a decrease in oxidative stressrelated parameters after glibenclamide and glimepiride treatment, they suggest that the antioxidant properties of these drugs might reduce the oxidative stressassociated complications, including fertility problems (Rabbani et al 2009(Rabbani et al , 2010b. However, different results were reported by Adaramoye and coworkers that, after glibenclamide treatment in healthy rats, observed a reduction in sperm motility and count while in the testis, lipid peroxidation was increased and the activities of antioxidant enzymes were decreased (Adaramoye et al 2012).…”

“…However, when analyzing the reported results, we can ascertain that when used in vitro, SUs have detrimental effects on capacitation and viability, both in human and mouse sperm cells (Acevedo et al 2006, Kumar et al 2008. On the other hand, when studies were performed in diabetic rats, the administration of SUs showed a positive effect at sperm and testicular level, reversing the adverse effects promoted by the disease (Rabbani et al 2009, 2010b, Nelli et al 2013 (Table 1). Nonetheless, a completely different scenario occurs when studies were performed in non-diabetic rats (Adaramoye et al 2012).…”