Protective Role of Crude Extract of Amorphophallus Campanulatus Against Ethanol-Induced Oxidative Renal Damage

Abstract

Objective:The current study investigates the nephroprotective effect of Amorphophallus campanulatus against chronic alcohol-induced oxidative stress and tissue damage. Methods:The rats were simultaneously supplemented with ethanolic extract of A. campanulatus along with ethanol (40% w/v)2 g/kg body weight/day for 30 days to evaluate the nephroprotective effect against alcohol toxicity. Renal antioxidant enzymes, serum urea, creatinine, and proinflammatory cytokines were assayed biochemically. Histomorphological and histochemical alterations were detected by Hematoxylin and Eosin, periodic acid Schiff, and Feulgen and Picrosirius stain, respectively. The degree of apoptotic cell death was examined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay technique.Results: Serum urea, creatinine, pro-inflammatory cytokines, tissue TBARS, and activity of glutathione metabolizing enzymes were significantly (p<0.01) elevated, whereas cytosolic and mitochondrial superoxide dismutase, catalase, and levels of reduced glutathione were significantly (p<0.001) decreased in the EtOH group compared to control. However, ethanolic extract of A. campanulatus (ACE) supplementation to the EtOH rats reversed these effects to normal levels. Furthermore, degenerative changes in renal cells with alcohol treatment were minimized to nearness in architecture by ACE supplementation. Glycogen and deoxyribonucleic acid depletion, excess fibrosis due to collagen deposition, and increased apoptotic cell number were also restricted by ACE supplementation, with the higher dose being more promising. Conclusion:Thus ethanol-induced nephrotoxicity was attenuated by ACE treatment by the antioxidative and antiapoptotic property of the extract. Such effects of the extract may be due to the probable presence of different bioactive components in the tuber. Hence, it can be used as a regular nutrient or therapeutic agent to protect the renal cells.

Renoprotective effect ofCapsicum annumagainst ethanol‐induced oxidative stress and renal apoptosis

“…For inducing renal toxicity the dose of ethanol was determined to be 40% v/v ethanol, at 2 g per kg body weight (Basu, Das, & Datta, 2013). As described in the guidelines of OECD (TG 407, OECD, 2008) given by CPCSEA, groups of rats ( n = 6) were administered the extract (i.p.)…”

“…For inducing renal toxicity the dose of ethanol was determined to be 40% v/v ethanol, at 2 g per kg body weight (Basu, Das, & Datta, 2013).…”

Genus Amorphophallus: A Comprehensive Overview on Phytochemistry, Ethnomedicinal Uses, and Pharmacological Activities

“…A possible mechanism includes reducing oxidative damage to hepatocytes by reactive oxygen species (ROS) scavenging activity. It also restores and preserves SOD and CAT activity in liver tissue, normalizes liver marker enzyme levels, and reduces GSH depletion and TBARS formation [63]. The polyphenolic compounds identified in A. commutatus extracts were found to exhibit hepatoprotective solid effects; the activities of antioxidant enzymes were elevated, lipid peroxidation was suppressed, and hepatic marker levels decreased in Swiss albino mice models when administered at a dose of 400 mg/kg [64].…”

“…Anthocyanins are natural pigments that are water-soluble and are responsible for the blue, red, and purple coloration of numerous fruits and flowers, which help them attract pollinators [46]. Anthocyanins (58)(59)(60)(61)(62)(63)(64) are also antioxidants that protect plants against oxidative damage [47]. Dietary anthocyanins have been used as protection against some malignancies [48], cardiovascular illnesses [49], and other chronic human issues.…”